Cloning and gene expression analysis of two cDNA of cysteine proteinase genes involved in programmed cell death in the inner integument from developing seeds of Jatropha curcas L

Rocha, Antônio José; Pohl, Simone; Fonteles, Cristiane Sá Roriz

doi:10.1016/j.gep.2017.12.002

Cited by 4 publications

(2 citation statements)

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“…The mature and enzymatically active KDEL-Cys-EPs exhibit unusual broad substrate specificity (Figure 2A). This characteristic is due to the fact that the active site accepts a wide variety of amino acids, including proline and glycosylated hydroxyproline (e.g., extensions) from the glycoproteins of the CW [41]. When ricinosomes disintegrate and release their content into the cytoplasm, the cells that contain them are going to die [13,14,42].…”

Section: The Role Of Plant-specific Kdel-cys-endopeptidases In Seed Dmentioning

confidence: 99%

Programmed Cell Death in Seeds: An Adaptive Mechanism Required for Life

Matilla¹

2020

Seed Dormancy and Germination

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The regeneration of the mother plant through germinative process is the main reason that evolutionarily justifies the existence of a viable seed. Current knowledge indicates that the control of germination is a sophisticated process mainly controlled by hormones and reactive oxygen species (ROS), among other endogenous factors. One of the events that directly participate in the germination is the degradation of storage proteins (SPs). Thus, vacuolar processing enzymes (VPEs) contribute to SPs' degradation and mobilization due to direct proteolysis or through the activation of other peptidases. In parallel, the relationship between VPEs and programmed cell death (PCD) is beyond doubt. As an alternative to VPEs, the formation of vesicles called ricinosomes containing papain-like Cys-proteases (PLCPs) and located in the reserve tissues of some germinating seeds also collaborates to protein degradation. Finally, there are increasing evidences linking nucleases to PCD in different tissues of seed. However, its state of the art is still little developed. Together, this current overview illustrates a part of the complexity of PCD in seeds, a puzzle far from being solved.

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Section: The Role Of Plant-specific Kdel-cys-endopeptidases In Seed Dmentioning

confidence: 99%

Programmed Cell Death in Seeds: An Adaptive Mechanism Required for Life

Matilla¹

2020

Seed Dormancy and Germination

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“…The growth of biotrophic pathogens is restricted by PCD because these depend on living host tissue to feed on. KDEL-CysEPs participate in developmental PCD, particularly for the elimination of tissues and cells serving transient functions during development, such as megagametophyte cells after germination of white spruce ( Picea glauca ) seeds [17], senescing endosperm tissue of germinating and collapsing nucellus cells of maturing castor bean seeds [2, 3, 9], endosperm cells of imbibed tomato seeds [18], tapetum cells in anthers [19, 20] or Lilium longiflorum tepals [21], the inner integument from developing seeds of Jatropha curcas [22]. Together with nucleases and other proteases, KDEL-CysEPs play a fundamental role in PCD during development (for recent reviews see [23, 24]).…”

Section: Introductionmentioning

confidence: 99%

The role of KDEL-tailed cysteine endopeptidases of Arabidopsis (AtCEP2 and AtCEP1) in root development

et al. 2018

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Plants encode a unique group of papain-type cysteine endopeptidases (CysEP) characterized by a C-terminal KDEL endoplasmic reticulum retention signal (KDEL-CysEP) and an unusually broad substrate specificity. The three Arabidopsis KDEL-CysEPs (AtCEP1, AtCEP2, and AtCEP3) are differentially expressed in vegetative and generative tissues undergoing programmed cell death (PCD). While KDEL-CysEPs have been shown to be implicated in the collapse of tissues during PCD, roles of these peptidases in processes other than PCD are unknown. Using mCherry-AtCEP2 and EGFP-AtCEP1 reporter proteins in wild type versus atcep2 or atcep1 mutant plants, we explored the participation of AtCEP in young root development. Loss of AtCEP2, but not AtCEP1 resulted in shorter primary roots due to a decrease in cell length in the lateral root (LR) cap, and impairs extension of primary root epidermis cells such as trichoblasts in the elongation zone. AtCEP2 was localized to root cap corpses adherent to epidermal cells in the rapid elongation zone. AtCEP1 and AtCEP2 are expressed in root epidermis cells that are separated for LR emergence. Loss of AtCEP1 or AtCEP2 caused delayed emergence of LR primordia. KDEL-CysEPs might be involved in developmental tissue remodeling by supporting cell wall elongation and cell separation.

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