Cloning and functional study of fatty acid-binding protein-like gene of the ridgetail white prawn, Exopalaemon carinicauda

Ma, Hangke; Gao, Huan; Xu, Wanyuan; Sun, Jinqiu; Zhu, Jixuan; Dai, Qin; Hu, Guangwei; Yan, Binlun

doi:10.1007/s10499-020-00540-1

Cited by 13 publications

(3 citation statements)

References 40 publications

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“…This observation agrees with Venkatachalam et al (2017) and Bayir et al (2022), who suggested the iLBPs contained highly conserved lipocalin domains in teleost fish. The lipocalin domain analysis provides supporting evidence that assigned Fabp3, Fabp7a, Fabp7b, Fabp10a, Fabp10b, Fabp11a and Fabp11b are members of the lipocalin superfamily (Ma et al, 2020).…”

Section: Discussionmentioning

confidence: 78%

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Taşbozan

Erbaş

Bayır

et al. 2022

Aquaculture Research

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Vegetable oils (VOs) are preferred by the aquafeed industry as alternative oil sources to fish oil (FO) for sustainable aquaculture production because they decrease catching pressure for wild fish stocks, and compared with FO, VOs have higher annual production, easier accessibility and lower prices (Bayır et al., 2011). VOs are rich sources of 18C fatty acids (FAs), but their n-3 highly unsaturated

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Section: Discussionmentioning

confidence: 78%

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Taşbozan

Erbaş

Bayır

et al. 2022

Aquaculture Research

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“…siRNA synthesis was carried out according to the TR102‐T7 RNAi Transcription Kit (Vazyme). The experimental group was injected with synthetic siRNA at the heart epidermis (5 μg/g) (Ma, Gao, et al, 2020), and the control group was injected with saline (5 μg/g). The ovaries of shrimps were collected at 0, 6, 12, 24, 48 and 72 h after injection ( N = 5).…”

Section: Methodsmentioning

confidence: 99%

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Zhang¹,

Li²,

Zheng³

et al. 2022

Aquaculture Research

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To investigate the key genes affecting ovarian development in the ridgetail white prawn, Exopalaemon carinicauda, we cloned the full‐length cDNA of the CDK9 gene and discussed its role in ovarian development. Our results show that the gene has an open reading frame of 1155 bp, encoded 383 amino acids, and it has a conserved cyclin binding motif S_TKc. The open reading frame of CDK9 was expressed as a 43.96 Kda molecular weight protein. The phylogenetic tree analysis showed that the CDK9 gene has the highest homology with the CDK9 genes of Penaeus vannamei and Penaeus japonicus. The results of qRT‐PCR show that the CDK9 gene was also expressed in the eye stalk, stomach, heart, gill, intestine, muscle, ovary, hepatopancreas and ventral cord. The expression level was highest in the ovary, which was significantly different from the other tissues (p < 0.05). In the five stages of ovarian development, the expression level of the CDK9 gene was highest in stage I, which was significantly different from the other stages (p < 0.05). In situ hybridization tests showed strong expression signals in the membrane, cytoplasm and nucleus of oocytes and previtellogenic oocytes. Nevertheless, following the proliferation of oocytes, the expression signals became much stronger in the oocyte membrane and in the whole follicular cells located in the interstitial space. The RNA interference results show that the expression of the CDK9 gene significantly affects the expression of the Vg and VgR genes. Our findings suggest that CDK9 plays an important role in the synthesis and transportation of vitellin.

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“…The ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) [currently also again referred to as Palaemon carinicauda Holthuis, 1950], is one of the most important economic crustacean species in China because of its fast growth, strong adaptability, delicious taste and rich nutritional value (Wang et al, 2021;Lai et al, 2022b;Zhang et al, 2022). Many studies have been carried out on the shrimp model with regard to its breeding and aquaculture (Wang et al, 2021;Lai et al, 2022a;Pang et al, 2022), and its physiology, biochemistry, and gene functioning (Gao et al, 2017(Gao et al, , 2021Ma et al, 2020;Shen et al, 2021;Lai et al, 2022b).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

Shen

Shen³

et al. 2023

Crustac.

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Actinomycetes, as the main producers of a variety of active metabolites and enzymes, have received extensive attention in aquaculture. An actinomycete had been isolated from Haizhou Bay and been identified by morphological analysis and 16S rRNA sequencing. Results showed that the actinomycete had the highest similarity (99.65%) with Nocardiopsis sp. and was named strain E10. Strain E10 produced an exo-enzyme with proteinase effect that showed strong antibacterial activity against Vibrio anguillarum. This strain E10 was tested as a probiotic for the culture of Exopalaemon carinicauda. After having been fed with a diet supplemented with strain E10 for 30 days, the final body weight (Wt), weight gain rate (WGR), special growth rate (SGR), survival rate (SR) and digestive enzymes (chymotrypsin, α-amylase and lipase) of E. carinicauda in all experimental groups showed no significant differences (). However, the antioxidant enzymes (total superoxide dismutase, catalase and peroxidase) and the non-specific immune-related enzymes (acid phosphatase, alkaline phosphatase and lysozyme) in the treatment groups increased significantly () compared with the control group. The cumulative mortality of the treatment groups (35.56% in 5 × 109 CFU/100 g) was significantly lower than that of the control treatment (73.33%) () post challenge with V. anguillarum for 14 days. The results indicated that strain E10 had no significant effect on the growth, but a significant improvement on the immunity of E. carinicauda. This study provides the data for ecological aquaculture of E. carinicauda.

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Cloning and functional study of fatty acid-binding protein-like gene of the ridgetail white prawn, Exopalaemon carinicauda

Cited by 13 publications

References 40 publications

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

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