Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot FungusPhanerochaete sordidaYK-624

Improvement of ligninolytic properties by recombinant expression of glyoxal oxidase gene in hyper lignin-degrading fungus Phanerochaete sordida YK-624

“…The co-transformed clones (G strains) were screened by PCR, as described previously 19) , with the following modifications: primers PcGLOX F2 and PsGPD R1 were designed to amplify the glx gene fused with the bee2 promoter.…”

8 Degradation and Modification of Plant Biomass by Fungi

“…LiP catalyzes oxidation of a variety of reducing substrates, such as phenols, aromatic amines, aromatic ethers, and polycyclic aromatics (Kirk and Cullen 1998;Hammel and Cullen 2008). Active LiP isozymes and LiP-encoding gene models have been described from only a few genera of white-rot fungi, including Phanerochaete (Glenn et al 1983;Tien and Kirk 1984;Martinez et al 2004;Sugiura et al 2009);Phlebia (Niku-Paavola et al 1988;Vares et al 1995;Hildén et al 2006); Trametes (Johansson and Nyman 1996;Floudas et al 2012); and Bjerkandera (Kimura et al 1991;Heinfling et al 1998a;ten Have et al 1998). Comparative genomic analysis of two Phanerochaete species has shown that P. carnosa has only four LiP-encoding genes, while P. chrysosporium has ten.…”

Genetic engineering, a hope for sustainable biofuel production: review

“…The physical and catalytic properties of the purified enzymes synthesized after transformation are similar to the enzymes isolated from wild type of source. The concentration and time of unset of expression varies according to the species and cultured medium (Sugiura et al, 2009). …”