Cloning and Expression of Two Carbonyl Reductase-like 20β-Hydroxysteroid Dehydrogenase cDNAs in Ovarian Follicles of Rainbow Trout (Oncorhynchus mykiss)

Guan, Guijun; Tanaka, Minoru; Todo, Takashi; Young, Graham; Yoshikuni, Michiyasu; Nagahama, Yoshitaka

doi:10.1006/bbrc.1998.0127

Cited by 54 publications

(32 citation statements)

References 15 publications

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“…On the other hand, the features typical for carbonyl reductase like 20b-HSD were conserved in Atlantic cod, strongly suggesting that the sequence determined in this study indeed represents the 20b-hsd gene of Atlantic cod. In agreement with the data obtained for rainbow trout (Guan et al 1999) and Nile tilapia , 20b-hsd was quite ubiquitously expressed. However, there seemed to be a sex-specific variation in 20b-hsd expression in heart and gills in Atlantic cod, in addition to the expected large differences in the gonads.…”

Section: Discussionsupporting

confidence: 90%

“…The protein encoded by this gene belongs to the shortchain steroid dehydrogenase/reductase superfamily (Jörnvall et al 1995) and catalyzes the NADPH reduction of carbonyl compounds and steroids. The gene is broadly expressed in a variety of fish tissues (Guan et al 1999, Wang & Ge 2002, and it is evident that the 20b-HSD represents the key enzyme for the production of MIS (Tanaka et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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20β-hydroxysteroid dehydrogenase and CYP19A1 are differentially expressed during maturation in Atlantic cod (Gadus morhua)

Mittelholzer¹,

Andersson²,

Consten³

et al. 2007

Journal of Molecular Endocrinology

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In order to better quantify the molecular mechanisms regulating final oocyte maturation and spawning, complete coding sequences with partially or fully untranslated regions for the steroidogenic enzymes, cytochrome P450 aromatase and 20b-hydroxysteroid dehydrogenase, were cloned from ovaries of Atlantic cod (Gadus morhua). The nucleotide and amino acid sequences showed high homologies with the corresponding sequences of other fish species, and conserved features important for functionality were identified in both predicted proteins. The sequences of the corresponding genomic loci were also determined, allowing the design of mRNA-specific quantitative PCR assays. As a reference gene for the real-time RT-PCR assays, eukaryotic elongation factor 1a was chosen, and the mRNA as well as the genomic sequence was determined. In addition, a real-time quantitative PCR assay for the 18S rRNA was adapted to be used in cod. Analysis of immature and maturing female cod from July to January respectively showed that the enzyme genes showed the expected quantitative changes associated with physiological regulation. However, mRNA for eukaryotic elongation factor 1a, and to a lesser extent even 18S rRNA, showed variable expression in these samples as well. To find accurate standards for real-time PCR in such a dynamic organ as the cod ovary is not an easy task, and several possible solutions are discussed.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

20β-hydroxysteroid dehydrogenase and CYP19A1 are differentially expressed during maturation in Atlantic cod (Gadus morhua)

Mittelholzer¹,

Andersson²,

Consten³

et al. 2007

Journal of Molecular Endocrinology

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“…The active progestin in fish is 17 ,20 -dihydroxyprogesterone, which is not active in either in birds or mammals because the addition of a 17 -hydroxy group to progesterone reduces its affinity for these PRs to less than 1% of that of progesterone (Smith et al, 1974). Fish contain a 20 -hydroxysteroid dehydrogenase, which can increase the local concentration of the active progestin [Guan et al, 1999]. In mammals, expression of this enzyme will yield an inactive progestin, thus, quenching progestin action.…”

Section: Fuzzy Recognition Of Steroids By Receptors In Fishmentioning

confidence: 99%

Co-evolution of steroidogenic and steroid-inactivating enzymes and adrenal and sex steroid receptors

Baker

2004

Molecular and Cellular Endocrinology

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Abstract. Receptors for the adrenal and sex steroids arose by a series of gene duplications from an ancestral nuclear receptor in a primitive vertebrate, at least 540 million years ago. Sequence analysis indicates many steroidogenic and steroid-inactivating enzymes, including cytochrome P450s and hydroxysteroid dehydrogenases, arose at the same time. The estrogen receptor (ER) appears to be the ancestral steroid receptor. Initially, the redundant duplicated ER had a low specificity for its new ligand. This raises the question: "How was specificity for responses to different steroids regulated early in the evolution of steroid receptors?" Selective expression of these steroid-metabolizing enzymes provided specificity for different steroid responses in primitive vertebrates. 17 -hydroxysteroid dehydrogenase-type 1 (17 -HSD-type 1) and 17 -HSD-type 2, which preferentially catalyze the reduction and oxidation at C17 of androgens and estrogens, respectively, provide an example of this mechanism. Selective expression of either 17 -HSD-type 1 or 17 -HSD-type 2 can regulate synthesis or inactivation of androgens or estrogens in specific cells.Steroids also were important in the evolution of land animals, which began about 400 million years ago.Steroidogenic and steroid-inactivating enzymes were recruited to regulate steroid-mediated responses as organ function became more complex. For example, in the kidney 11 -HSD-type 2 prevents binding of glucocorticoids to the mineralocorticoid receptor, which is crucial for aldosterone-mediated regulation of electrolyte transport in the distal tubule. We propose that 5 steroids, such as dehydroepiandrosterone and its metabolites, were the ligands for the ancestral ER. Understanding the actions of 5 steroids in amphioxus and lamprey may shed light on adrenarche and neurosteroid actions in humans.

