Cloning and expression of the porcine<i>obese</i>gene

Bidwell, Christopher A.; Ji, Shaoquan; Frank, Glenn; Cornelius, S. G.; Willis, Gawain M.; Spurlock, Michael E.

doi:10.1080/10495399709525882

Cited by 46 publications

(36 citation statements)

References 19 publications

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“…In livestock animals, the goal is still to identify the complete sequence of one of all genes carrying identified mutations responsible for the disease; the obese gene has recently been cloned for mice, human, and swine (Bidwell et al,1997).…”

Section: Introductionmentioning

confidence: 99%

Influence of obesity gene in quantitative traits of swine

Borges

Goulart

2002

Genet. Mol. Biol.

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Genotype data of 477 animals of several swine races (Landrace -LD, Large White -LW, Pietrain -PI, LWXLDXPI, Piau, Monteiro, and unknown race) were obtained to determine the allele frequency of the obesity gene. Genotype data of 174 crossbred swine (LWXLDXPI) were also obtained, in order to assess its correlation with carcass evaluation data (lean meat percentage, backfat thickness at P2, loin eye area, adjacent fat area, total fat and meat). Finally, genotype data of 96 pure swine (Landrace, Large White and Pietrain) were collected, to establish its relation with meat quality (drip loss, meat color, texture analysis and intramuscular fat) and carcass evaluation data (lean meat percentage; ham, loin, shoulder and belly weights; and backfat thickness at P2). This work also aimed associating EPDs (expected progeny differences) for litter size, daily weight gain and backfat thickness with genotype data of 49 Large White males and 54 Landrace females. Genotyping was done on animal blood by PCR-RFLP, based on Stratil et al. (1997). Statistical analysis was done by using SAS software for variance analysis between genotypes and data for each cited class. For purebred animals, a mixed model was used, with sire within race as random effect. The allelic frequencies of alleles T and C were, respectively: 0.8142; 0.1857 (Landrace); 0.9125; 0.0875 (Large White); 0.9433; 0.0566 (Pietrain); 0.8333; 0.1666 (LWXLDXPI); 0.2500; 0.7500 (Piau); 0.8750; 0.1250 (Monteiro), and 0.8870; 0.1130 (unknown race). Since the highest allele C frequency occurred in Piau, we suggest that this allele could be associated with fat accumulation. In the Landrace race, a study was done separating the frequencies of 2 generations (great-grandfather and grandfather), and the differences confirmed by a Chi-square test, a higher frequency of allele C having been found in the grandparental generation. This suggests that this allele could be eliminated by selection from the great-grandparental generation, when the male grandparent is replaced by the great-grandparental generation. The obesity gene did not influence any of the carcass evaluation data from crossbred animals. In pure swine, where the only genotypes were TT and TC, it greatly influenced shoulder weight and meat texture, with the highest average in heterozygotes (shoulder: 4.07 vs. 3.93; texture: 2.62 vs. 1.82), suggesting better carcass quality and worse meat quality than in homozygotes. The obesity gene did not influence any trait in the expected progeny difference (EPD) study.

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Section: Introductionmentioning

confidence: 99%

Influence of obesity gene in quantitative traits of swine

Borges

Goulart

2002

Genet. Mol. Biol.

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“…In pigs, the gene is expressed in the adipose tissue as 3.100bp mRNA (BIDWELL et al, 1997). A higher level of leptin mRNA was found in subcutaneous adipose tissue of finished pigs (136 kg) than that in those still growing (60 kg) (BIDWELL et al, 1997). By approximately 306% higher level of leptin mRNA and the protein alone was found in the plasma of obese pigs in relation to the non-obese pigs of the same body weight (RAMSAY et al, 1998).…”

Section: Introductionmentioning

confidence: 93%

“…The gene expression is regulated by adipose tissue mass and hormones, such as insulin, glucocorticoids (HOUSEKNECHT and PORTOCARRERO, 1998), estrogens (SHIMIZU et al, 1997), prolactin (GUALILLO et al, 1999), testosterone (BLACHE et al, 2000), and GH (HOUSEKNECHT et al, 2000). In pigs, the gene is expressed in the adipose tissue as 3.100bp mRNA (BIDWELL et al, 1997). A higher level of leptin mRNA was found in subcutaneous adipose tissue of finished pigs (136 kg) than that in those still growing (60 kg) (BIDWELL et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars

2003

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SummaryStudies on porcine genome contributed to identification of polymorphic loci of the genes that may influence the level of reproduction-related traits. The LEP gene codes for leptin -a protein secreted to blood from adipocytes, important for food intake, energy expenditure, and immune response; the protein also influences some functions of mammalian reproductive system. The aim of this study was to determine the polymorphism of HinfI in the leptin gene and its effect on selected qualitative and quantitative traits of the semen. The analyses included 54 AI boars, which had been derived from crossing Duroc with Pietrain breeds. The genotypes were established with PCR-RFLP. Two alleles LEP/HinfI were identified: LEP T (152 bp) and LEP C (68 and 84 bp) with the frequencies 0.713 and 0.287 respectively. The genotype frequencies were as follows:

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“…Leptin plays an essential role in processes related to energy consumption and the amount of ingested food, thereby influencing mass and body composition (Perez-Montarelo et al, 2012). In swine populations, the leptin gene is located on chromosome 18 and consists of 3 exons and 2 introns (Bidwell et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of leptin and leptin receptor gene expression in different swine breeds

et al. 2014

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ABSTRACT.Leptin is an important regulator of appetite, energy metabolism, and reproduction and is mainly synthesized in the adipocytes and then secreted into the bloodstream. The leptin receptor was classified as type I cytokine receptor due to its structural homology with IL-6 receptors and the signaling pathways in which they are both involved. The aim of our study is to comparatively assess the gene expression levels of leptin (lep) and leptin receptor (lepr) in different swine breeds specialized either in meat production (Duroc, Belgian Landrace, Large White, Synthetic Lines LS-345, and LSP-2000) or fat production (Mangalitsa) in order to correlate them with morphological and productivity characteristics. Additionally, lepr pattern of expression was evaluated comparatively between different tissue types in the Mangalitsa breed. Our results revealed high expression of the lep gene in Mangalitsa compared to those of all the other breeds, while for the lepr gene, average/medium levels were registered in Mangalitsa and increased pattern of expression was found in the synthetic lines LS-345 and LSP-2000. Regarding the comparative analysis of lepr gene expression in various tissues in the Mangalitsa breed, elevated levels were found in the liver and kidney, while the lowest expression was identified in the brain and muscles. Our results suggest that the 7140-7148 (2014) Mangalitsa population exhibits leptin resistance, which might be correlated with atypical morpho-productive characteristics for this breed, such as below-average prolificacy and a strong tendency to accumulate fat.

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Cloning and expression of the porcineobesegene

Cited by 46 publications

References 19 publications

Influence of obesity gene in quantitative traits of swine

Influence of obesity gene in quantitative traits of swine

Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars

Comparative study of leptin and leptin receptor gene expression in different swine breeds

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Cloning and expression of the porcineobesegene

Cited by 46 publications

References 19 publications

Influence of obesity gene in quantitative traits of swine

Influence of obesity gene in quantitative traits of swine

Preliminary results on associations between leptin gene (&lt;i&gt;LEP&lt;/i&gt;) and some reproduction performance traits of boars

Comparative study of leptin and leptin receptor gene expression in different swine breeds

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Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars