Cloning and functional characterization of the promoter ofPsSEOF1gene fromPisum sativumunder different stress conditions usingAgrobacterium-mediated transient assay

Srivastava, Vineet K.; Raikwar, Shailendra; Tuteja, Narendra

doi:10.4161/psb.29626

Cited by 21 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The promoter sequences of five genes, CsC4H, Cs4CL, CsCHS, CsLAR , and CsANR2 , were isolated from tea plants using a reported genome-walker protocol (Vineet Kumar et al, 2014). The length of 1–2 kb nucleotides in the upstream of the coding region of these genes were cloned from leaves.…”

Section: Resultsmentioning

confidence: 99%

“…Primer pairs (Supplementary Table S1) were designed to amplify 1 kb nucleotides from the immediate upstream of these gene coding regions. The Genome-walker kit (Takara, Dalian, China) (Vineet Kumar et al, 2014) was used to isolate these promoter fragments according to the manufacturer’s protocol. The resulting DNA fragments were cloned into the PMD19-T plasmid (Takara, Dalian, China) by following the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach

Guo

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

Green tea (Camellia sinensis, Cs) abundantly produces a diverse array of phenylpropanoid compounds benefiting human health. To date, the regulation of the phenylpropanoid biosynthesis in tea remains to be investigated. Here, we report a cDNA isolated from leaf tissues, which encodes a R2R3-MYB transcription factor. Amino acid sequence alignment and phylogenetic analysis indicate that it is a member of the MYB4-subgroup and named as CsMYB4a. Transcriptional and metabolic analyses show that the expression profile of CsMYB4a is negatively correlated to the accumulation of six flavan-3-ols and other phenolic acids. GFP fusion analysis shows CsMYB4a’s localization in the nucleus. Promoters of five tea phenylpropanoid pathway genes are isolated and characterized to contain four types of AC-elements, which are targets of MYB4 members. Interaction of CsMYB4a and five promoters shows that CsMYB4a decreases all five promoters’ activity. To further characterize its function, CsMYB4a is overexpressed in tobacco plants. The resulting transgenic plants show dwarf, shrinking and yellowish leaf, and early senescence phenotypes. A further genome-wide transcriptomic analysis reveals that the expression levels of 20 tobacco genes involved in the shikimate and the phenylpropanoid pathways are significantly downregulated in transgenic tobacco plants. UPLC-MS and HPLC based metabolic profiling reveals significant reduction of total lignin content, rutin, chlorogenic acid, and phenylalanine in CsMYB4a transgenic tobacco plants. Promoter sequence analysis of the 20 tobacco genes characterizes four types of AC-elements. Further CsMYB4a-AC element and CsMYB4a-promoter interaction analyses indicate that the negative regulation of CsMYB4a on the shikimate and phenylpropanoid pathways in tobacco is via reducing promoter activity. Taken together, all data indicate that CsMYB4a negatively regulates the phenylpropanoid and shikimate pathways.Highlight: A tea (Camellia sinensis) MYB4a is characterized to encode a R2R3-MYB transcription factor. It is shown to repressively control the phenylpropanoid and shikimate pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach

Guo

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Furthermore, some proteins such as sieve element occlusion 1 (SEO1), SEO2, and SEO3 are located in the phloem and reported to prevent efficiently aphid feeding in M. truncatula ( Will et al , 2013 ). The SEO genes can be up-regulated by dehydration and artificial ABA treatment in the legume Pisum sativum ( Srivastava et al , 2014 ). Our results indicate that up-regulation of the JA signaling pathway and other defensive metabolites may increase the phloem defense during aphid penetration under drought conditions.…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of abscisic acid signaling pathway facilitates aphid xylem absorption and osmoregulation under drought stress

Guo

Sun

Peng

et al. 2015

EXBOTJ

View full text Add to dashboard Cite

show abstract

“…In the past few years, great progress has been done on the identification and evaluation of plant-derived tissue-specific and stress-inducible promoters. [19][20][21] But very few promoters conferring trichome-specific or preferential expression which include promoters of Arabidopsis OASA1 and AtTSG1, 22,23 lipid transfer protein genes of cotton and tobacco, [24][25][26] and tobacco CYP71D16, 27 have been isolated and characterized in plants. Promoter of A. annua ADS gene was found to drive expression in the anthers and trichomes of Arabidopsis juvenile leaves, 28 while exclusively in stalk and secretory cells of A. annua glandular trichomes.…”

Section: Short Communicationmentioning

confidence: 99%

Promoters ofAaGL2andAaMIXTA-Like1genes ofArtemisia annuadirect reporter gene expression in glandular and non-glandular trichomes

Jindal¹,

Longchar²,

Singh

et al. 2015

Plant Signaling & Behavior

View full text Add to dashboard Cite

Herein, we report cloning and analysis of promoters of GLABRA2 (AaGL2) homolog and a MIXTA-Like (AaMIXTA-Like1) gene from Artemisia annua. The upstream regulatory regions of AaGL2 and AaMIXTA-Like1 showed the presence of several crucial cis-acting elements. Arabidopsis and A. annua seedlings were transiently transfected with the promoter-GUS constructs using a robust agro-infiltration method. Both AaGL2 and AaMIXTA-Like1 promoters showed GUS expression preferentially in Arabidopsis single-celled trichomes and glandular as well as T-shaped trichomes of A. annua. Transgenic Arabidopsis harboring constructs in which AaGL2 or AaMIXTA-Like1 promoters would control GFP expression, showed fluorescence emanating specifically from trichome cells. Our study provides a fast and efficient method to study trichome-specific expression, and 2 promoters that have potential for targeted metabolic engineering in plants.

show abstract

Cloning and functional characterization of the promoter ofPsSEOF1gene fromPisum sativumunder different stress conditions usingAgrobacterium-mediated transient assay

Cited by 21 publications

References 33 publications

Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach

Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach

Up-regulation of abscisic acid signaling pathway facilitates aphid xylem absorption and osmoregulation under drought stress

Promoters ofAaGL2andAaMIXTA-Like1genes ofArtemisia annuadirect reporter gene expression in glandular and non-glandular trichomes

Contact Info

Product

Resources

About