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“…Salmonids are excellent study animals to examine the changes in steroidogenic enzyme gene expression in relation to steroid hormone synthesis, since germ cell development progresses in a synchronous fashion and dynamic changes in steroid hormone secretion occur during gametogenesis. In rainbow trout, cDNAs for most of the steroidogenic enzymes responsible for the synthesis of sex steroids have already been isolated: cytochrome P450 cholesterol side-chain cleavage (P450scc) (Takahashi et al 1993), cytochrome P450 17 -hydroxylase/17,20-lyase (P450C17) (Sakai et al 1992), 3 -hydroxysteroid dehydrogenase/n5-4-isomerase (3 -HSD) (Sakai et al 1994), cytochrome P450 aromatase (P450 arom) , cytochrome P450 11 -hydroxylase (P45011 ) (Liu et al 2000, Kusakabe et al 2002a, 11 -hydroxysteroid dehydrogenase (11 -HSD) (Kusakabe et al 2003) and 20 -hydroxysteroid dehydrogenase (20 -HSD) (Guan et al 1999). Recent studies reported changes in mRNA levels for key ovarian steroidogenic enzymes during a reproductive cycle in channel catfish (Kumar et al 2000), Arctic char (von Hofsten et al 2002) and rainbow trout (Nakamura et al 2005), and a few recent studies in male teleosts reported seasonal changes in transcript levels of a single steroidogenic enzyme (Liu et al 2000, Kusakabe et al 2002a.…”

Section: Introductionmentioning

confidence: 99%

Changes in mRNAs encoding steroidogenic acute regulatory protein, steroidogenic enzymes and receptors for gonadotropins during spermatogenesis in rainbow trout testes

Kusakabe¹,

Nakamura²,

Evans³

et al. 2006

Journal of Endocrinology

109

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In vertebrates, sperm development and maturation are directly regulated by gonadal steroid hormone secretion. The relationships among the expression of genes encoding steroidogenic proteins and receptors for gonadotropins, and testicular steroid production have not yet been comprehensively determined in male teleosts. In this study, the changes in levels of mRNAs encoding follicle-stimulating hormone (FSH) receptor, luteinizing hormone (LH) receptor, steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage, 3 -hydroxysteroid dehydrogenase/n5-4-isomerase, cytochrome P450 17 -hydroxylase/17,20-lyase, cytochrome P450 11 -hydroxylase, 11 -hydroxysteroid dehydrogenase and 20 -hydroxysteroid dehydrogenase were determined by real-time, quantitative PCR assays and related to changes in serum steroid levels throughout the reproductive cycle in male rainbow trout. Serum 11-ketotestosterone and 17 ,20 -dihydroxy-4-pregnen-3-one levels were measured by RIA. Although the pattern of change in the mRNA levels for the enzymes was variable, the increases in steroidogenic enzyme mRNAs started prior to a significant increase of serum steroid levels. The patterns of transcript levels of FSH and LH receptors suggest that changes in StAR and steroidogenic enzyme transcripts are largely mediated by the FSH receptor during early and mid-spermatogenesis and by the LH receptor during late spermatogenesis and spermiation. Levels of StAR (10-fold) and P450 17 -hydroxylase/ 17,20-lyase (sevenfold) transcripts changed with the greatest magnitude and were closely related to the changes in serum steroids, suggesting that changes in StAR and P450 17 -hydroxylase/17,20-lyase abundance are likely to be the major influences on overall steroidogenic output during the reproductive cycle in male rainbow trout.

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Cloning and Expression of Two Carbonyl Reductase-like 20β-Hydroxysteroid Dehydrogenase cDNAs in Ovarian Follicles of Rainbow Trout (Oncorhynchus mykiss)

Cited by 54 publications

References 15 publications

20β-hydroxysteroid dehydrogenase and CYP19A1 are differentially expressed during maturation in Atlantic cod (Gadus morhua)

20β-hydroxysteroid dehydrogenase and CYP19A1 are differentially expressed during maturation in Atlantic cod (Gadus morhua)

Co-evolution of steroidogenic and steroid-inactivating enzymes and adrenal and sex steroid receptors

Changes in mRNAs encoding steroidogenic acute regulatory protein, steroidogenic enzymes and receptors for gonadotropins during spermatogenesis in rainbow trout testes

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