Cloning and expression profiling of small RNAs expressed in the mouse ovary

Ro, Seungil; Song, Rui; Park, Chanjae; Zheng, Huili; Sanders, Kenton M.; Yan, Wei

doi:10.1261/rna.754207

Cited by 174 publications

(151 citation statements)

References 35 publications

(77 reference statements)

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“…6) showed that bta-miR-144, bta-miR-451, bta-miR-652, and bta-miR-873 were present in tissues across the bovine body, with levels of all these miRNAs except bta-miR-873 being relatively low in the ovary. In contrast, bta-miR-202 was not expressed in tissues other than testis and ovary, consistent with results in other species (Landgraf et al 2007, Ro et al 2007, Armisen et al 2009, Juanchich et al 2013.…”

Section: Localization Of Mirnas Within Follicular Compartmentssupporting

confidence: 90%

“…With a few exceptions (Carletti et al 2010, Kitahara et al 2013, most evidence on follicular roles of miRNAs has been obtained using cultured cells, particularly rodent cells, and in some cases actual changes in the expression of these miRNAs during follicle development have not been demonstrated. Indeed, although genome-wide miRNA analyses of whole ovarian tissues (Landgraf et al 2007, Ro et al 2007, Mishima et al 2008, Hossain et al 2009, Ahn et al 2010, Juanchich et al 2013 or follicular, or luteal tissues (Fiedler et al 2008, McBride et al 2012, Miles et al 2012, da Silveira et al 2012, Donadeu & Schauer 2013, Schauer et al 2013, Sohel et al 2013 have been reported in several species, detailed spatiotemporal profiles encompassing several follicle developmental stages have not. Identifying such profiles will be an important step toward understanding the functional involvement of miRNAs during specific stages of follicle development.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

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Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4-8 mm) or large (12-17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (nZ6) and large atretic (nZ5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P!0.05) between large healthy and each of small and large atretic follicles respectively. Bovine miRNAs confirmed to be upregulated in large healthy follicles relative to small follicles (bta-miR-144, bta-miR-202, bta-miR-451, bta-miR-652, and bta-miR-873) were further characterized. Three of these miRNAs (bta-miR-144, bta-miR-202, and bta-miR-873) were also downregulated in large atretic follicles relative to large healthy follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Further, body-wide screening revealed that bta-miR-202, but not other miRNAs, was expressed exclusively in the gonads. Finally, a total of 1359 predicted targets of the five miRNAs enriched in large healthy follicles were identified, which mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, prevention of premature luteinization, and oocyte maturation.

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Section: Localization Of Mirnas Within Follicular Compartmentssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

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“…A number of tissue-specific snoRNAs have been identified that are believed to be involved in the regulation of gene expression [48]. Ro et al [49] identified novel snoRNAs that were preferentially expressed in the ovary, suggesting that they might have a role limited to ovarian functions. Further studies are needed in order to reveal their potential physiological role in folliculogenesis.…”

Section: Discussionmentioning

confidence: 99%

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Papler

Bokal

Tacer

et al. 2013

J Assist Reprod Genet

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Purpose The aim of our study was to determine whether there are any differences in the cumulus cell gene expression profile of mature oocytes derived from modified natural IVF and controlled ovarian hyperstimulation cycles and if these changes could help us understand why modified natural IVF has lower success rates. Methods Cumulus cells surrounding mature oocytes that developed to morulae or blastocysts on day 5 after oocyte retrieval were submitted to microarray analysis. The obtained data were then validated using quantitative real-time PCR. Results There were 66 differentially expressed genes between cumulus cells of modified natural IVF and controlled ovarian hyperstimulation cycles. Gene ontology analysis revealed the oxidation-reduction process, glutathione metabolic process, xenobiotic metabolic process and gene expression were significantly enriched biological processes in MNIVF cycles.Among differentially expressed genes we observed a large group of small nucleolar RNA's whose role in folliculogenesis has not yet been established. Conclusion The increased expression of genes involved in the oxidation-reduction process probably points to hypoxic conditions in modified natural IVF cycles. This finding opens up new perspectives for the establishment of the potential role that oxidation-reduction processes have in determining success rates of modified natural IVF.

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“…Small RNA populations have been identified by cloningbased or next-generation sequencing of normal ovarian tissues from human (Landgraf et al 2007), mice (Ro et al 2007, Mishima et al 2008, Ahn et al 2010, pigs , cattle (Hossain et al 2009, Tripurani et al 2010, Huang et al 2011, Miles et al 2012 and sheep (McBride et al 2012). Most of those studies involved analyses on whole ovaries rather than on specific ovarian tissue components, an approach that, although very useful for comprehensive identification of miRNA sequences, provides very limited insight into their functional relevance.…”

Section: Identification and Profiling Of Mirnas In Ovarian Tissuesmentioning

confidence: 99%

“…Most of those studies involved analyses on whole ovaries rather than on specific ovarian tissue components, an approach that, although very useful for comprehensive identification of miRNA sequences, provides very limited insight into their functional relevance. miRNAs constitute the most abundant class of small RNAs in the ovary (Ro et al 2007, Mishima et al 2008, Ahn et al 2010, Huang et al 2011. In a study using porcine tissues, as many as 673 different miRNA sequences were identified by deep sequencing of whole ovaries , although not all may be bona fide miRNAs (Kozomara & Griffiths-Jones 2011).…”

Section: Identification and Profiling Of Mirnas In Ovarian Tissuesmentioning

confidence: 99%

Involvement of miRNAs in ovarian follicular and luteal development

Donadeu¹,

Schauer²,

Sontakke³

2012

Journal of Endocrinology

162

110

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Although much progress has been made in the genetic dissection of biological networks involved in follicular/luteal development in the mammalian ovary, the gene regulation mechanisms involved are still poorly understood. Over the last 10 years, miRNAs have emerged as master regulators of tissue growth and differentiation in animals. However, compared with other body tissues, little is still known about the functional involvement of miRNAs in the ovary. Several studies have identified miRNA populations specifically associated with the development of follicles and corpora lutea, particularly in relation to the follicular-luteal transition, and the functional involvement of some of these miRNAs has been characterised in vitro and/or in vivo. Specifically, three different miRNAs, miR-224, miR-378 and miR-383, have shown to be involved in regulating aromatase expression during follicle development. In addition, miR-21 has been identified as promoting follicular cell survival during ovulation, and pro-angiogenic miR-17-5p and let-7b were shown to be necessary for normal development of the corpus luteum. Experimental evidence for the involvement of several other miRNAs in different aspects of follicle/luteal development has also been obtained. In addition, many of these studies exemplify the challenges associated with identifying physiologically relevant targets of ovarian miRNAs. Continuous advances in this field will be considerably facilitated by progress in understanding miRNA physiology in other body systems and will eventually lead to a much better understanding of the control of follicular/luteal development. In turn, through the potential offered by miRNA diagnostics and miRNA therapeutics, this new knowledge should bring considerable benefits to reproductive medicine.

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Cloning and expression profiling of small RNAs expressed in the mouse ovary

Cited by 174 publications

References 35 publications

Characterization of microRNAs differentially expressed during bovine follicle development

Characterization of microRNAs differentially expressed during bovine follicle development

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles

Involvement of miRNAs in ovarian follicular and luteal development

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