Cloning and functional identification of setaria italica somatic embryogenesis receptor-like kinase1 gene (SiSERK1)

The postharvest shelf life of blueberries is very short at room temperature owing to softening, which reduces their edible value. Putrescine (Put) plays an important role in maintaining the firmness and prolonging the storage time of fruits. Therefore, we investigated the relationship between Put and the cell wall metabolism and their roles in the postharvest softening of blueberry. Harvested blueberry fruit was immersed in 1 mM Put aqueous solution for 10 min. After treatment, the blueberries were stored at 20 ± 0.5 °C and 80% relative humidity for 10 days. The results show that Put delayed the softening of the blueberries. Compared to the control, the blueberry fruit treated with Put showed higher levels of firmness and protopectin. Moreover, the activity and expression levels of the cell wall metabolism enzymes were markedly inhibited by the Put treatment, including polygalacturonase (PG), β−galactosylase (β−Gal), and β−glucosidase (β−Glu). The Put treatment promoted the expression of the Put synthesis gene VcODC and inhibited the expression of the Put metabolism gene VcSPDS. Further tests showed that the fruit firmness decreased significantly after the overexpression of VcPG1, which verified that VcPG1 is a key gene for fruit softening. The key transcription factors of fruit softening were preliminarily predicted and the expressions were analyzed, laying a foundation for the subsequent study of transcriptional regulation. These results indicate that Put delays the softening of postharvest blueberry by restraining the cell wall metabolism and maintaining the fruit firmness.

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“…The primer sequences are shown in Table 2 for PCR and sequencing. Gene cloning referred to Wu’s method [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Putrescine Treatment Delayed the Softening of Postharvest Blueberry Fruit by Inhibiting the Expression of Cell Wall Metabolism Key Gene VcPG1

Song

Dai

Wang

et al. 2022

Plants

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“…In our early work, the CDS region of SiSERK1 gene has been constructed into the plant overexpression vector pBIB by Gateway technology and transferred into Agrobacterium tumefaciens LBA4404 [29]. Therefore, only the strain needs to be activated and identi ed in this experiment.…”

Section: Establishment Of Genetic Transformation Systemmentioning

confidence: 99%

“…They have been veri ed from Arabidopsis thaliana [24], rice [25], wheat [26] and other plants [27,28]. In our early study, Seteria italica Somatic embryogenesis receptor-like kinase 1 (SiSERK1) engineering strain has been constructed and overexpressed in a brassinosteroid (BR) receptor mutant of Arabidopsis thaliana WS2 (bri1-5) [29]. It was found that SiSERK1 was involved in BR signaling pathway and plant immune regulation.…”

Section: Introductionmentioning

confidence: 99%

Establishment of in vitro shoot tips regeneration system of foxtail millet and obtainment of transgenic plants of SiSERK1

Tian

et al. 2022

Preprint

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Foxtail millet (Setaria italica) would be suitable as a model plant of C4 plants given its small genome (about 470 MB) and diploid self-pollination. However, the study of foxtail millet faces the problem of low efficiency of explant regeneration and genetic transformation. In this study, a new genetic transformation system of Yugu1 foxtail millet is established with in vitro shoot tips as the explant, and, the concentrations of 6-BA and kanamycin are optimized. It is found 0.5 mg L-1 6-BA and 25 mg L-1 kanamycin are the most suitable in terms of the differentiation rate of shoot tips and survival rate of differentiated seedlings. In addition, 12 transgenic foxtail millets of SiSERK1 are identified by resistance screening and PCR. The insertion site of one line of the transgenic plants chosen at random is further identified. The results of qRT-PCR show that the expression of SiSERK1 gene in transgenic plants is significantly higher than that in wild-type plants. A new method of generation of material for further study of SiSERKs is provided for foxtail millet genetics and breeding.

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mTOR pathway candidate genes and obesity interaction on breast cancer risk in black women from the Women’s Circle of Health Study

Ilozumba

Yaghjyan

Datta

et al. 2023

Cancer Causes Control

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Cloning and functional identification of setaria italica somatic embryogenesis receptor-like kinase1 gene (SiSERK1)

Cited by 3 publications

References 34 publications

Putrescine Treatment Delayed the Softening of Postharvest Blueberry Fruit by Inhibiting the Expression of Cell Wall Metabolism Key Gene VcPG1

Putrescine Treatment Delayed the Softening of Postharvest Blueberry Fruit by Inhibiting the Expression of Cell Wall Metabolism Key Gene VcPG1

Establishment of in vitro shoot tips regeneration system of foxtail millet and obtainment of transgenic plants of SiSERK1

mTOR pathway candidate genes and obesity interaction on breast cancer risk in black women from the Women’s Circle of Health Study

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