Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from “Tempoyak” Indonesian Fermented Food as Immunity Protein in Lactococcus lactis

Lages, Aksar Chair; Mustopa, Apon Zaenal; Sukmarini, Linda; Suharsono, _

doi:10.1007/s12010-015-1786-9

Cited by 12 publications

(8 citation statements)

References 36 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to adjusting the dose of nisin, several other ways can be used to increase the gene expression level, such as by substituting an inducible promoter to a constitutive promoter. In accordance to the results of Lages et al, 23 the administration of 10 ng/ml nisin induction in L. lactis increased gene expression, resulting in more protein than the non-induced. The concentrations of nisin has compared in the range between 0.5 and 5 ng/ml to induce the expression genes of antibacterial in L. lactis, and obtained the result that the highest of antibacterial was produced by L. lactis induced with nisin at 5 ng/ml by Mustopa et al 24 Moreover, codon optimization can also be performed.…”

Section: Discussionsupporting

confidence: 89%

The production of SPusp45-MSP-119 gene construct and its recombinant protein in Lactococcus lactis to be used as a malaria vaccine

et al. 2018

View full text Add to dashboard Cite

Background: Merozoite surface protein 1 (MSP-1) is a major protein used by the Plasmodium during red blood cells invasion in malaria. MSP-119, one of MSP-1 is highly conserved, and it is a potential malaria vaccine candidate because the monoclonal antibodies are capable blocking erythrocyte invasion in vitro. The aim of this study was to produce MSP-119 gene construct and the recombinant protein in Lactococcus lactis.Methods: Usp45-MSP-119, derived from codon optimization and the synthetic gene, was inserted into the pMAT cloning vector. A vector expressing MSP-119 included usp45 has been constructed by the manipulation of recombinant DNA using restriction enzymes. The MSP-119 protein was expressed to 45% ammonium sulfate precipitation and purified using Sephadex-G50 gel filtration chromatography. The expressed protein was characterized by SDS-PAGE and dot blot.Results: usp45-MSP-119 gene was amplified using specific primers and inserted into the multiple cloning sites in the expression vector pNZ8148 with size 3,538 bp as a recombinant vector. The protein of MSP-119 was successfully expressed in L. lactis with molecular weight of 10.45 kDa. The dot blot was tested in 3 different comparisons between the host cells, non-induced cells, and induced cells with 10 ng/ml nisin. The results showed that 10 ng/ml nisin gave a positive reaction as detected by dot blot assay.Conclusion: This study confirmed that the usp45-MSP-119 gene was successfully inserted into the multiple cloning sites of the pNZ8148 expression vector and the MSP-119 protein expressed in the NICE system of the L. lactis host cell.

show abstract

Section: Discussionsupporting

confidence: 89%

The production of SPusp45-MSP-119 gene construct and its recombinant protein in Lactococcus lactis to be used as a malaria vaccine

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This system has been used to produce high levels of recombinant Plantaricin W (Pln W) protein in L. lactis, and maximum expression of recombinant PlnW was obtained by induction with 50 ng/ml nisin (Lages et al, 2015). In the present research, induction with 10 ng/ml nisin ge-nerated the highest HBcAg protein expression, which was further confirmed by immunoblotting and concentration of the total protein fraction (Table 5 and Fig.…”

Section: Discussionsupporting

confidence: 78%

Construction and expression of indonesian hepatitis B core antigen (HBcAg) in Lactococcus lactis as potential therapeutic vaccine

Anwar¹,

Mustopa²,

Ningrum³

et al. 2019

bta

Self Cite

View full text Add to dashboard Cite

The hepatitis B core antigen protein (HBcAg) may induce proliferation of B, T, and cytotoxic T cells; induce immune responses; and provide T-cell resources for antibody responses after vaccination (Freivalds et al., 2011). Thus, HBcAg may be used as a therapeutic vaccine in patients with chronic hepatitis B virus (HBV) infection. This article describes a study on the construction and expression of HBcAg in Lactococcus lactis by the nisin-controlled expression (NICE) system. An HBcAg gene, the HBV subgenotype B3 that is dominant in Indonesia, was successfully cloned into a pNZ8148 vector and resulted in the formation of the transformant L. lactis NZ3900 pNZ818-HBcAg. The HBcAg protein measured 21 kDa. Induction with 10 ng/ml nisin significantly increased the concentration of the expressed protein. Western blotting and dot blot hybridization analysis indicated that the HBcAg proteins confirmed the expression of an antibody, with potential to become an HBV therapeutic vaccine.

show abstract

“…) and plantaricins (Lages et al . ), respectively. However, we did not specifically identify such factors in this investigation.…”

Section: Resultsmentioning

confidence: 95%

Antagonistic effects ofStreptococcusandLactobacillusprobiotics in pharyngeal biofilms

Humphreys

McBain

2019

Lett Appl Microbiol

View full text Add to dashboard Cite

Significance and Impact of the Study: Candidate probiotic bacteria deployed to prevent or treat bacterial pharyngitis will interact with the target bacteria such as Streptococcus pyogenes as well as with the microbiota of the throat, where off-target effects are possible. Three candidate probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Streptococcus salivarius reduced viability within extant S. pyogenes biofilms through the elaboration of diffusible factors other than fermentation acids but did not markedly disrupt ex situ pharyngeal microcosms. This work demonstrates the application of in vitro pharyngeal models in the preclinical testing of the safety and efficacy of candidate pharyngeal probiotics. AbstractDirect antagonism towards pathogens including Streptococcus pyogenes is a proposed mechanism of pharyngeal probiosis but off-target effects on the symbiotic microbiota of the throat are possible and may be beneficial, harmful or neutral. We have assessed the bacteriological effects of two candidate Lactobacillus probiotics and the established pharyngeal probiotic Streptococcus salivarius K12. Antagonism towards S. pyogenes and potential off-target effects were determined using sessile monospecies biofilms and pharyngeal microcosms, respectively. The candidate probiotics were antagonistic towards S. pyogenes (rank order of increasing potency, Lactobacillus acidophilus < Lactobacillus plantarum < Streptococcus salivarius) in the absence of significant acidification or cell-cell contact. Streptococcus salivarius and L. plantarum caused significant reductions in viable counts of streptococci in pharyngeal microbiotas, whilst S. salivarius also caused reductions in staphylococci. In contrast, changes in pharyngeal eubacterial DNA profiles were limited overall. In summary, the three candidate probiotics suppressed axenic Streptococcus pyogenes biofilms by mechanisms that did not depend on cell-cell contact or acidification and did not markedly destabilize complex pharyngeal microbiotas derived from healthy individuals.

show abstract

Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from “Tempoyak” Indonesian Fermented Food as Immunity Protein in Lactococcus lactis

Cited by 12 publications

References 36 publications

The production of SPusp45-MSP-1<sub>19</sub> gene construct and its recombinant protein in <em>Lactococcus lactis</em> to be used as a malaria vaccine

The production of SPusp45-MSP-1<sub>19</sub> gene construct and its recombinant protein in <em>Lactococcus lactis</em> to be used as a malaria vaccine

Construction and expression of indonesian hepatitis B core antigen (HBcAg) in Lactococcus lactis as potential therapeutic vaccine

Antagonistic effects ofStreptococcusandLactobacillusprobiotics in pharyngeal biofilms

Contact Info

Product

Resources

About