Cloning and expression of the cDNA for bovine granulocyte-macrophage colony-stimulating factor

Leong, Steven R.; Flaggs, G.; Lawman, M. J. P.; Gray, Patrick W.

doi:10.1016/0165-2427(89)90036-6

Cited by 23 publications

(8 citation statements)

References 35 publications

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“…The effect of hu.GM‐CSF on bone marrow cells of cattle or sheep is poor or even undetectable 47,48 . Likewise, the effect of hu.GM‐CSF on equine bone marrow cells is negligible 36 .…”

Section: Discussionmentioning

confidence: 99%

“…The effect of hu.GM-CSF on bone marrow cells of cattle or sheep is poor or even undetectable. 47,48 Likewise, the effect of hu.GM-CSF on equine bone marrow cells is negligible. 36 The residual cross-reactivity of hu.GM-CSF and eq.GM-CSF is most likely the result of the conservation of the protein in the regions from amino acids 21 to 31 and amino acids 78 to 94 (human numbers).…”

Section: Discussionmentioning

confidence: 99%

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Monocyte‐derived dendritic cells from horses differ from dendritic cells of humans and mice

2006

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SummaryDendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other species and rapid amplification of cDNA ends reverse transcription-polymerase chain reaction (RACE RT-PCR), the cDNA of equine (eq.) GM-CSF was cloned and found to have a point deletion at the 3 0 -end of eq.GM-CSF, resulting in a 24-nucleotide extended open reading frame not described in any species thus far. For differentiating eq.MoDC, monocytes were stimulated with eq.GM-CSF and eq.IL-4. The eq.MoDC was analysed by both light and electron microscopy and by flow cytometry and mixed lymphocyte reaction. The eq.MoDC obtained had the typical morphology and function of DC, including the ability to stimulate allogeneic T cells in a mixed lymphocyte reaction. In contrast to the human system, however, monocytes had to be differentiated for 6-7 days before immature DC were obtained. Our data also indicate that lipopolysaccharide or poly(I:C) alone are not sufficient to confer the full phenotypic transition into mature DC. Thus our study contributes to understanding the heterogeneity of immunity and adds important information on the equine immune system, which is clearly distinct from those of mice or man.

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“…The effect of hu.GM‐CSF on bone marrow cells of cattle or sheep is poor or even undetectable 47,48 . Likewise, the effect of hu.GM‐CSF on equine bone marrow cells is negligible 36 .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Monocyte‐derived dendritic cells from horses differ from dendritic cells of humans and mice

2006

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“…In comparison to the predicted molecular weight of bovine GM-CSF of 16,160 (12), the inferred ovine GM-CSF molecule has a molecular weight of 16,285 (including the signal sequence). The ovine nucleotide sequence shows an overall homoiogy of 91% with that of the bovine (12,13), excluding the terminal sections of the sequence where the PCR primers dictate that the sequence will conform to that of the bovine. Currently, studies are being undertaken using the PCR to determine whether the actual ovine nucleotide sequence at the 5' and 3' ends is identical to the bovine primers.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2 shows the alignment of the deduced amino acid sequence of ovine GM-CSF compared to GM-CSF of other species. The ovine and bovine sequences are 81% homologous (excluding the primer sequences), with no variations occurring in the first 17 amino acids which corresponds to a signal region (12,13). The four cysteine residues in the mature polypeptide are conserved throughout all the species, indicating similar secondary structures formed by disulfide bonding.…”

Section: Resultsmentioning

confidence: 99%

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Cloning and sequencing of the cDNA for ovine granulocyte‐macrophage colony‐stimulating factor (GM‐CSF)

O’Brien¹,

Rothel²,

Seow³

et al. 1991

Immunology & Cell Biology

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Summary Colony-stimulating factors (CSFs) are not only regulators of haemopoiesis but can also enhance the function of mature myeloid cells, and are therefore potential immune adjuvants. By use of the polymerase chain reaction (PCR) with primers based on the bovine granulocyte-macrophage CSF (GM-CSF) sequence, we have amplified the cDNA for ovine GM-CSF, produced from crude mRNA extracted from alveolar macrophages. The PCR product was cloned into pUC 119, and electroporated into Escherichia coli. The complete nucleotide sequence of two clones, and the partial sequence of eight others, was determined. At the nucleotide and amino acid levels, the ovine and bovine GM-CSF sequences are 91% and 81% homologous, respectively.

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Evaluation of human N-linked glycosylation sites in murine granulocyte-macrophage colony-stimulating factor

Altmann

Prystowsky

1992

Archives of Biochemistry and Biophysics

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Cloning and expression of the cDNA for bovine granulocyte-macrophage colony-stimulating factor

Cited by 23 publications

References 35 publications

Monocyte‐derived dendritic cells from horses differ from dendritic cells of humans and mice

Monocyte‐derived dendritic cells from horses differ from dendritic cells of humans and mice

Cloning and sequencing of the cDNA for ovine granulocyte‐macrophage colony‐stimulating factor (GM‐CSF)

Evaluation of human N-linked glycosylation sites in murine granulocyte-macrophage colony-stimulating factor

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