Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina

He, Qinghua; Qiao, Dan; Zhang, Qinglian; Yin, Li; Xu, Hui; Liang, Wei; Gu, Ying; Cao, Yi

doi:10.1023/b:japh.0000047950.76549.ce

Cited by 22 publications

(17 citation statements)

References 20 publications

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“…The stress induction of glycolysis transcripts has been reported in other studies (36). Regarding the unstable Nipponbare 100-Gy line (Table 1), we also found, in the different functional groups, some genes already associated with stress/ defense responses, specifically to NaCl-stress response: several aquaporin and lipid transfer proteins (37), one high-affinity nitrate transporter (38), one glycine-rich cell wall protein (24), and one endo-1,3-1,4-␤-D-glucanase (39). The altered expression of the photosynthesis-associated genes encoding photosystem II protein D2 and chloroplast ATP synthase is consistent with the already known effect of ␥-irradiation on the photosynthetic activity (40).…”

Section: (Continued)supporting

confidence: 82%

Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion

Batista

Saibo

Lourenço

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

145

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Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other plants that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such as mutagenesis). What is beyond the phenotype of these improved plants? Should mutagenized plants be treated differently from transgenics? We have evaluated the extent of transcriptome modification occurring during rice improvement through transgenesis versus mutation breeding. We used oligonucleotide microarrays to analyze gene expression in four different pools of four types of rice plants and respective controls: (i) a ␥-irradiated stable mutant, (ii) the M1 generation of a 100-Gy ␥-irradiated plant, (iii) a stable transgenic plant obtained for production of an anticancer antibody, and (iv) the T1 generation of a transgenic plant produced aiming for abiotic stress improvement, and all of the unmodified original genotypes as controls. We found that the improvement of a plant variety through the acquisition of a new desired trait, using either mutagenesis or transgenesis, may cause stress and thus lead to an altered expression of untargeted genes. In all of the cases studied, the observed alteration was more extensive in mutagenized than in transgenic plants. We propose that the safety assessment of improved plant varieties should be carried out on a case-by-case basis and not simply restricted to foods obtained through genetic engineering.food safety evaluation ͉ rice ͉ genetically modified organisms ͉ genetic engineering ͉ ␥-irradiation

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Section: (Continued)supporting

confidence: 82%

Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion

Batista

Saibo

Lourenço

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

145

View full text Add to dashboard Cite

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“…The limiting agent could have been supplied with the wastewater effluent, allowing the phytoplankton at the downstream location to grow. Alternatively, if the limiting agent did not affect NH 4 + assimilation, the phytoplankton at the downstream location could have been utilizing NH 4 + exclusively, which was on average 300-fold greater than upstream and is consistent with a general preference for NH 4 + over NO 3 − by phytoplankton (Cresswell & Syrett 1979, Syrett 1981, Fernandez & Cardenas 1989, Huppe et al 1994, Berges et al 1995, Hildebrand & Dahlin 2000, He et al 2004, Song & Ward 2007.…”

Section: R H O D O M O N a S S Pmentioning

confidence: 73%

Impact of atypical ammonium concentrations on phytoplankton abundance and composition in fresh versus estuarine waters

Esparza¹,

Farrell²,

Craig³

et al. 2014

Aquat. Biol.

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“…Moreover, it is a simple method to switch on or off the expression of the heterologous genes under the control of the inducible promoters. Based on the physiological traits of the NR genes from the green algae Chlamydomonas (Koblenz and Lechtreck 2005), Chlorella (Wang et al 2004) and Dunaliella (Song and Ward 2004;He et al 2004;Sun et al 2006;Jia et al 2005), which is induced by nitrate but repressed by ammonium, the promoter of the NR gene from D. salina can be used as an inducible promoter to control the expression of the heterologous genes in transgenic D. salina.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the NR of D. salina is located in pyrenoids and regulated by light (Lopez-Ruiz et al 1985;Jimenez et al 1993). He et al (2004) also showed that the expression of a nitrate transporter gene was induced by nitrate and repressed by ammonium in D. salina. Therefore, the upstream region of the NR gene from D. salina may be an ideal promoter for the inducible expression of heterologous genes.…”

Section: Introductionmentioning

confidence: 89%

Inducible EGFP expression under the control of the nitrate reductase gene promoter in transgenic Dunaliella salina

Xue

Yan

et al. 2007

J Appl Phycol

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The upstream region of the nitrate reductase (NR) gene is able to provide an ideal promoter for inducible expression of the heterologous genes because NR transcripts are induced by nitrate and repressed by ammonium. To improve the expression of heterologous proteins in transgenic Dunaliella salina, we developed a novel vector with the ability to switch on or off the expression of the heterologous genes using the upstream region of the NR gene. In the present study, a 1.2-kb fragment containing the promoter (Pnr) and a 0.9-kb fragment containing the terminator (Tnr) of the NR gene were cloned from the genome of D. salina. The results of mobility shift assays indicated that a GATA element in the isolated Pnr was involved in the nitrogen-dependent regulation. Additionally, the transcription start site of the D. salina NR gene was located at 133 bp upstream of the start codon ATG. Gene splicing by overlap extension (SOEing) was employed to construct a recombinant vector, p7NET, containing a Pnr-EGFP-Tnr expression cassette. On day 2 after the p7NET was introduced into the D. salina cells, EGFP-expressing transformants of D. salina appeared, in which expression of EGFP was induced by nitrate but repressed by ammonium. RT-PCR and PCR-Southern blots showed that the EGFP gene was correctly transcribed in transgenic D. salina. The findings of the present study suggest that the promoter of the NR gene from D. salina may be used to drive the inducible expression of heterologous proteins in transgenic D. salina.

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Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina

Cited by 22 publications

References 20 publications

Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion

Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion

Impact of atypical ammonium concentrations on phytoplankton abundance and composition in fresh versus estuarine waters

Inducible EGFP expression under the control of the nitrate reductase gene promoter in transgenic Dunaliella salina

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