Cloning and expression of tonoplast membrane intrinsic protein genes in leaves of Vitis heyneana and overexpression of VhTIP2;1 in Arabidopsis confer drought tolerance

Wen-e, Zhang; Hu, Jingming; Li, Fēi; Chen, Erjuan; Zhao, Ting; Pan, Xuejun

doi:10.1007/s11738-023-03523-w

Cited by 7 publications

(1 citation statement)

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“…Studies have shown that some AQP genes can benefit plants under favorable and/or abiotic stress conditions [13,14], while others may have adverse effects [15,16]. However, our understanding of these proteins remains limited due to conflicting functions.…”

Section: Introductionmentioning

confidence: 99%

An Aquaporin Gene (KoPIP2;1) Isolated from Mangrove Plant Kandelia obovata Had Enhanced Cold Tolerance of Transgenic Arabidopsis thaliana

et al. 2023

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Aquaporins (AQPs) are essential channel proteins that play central roles in maintaining water homeostasis. Here, a novel aquaporin gene, named KoPIP2;1, was cloned from the mangrove plant Kandelia obovata by RACE technology. The KoPIP2;1 gene was 1404 bp in length with an open reading frame (ORF) of 852 bp, encoded with 283 amino acids. Database comparisons revealed that KoPIP2;1 protein shared the highest identity (91.26%) with the aquaporin HbPIP2;2, which was isolated from Hevea brasiliensis. Gene expression analysis revealed that the KoPIP2;1 gene was induced higher in leaves than in stems and roots of K. obovata under cold stress. Transient expression of KoPIP2;1 in Nicotiana benthamiana epidermal cells revealed that the KoPIP2;1 protein was localized to the plasma membrane. Overexpressing KoPIP2;1 in Arabidopsis significantly enhanced the lateral root number of the transgenic lines. KoPIP2;1 transgenic Arabidopsis demonstrated better growth, elevated proline content, increased superoxide dismutase (SOD) and peroxidase (POD) activities, and reduced malondialdehyde (MDA) content compared with the wild-type Arabidopsis when exposed to cold stress. The findings suggest that overexpression of KoPIP2;1 probably conferred cold tolerance of transgenic Arabidopsis by enhancing osmoregulation and antioxidant capacity. This present data presents a valuable gene resource that contributes to the advancement of our understanding of aquaporins and their potential application in enhancing plant stress tolerance.

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Section: Introductionmentioning

confidence: 99%

An Aquaporin Gene (KoPIP2;1) Isolated from Mangrove Plant Kandelia obovata Had Enhanced Cold Tolerance of Transgenic Arabidopsis thaliana

et al. 2023

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Exploring the role of HaTIPs genes in enhancing drought tolerance in sunflower

Safdar,

Tahir,

Ali

et al. 2023

Mol Biol Rep

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Background: Activity of plant aquaporins (AQPs) is extremely sensitive to environmental variables such as temperature, drought atmospheric vapor pressure de cit, cell water status and also appears to be closely associated to expressions of plant tolerance to various stresses. The spatial and temporal expression patterns of genes of Tonoplast Intrinsic Proteins (TIPs) in various crops indicates the complex and diverse regulation of these proteins and are important in understanding their key role in plant growth, development and stress responses.Methods and Results: Based on phylogenetic analysis, six distinct HaTIPs were selected for studying their spatial and temporal expression in sun ower (Helianthus annuus). In this study semi quantitative polymerase chain reaction (semi q-PCR) and real time polymerase chain reaction (q-PCR) analysis were used to study the spatial and temporal expression of HaTIPsin sun ower. The results indicated that all of HaTIPs showed differential expression speci c to both the tissues and the accessions. Moreover, the expression of all HaTIPs was higher in cross compared to the parents. Results of semi q-PCR and real time PCR indicated an upregulation of expression of HaTIP-RB7 and HaTIP7 in drought tolerant entries at 12 hours of 20 % polyethylene glycol (PEG) treatment compared to 0 hour. Conclusion: Hence these genes can be utilized as potential target in improving water use e ciency and for further genetic manipulation for the development of drought tolerant sun ower. This study may further contribute to our better understanding regarding the precise role of HaTIPsthrough their spatial and temporal expression analysis and their application in sun ower drought stress responses.

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