Cloning and expression of porcine adiponectin and adiponectin receptor 1 and 2 genes in pigs1

Abstract

In mice, adiponectin receptors (AdipoR) have been found to mediate the effect of adiponectin in muscle and liver in regulation of glucose and fatty acid metabolism. The purposes of this study were to clone these receptors from pig tissues by reverse transcription PCR using mRNA from skeletal muscle and adipose tissue and to investigate the expression of these genes in various pig tissues. Sequences of adiponectin, AdipoR1, and AdipoR2 were determined and found to be highly homologous to those of the human and mouse. The AA sequences predicted for the full-length cDNA of porcine adiponectin, AdipoR1, and AdipoR2 were similar to those of the human and mouse, ranging from 81 to 97% homology, suggesting similar functions of these genes in pigs as in other species. Transcripts for adiponectin were abundant in s.c. adipose tissue in Lee-Sung pigs and in crossbred pigs. Transcripts for AdipoR1 were abundant in heart and skeletal muscle and also detected to a lesser extent (P < 0.05) in adipose tissue, liver, and spleen of the Lee-Sung pigs. Transcripts for AdipoR2 were abundant in s.c. adipose tissue and present to a lesser extent (P < 0.05) in the liver, heart, skeletal muscle, and spleen. These results indicate that the effect of adiponectin may be mediated through these receptors in various porcine tissues. Fasting for 8 h did not have a significant effect on the expression of adiponectin and AdipoR1 mRNA, but it increased (P < 0.05) the AdipoR2 mRNA in the s.c. adipose tissue of crossbred pigs. These results indicate that the AdipoR2-mediated fatty acid oxidation may be responsible at least in part for the fasted state fatty acid oxidation in porcine adipose tissues. The successful cloning of pig adiponectin and adiponectin receptors will enhance the understanding of the involvement of these genes in regulating energy metabolism in pigs.

Adiponectin action from head to toe

“…In response to fasting, mouse AdipoR1 mRNA is upregulated in brown adipose tissue and epigonadal fat and downregulated in subcutaneous fat, whereas AdipoR2 gene expression is only upregulated in epigonadal adipose tissue [40]. The modulation of gene expression can also be species-specific, as fasting in pigs had no effect on the expression of AdipoR1 but increased the AdipoR2 mRNA level in subcutaneous adipose tissue [41], whereas the gene expression of AdipoR1, but not of AdipoR2, was lower in the subcutaneous adipose tissue of fasted mice than in that of fed mice [40].…”

Serum Adiponectin Level as an Independent Predictor of Mortality in Patients With Congestive Heart Failure

“…13,14 Adiponectin can also inhibit growth factor-mediated fibroblast proliferation and prevent fibrosis. 15 Because these factors, namely, myocardial inflammation, hypertrophy and fibrosis, and dysregulation of myocardial lipid/glucose metabolisms can either cause or exacerbate CHF, 16,17 adiponectin may play a protective role in CHF.…”

Adiponectin receptors are expressed in hypothalamus and colocalized with proopiomelanocortin and neuropeptide Y in rodent arcuate neurons

“…It acts through two receptors: Adipor1 and Adipor2 (Yamauchi et al 2003. Adiponectin receptors were localized in various peripheral tissues (Kharroubi et al 2003, Berner et al 2004, Ding et al 2004, Kadowaki & Yamauchi 2005, Pineiro et al 2005, Dai et al 2006, Kadowaki et al 2008. However, Adipor1 is more abundantly expressed in muscles while Adipor2 predominates in the liver (Yamauchi et al 2003).…”