Cloning and Expression of Two Carboxylesterases, and Their Activity Modulation in Chinese Mitten crab Eriocheir sinensis under Pesticide Exposer

Zang, Yanan; Xiao, Zhigang; Huang, Pengdan; Hashim, Kassimu; Shen, Huangxuan

doi:10.4194/1303-2712-v20_7_02

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…We assumed that M. rosenbergii may mitigate the toxicity of intracellular Cu accumulation by sequestering, utilizing, and excreting excess Cu. In addition, JHE-like carboxylesterase 2 (CXE2) and cytochrome P 450 (CYP 450) are involved in detoxification and protection against oxidative damage in crustaceans [63,64]. Cu exposure also induced the expression of CYP450 (CL1886.Contig2_All) and CXE2 (CL426.Contig5_All).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Zhang,

Bao,

Guo

et al. 2024

Animals

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The contamination of aquatic ecosystems by the heavy metal copper (Cu) is an important environmental issue and poses significant risks to the physiological functions of aquatic organisms. Macrobrachium rosenbergii is one of the most important freshwater-cultured prawns in the world. The hepatopancreas of crustaceans is a key organ for immune defense, heavy metal accumulation, and detoxification, playing a pivotal role in toxicological research. However, research on the molecular response of the hepatopancreas in M. rosenbergii to Cu exposure is still lacking. In this study, the transcriptomic response in the hepatopancreas of M. rosenbergii was studied after Cu exposure for 3 and 48 h. Compared with the control group, 11,164 (7288 up-regulated and 3876 down-regulated genes) and 10,937 (6630 up-regulated and 4307 down-regulated genes) differentially expressed genes (DEGs) were identified after 3 and 48 h exposure, respectively. Most of these DEGs were up-regulated, implying that gene expressions were largely induced by Cu. Functional enrichment analysis of these DEGs revealed that immunity, copper homeostasis, detoxification, DNA damage repair, and apoptosis were differentially regulated by Cu. Seven genes involved in immunity, detoxification, and metabolism were selected for validation by qRT-PCR, and the results confirmed the reliability of RNA-Seq. All these findings suggest that M. rosenbergii attempts to resist the toxicity of Cu by up-regulating the expression of genes related to immunity, metabolism, and detoxification. However, with the excessive accumulation of reactive oxygen species (ROS), the antioxidant enzyme system was destroyed. As a result, DNA damage repair and the cellular stress response were inhibited, thereby exacerbating cell damage. In order to maintain the normal function of the hepatopancreas, M. rosenbergii removes damaged cells by activating the apoptosis mechanism. Our study not only facilitates an understanding of the molecular response mechanisms of M. rosenbergii underlying Cu toxicity effects but also helps us to identify potential biomarkers associated with the stress response in other crustaceans.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Zhang,

Bao,

Guo

et al. 2024

Animals

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“…The activities of hepatopancreatic immune‐related enzymes are measured, and the immune‐related enzymes include catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) were measured using specific assay kits based on the manufacturer's protocol (Nanjing Jiancheng, China), two detoxification enzymes (CarEs and GSTs) in the hepatopancreas were measured as well. The method measured and activity calculation was performed according to the Zang, et al (Zang, Zhang, Huang, Ame, & Shen, 2020). Data were statistically analysed with SPSS v.18.0 using single‐factor ANOVA and Duncan's multiple range test, considering p < 0 .…”

Section: Methodsmentioning

confidence: 99%

Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Shen

Zhang

et al. 2020

Aquaculture Research

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To date, hepatopancreatic necrosis disease (HPND) in Eriocheir sinensis is causing huge financial losses to the aquaculture industry while the cause of HPND remains unclear. Our previous study has implied that HPND is probably relevant to the damages caused by insecticide residues. In this work, the comparative studies regarding histopathology and trends in immune‐related/detoxifying enzymes activities of the crabs were performed between exposed to the low concentration of insecticides (i.e. β‐cypermethrin 8.52 × 10−12 g/L and trichlorfon 5 × 10−5 g/L) in a long‐term (90‐days) and suffered from HPND. The concentration was obtained by a median‐lethal concentration (LC50) and a safe concentration (SC), and it was 10 times lower than the safe concentration in this experiment. This work's principal aim was to investigate the potential chronic effects of low concentrations of insecticides exposure on crabs and their relevance with HPND. As a result, crabs exposed to the low concentration of insecticides caused similar clinical symptoms and severity in hepatopancreas and other tissue damages like HPND. Meanwhile, catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM) and carboxylesterase (CarE) enzymatic activity increased after exposure to the two insecticides and then decreased in the later stages, while the activity of glutathione S‐transferase (GSTs) has barely changed. Results of enzymatic activity indicated that the two insecticides induced oxidative damage to the hepatopancreas of E. sinensis. These evidence indicated that the chronic damage caused by the low concentration of insecticides, which is considered insecticide residue, might be part of the causes of HPND in crabs. The present work may enhance our understanding of the pathogenesis of HPND and provide a theoretical basis for the management of intensive crab aquaculture.

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“…For instance, some authors report evident seasonal fluctuation in acetylcholinesterase (AChE) (e.g., Menezes et al, 2006 ), a key enzyme in cholinergic signal transmission in the sensory and neuromuscular systems that therefore appears to be likely related to animals’ locomotor activity ( Anita et al, 2017 ). Moreover, carboxylesterases (CbE), a multifunctional group of enzymes relevant in lipid synthesis and decomposition and signal transmembrane transduction ( Zang et al, 2020 ), are involved in the metabolism of endogenous compounds ( Nos et al, 2021 ), and thermal stress can compromise their capability to face environmental chemical stressors ( Saiz et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Moderate hypoxia mitigates the physiological effects of high temperature on the tropical blue crab Callinectes sapidus

García-Rueda

Mascaró²,

Rodrı́guez-Fuentes³

et al. 2023

Front. Physiol.

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Dissolved oxygen (DO) and water temperature vary in coastal environments. In tropical regions, the ability of aquatic ectotherms to cope with hypoxia and high-temperature interactive effects is fundamental for their survival. The mechanisms underlying both hypoxia and thermal tolerance are known to be interconnected, therefore, the idea of cross-tolerance between both environmental stressors has been put forward. We investigated the combined role of hypoxia and temperature changes on the physiological responses of blue crab Callinectes sapidus living in the southern Gulf of Mexico. We measured oxygen consumption, plasmatic biochemical indicators, total hemocyte count (THC), and antioxidant activity biomarkers in muscle and gill tissues of blue crab acclimated to moderate hypoxia or normoxia and exposed to a thermal fluctuation or a constant temperature, the former including a temperature beyond the optimum range. Animals recovered their routine metabolic rate (RMR) after experiencing thermal stress in normoxia, reflecting physiological plasticity to temperature changes. In hypoxia, the effect of increasing temperature was modulated as reflected in the RMR and plasmatic biochemical indicators concentration, and the THC did not suggest significant alterations in the health status. In both DO, the antioxidant defense system was active against oxidative (OX) damage to lipids and proteins. However, hypoxia was associated with an increase in the amelioration of OX damage. These results show that C. sapidus can modulate its thermal response in a stringent dependency with DO, supporting the idea of local acclimatization to tropical conditions, and providing insights into its potential as invasive species.

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Cloning and Expression of Two Carboxylesterases, and Their Activity Modulation in Chinese Mitten crab Eriocheir sinensis under Pesticide Exposer

Cited by 7 publications

References 10 publications

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Moderate hypoxia mitigates the physiological effects of high temperature on the tropical blue crab Callinectes sapidus

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