Cloning and functional characterization of two GsSnRK1 gene promoters from wild soybean

Chen, Jun; Li, Qiang; Zhang, Pengmin; Lu, Haoran; Bian, Yaoyao; Yi, Jine; Wang, Yizhu; Ding, Xiaodong; Xiao, Jialei

doi:10.1007/s11816-021-00700-6

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…In plants, SnRK family kinases are classified into SnRK1, SnRK2, and SnRK3 subfamilies ( Wang et al., 2019b ). In G. soja , there are four SnRK1 genes named GsSnRK1.1-1.4 ( Chen et al., 2021b ). Among them, the expression of GsSnRK1.1 and GsSnRK1.2 were induced by both salt (NaCl) and bicarbonate alkaline (NaHCO 3 ) stresses, while GsSnRK1.3 and GsSnRK1.4 were not affected by either salt or bicarbonate alkaline stress.…”

Section: Protein Kinases Involved In Salt-alkaline Stress Response In...mentioning

confidence: 99%

Insights into the regulation of wild soybean tolerance to salt-alkaline stress

Cai

Jia²,

Sun³

et al. 2022

Front. Plant Sci.

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Soybean is an important grain and oil crop. In China, there is a great contradiction between soybean supply and demand. China has around 100 million ha of salt-alkaline soil, and at least 10 million could be potentially developed for cultivated land. Therefore, it is an effective way to improve soybean production by breeding salt-alkaline-tolerant soybean cultivars. Compared with wild soybean, cultivated soybean has lost a large number of important genes related to environmental adaptation during the long-term domestication and improvement process. Therefore, it is greatly important to identify the salt-alkaline tolerant genes in wild soybean, and investigate the molecular basis of wild soybean tolerance to salt-alkaline stress. In this review, we summarized the current research regarding the salt-alkaline stress response in wild soybean. The genes involved in the ion balance and ROS scavenging in wild soybean were summarized. Meanwhile, we also introduce key protein kinases and transcription factors that were reported to mediate the salt-alkaline stress response in wild soybean. The findings summarized here will facilitate the molecular breeding of salt-alkaline tolerant soybean cultivars.

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Section: Protein Kinases Involved In Salt-alkaline Stress Response In...mentioning

confidence: 99%

Insights into the regulation of wild soybean tolerance to salt-alkaline stress

Cai

Jia²,

Sun³

et al. 2022

Front. Plant Sci.

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“…To determine whether the ZmATG3 promoter responds to abiotic stress, we cloned the 2000 bp promoter of ZmATG3 , ligated it into pUBI10-GUS (Beyotime Biotechnology, Shanghai, China), and transferred it into Agrobacterium strain GV3101 to generate ZmATG3-GUS transgenic Arabidopsis. Twelve-day-old ZmATG3-GUS transgenic Arabidopsis seedlings were transferred to 1/2 MS medium containing NaCl (100 mM) [ 31 ] and mannitol (200 mM) [ 32 ]. The first leaf of each plant was collected after 0, 12, and 24 h of treatment.…”

Section: Methodsmentioning

confidence: 99%

Maize Autophagy-Related Protein ZmATG3 Confers Tolerance to Multiple Abiotic Stresses

Liu,

Ma,

Tang

et al. 2024

Plants

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Abiotic stresses pose a major increasing problem for the cultivation of maize. Autophagy plays a vital role in recycling and re-utilizing nutrients and adapting to stress. However, the role of autophagy in the response to abiotic stress in maize has not yet been investigated. Here, ZmATG3, which is essential for ATG8-PE conjugation, was isolated from the maize inbred line B73. The ATG3 sequence was conserved, including the C-terminal domains with HPC and FLKF motifs and the catalytic domain in different species. The promoter of the ZmATG3 gene contained a number of elements involved in responses to environmental stresses or hormones. Heterologous expression of ZmATG3 in yeast promoted the growth of strain under salt, mannitol, and low-nitrogen stress. The expression of ZmATG3 could be altered by various types of abiotic stress (200 mM NaCl, 200 mM mannitol, low N) and exogenous hormones (500 µM ABA). GUS staining analysis of ZmATG3-GUS transgenic Arabidopsis revealed that GUS gene activity increased after abiotic treatment. ZmATG3-overexpressing Arabidopsis plants had higher osmotic and salinity stress tolerance than wild-type plants. Overexpression of ZmATG3 up-regulated the expression of other AtATGs (AtATG3, AtATG5, and AtATG8b) under NaCl, mannitol and LN stress. These findings demonstrate that overexpression of ZmATG3 can improve tolerance to multiple abiotic stresses.

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Functional characterization of wild soybean (Glycine soja) GsSnRK1.1 protein kinase in plant resistance to abiotic stresses

Liu

Cao

et al. 2023

Journal of Plant Physiology

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Cloning and functional characterization of two GsSnRK1 gene promoters from wild soybean

Cited by 3 publications

References 34 publications

Insights into the regulation of wild soybean tolerance to salt-alkaline stress

Insights into the regulation of wild soybean tolerance to salt-alkaline stress

Maize Autophagy-Related Protein ZmATG3 Confers Tolerance to Multiple Abiotic Stresses

Functional characterization of wild soybean (Glycine soja) GsSnRK1.1 protein kinase in plant resistance to abiotic stresses

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