Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Application in the Deconstruction of Corn Stover

Cai, Jin; Chen, Xiuling; Fan, Jinxia; Huang, Xiaomei; Li, Rui; Sun, Xudong; Li, Qingqing; Li, Dongyu

doi:10.1007/s12010-021-03582-0

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Aspergillus and Trichoderma are well known for the efective production of xylanolytic enzymes [96]. Xylanase-producing fungal sources are summarized in Table 2.…”

Section: Fungal Source Filamentous Fungi Particularlymentioning

confidence: 99%

Xylanase-Producing Microbes and Their Real-World Application

Phuyal,

Budhathoki,

Bista

et al. 2023

International Journal of Chemical Engineering

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The present study is focused on the availability of microbial sources capable of producing xylanase, a hemicelluloses-degrading enzyme with multiple modes of action along with specificity, and their real-world applications. For the accumulation of suitable data, article surfing was carried out using multiple search engines viz. Hinari and PubMed; irrelevant and duplicate articles were discarded and articles were summarized in a narrative way herein. This review article was written aiming to bridge the recent research activities with the commercial activities of xylanase going on around the globe. The readers would be able to acknowledge themselves with the basic idea of the hydrolytic enzyme xylanase, their classification representing their different families, presenting the affinity of different families at the structural level, the sources, and the commercial implications that have been going on alone and in combination. The major hemicellulose, Xylan is digested with the help of combination other enzymes such as alpha-amylase, subtilisin, protease, and endo-1,3(4)-β-glucanase along with xylanase. Xylanase has a diverse applications such as pharmaceutical, food and feed, bakery, paper and pulp, textile, and bio-refinery industries. The objective of this review article is to compile microbial sources of this enzyme and its application for betterment of human kind.

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“…Aspergillus and Trichoderma are well known for the efective production of xylanolytic enzymes [96]. Xylanase-producing fungal sources are summarized in Table 2.…”

Section: Fungal Source Filamentous Fungi Particularlymentioning

confidence: 99%

Xylanase-Producing Microbes and Their Real-World Application

Phuyal,

Budhathoki,

Bista

et al. 2023

International Journal of Chemical Engineering

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“…Several novel xylanases have been identified by metagenomics studies of camel rumen, chicken cecum, termite hindgut, and cow manure [13][14][15][16]. Furthermore, heterologous expressions of these xylanases identified in different hosts by genetic engineering technology could efficiently improve the xylanase yields and exhibit their high thermostability, catalytic efficiency, and organic solvent tolerance [17].…”

Section: Introductionmentioning

confidence: 99%

Mining, Identification, and Characterization of Three Xylanases from the Microbiota of T. fuciformis with Its Companion Strains

Lin,

Li,

Wei

et al. 2023

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Microbial xylanase has wide application in bioenergy, animal feed, environmental protection, the pulp and paper industry, and agricultural development. In this study, three xylanases from the microbiota of T. fuciformis with its companion strains were identified by metagenomics sequencing. The three enzymes were subjected to cloning and expression in E. coli or P. pastoris, purification, and characterization for their properties. The results showed that AsXyn1, from Annulohypoxylon stygium, among the three enzymes possessed high thermostability at 40 °C and broad pH tolerance in the range of 2.0–10.0, exhibiting its application potential. Furthermore, it was found that post-translational modification (such as glycosylation) of AsXyn1 enzyme modulated its activity, kinetic parameters, and thermostability. These results and findings provided a hint for enzyme modification and design in future.

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Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Application in the Deconstruction of Corn Stover

Cited by 2 publications

References 32 publications

Xylanase-Producing Microbes and Their Real-World Application

Xylanase-Producing Microbes and Their Real-World Application

Mining, Identification, and Characterization of Three Xylanases from the Microbiota of T. fuciformis with Its Companion Strains

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