Cloning and expression of selenoprotein W from pearl mussels Cristaria plicata

Molecular cloning and sequence analysis of selenoprotein W gene and its mRNA expression patterns in response to metabolic status and cadmium exposure in goldfish, Carassius auratus

“…In human, the highest expression levels of SelW mRNA were in the muscle and heart (Schweizer et al, 2004;Whanger, 2001), whereas the predominant expression of chicken SelW was in the nervous tissue, muscle and gizzed, weak expression in testis, ovary, pancreas and kidney (Li et al, 2011). Recently, in mussels, the most abundance was in hepatopancreas, much higher than that of in muscle (Hu et al, 2014). In this study, the gfSelW mRNA widely expressed and was detected in all goldfish tissues examined.…”

“…Many reports had found that SelW mRNA was expressed in many tissues, including muscle, liver, heart, testis, spleen, kidney and brain (Hu et al, 2014;Schweizer et al, 2004;Whanger, 2001;Yeh et al, 1995Yeh et al, , 1997. However, the tissue expression pattern was not exactly the same among different species.…”

“…In mammals, the highest expression levels of SelW mRNA were in the muscle and heart (Schweizer et al, 2004;Whanger, 2001). However, in mussels the most abundance was in hepatopancreas, much higher than that of in muscle (Hu et al, 2014). The expression of SelW mRNA was strongly regulated by Se concentrations, and Se deficiency could lead to the decrease of SelW mRNA expression in many tissues (Pagmantidis et al, 2005;Sun et al, 1998;Whanger, 2009;Yeh et al, 1995Yeh et al, , 1997.…”

“…The expression of SelW mRNA was strongly regulated by Se concentrations, and Se deficiency could lead to the decrease of SelW mRNA expression in many tissues (Pagmantidis et al, 2005;Sun et al, 1998;Whanger, 2009;Yeh et al, 1995Yeh et al, , 1997. Moreover, after bacterial infection, the mRNA expression of SelW was significantly down-regulated in hemocytes, gill and hepatopancreas in mussels, suggesting its role in anti-microorganism innate immunity (Hu et al, 2014).…”

“…It was first reported as a missing component in Se-deficient animals suffering from white muscle disease (Whanger, 2009). Recently, SelW gene has been identified from human, rat, mouse, monkey, pig, sheep, chicken (Ou et al, 2011), zebrafish (Kryukov and Gladyshev, 2000) and pearl mussels (Hu et al, 2014). The SelWs obtained from the nine species contain 85-88 amino acids with the Sec residue at position 13 and had the characteristic signature sequence, CXXU.…”