Cloning and expression of new microRNAs from zebrafish

Kloosterman, Wigard P.; Steiner, Florian; Berezikov, Eugène; Bruijn, Ewart de; Belt, José van de; Verheul, Mark; Cuppen, Edwin; Plasterk, Ronald H.A.

doi:10.1093/nar/gkl278

Cited by 164 publications

(136 citation statements)

References 44 publications

(71 reference statements)

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“…Analysis of multiple mouse tissues showed that miR 144 and miR 451 were most highly expressed in blood (Fig. 1B), consistent with previous reports that miR 451 is present in human erythrocytes (23) and in circulating blood of zebrafish (24). In mouse spleen, miR 144 and miR 451 were highly enriched in cells expressing Ter119, an erythroid-specific maturation marker (Fig.…”

Section: Resultssupporting

confidence: 90%

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A GATA-1-regulated microRNA locus essential for erythropoiesis

Doré

Amigo

Santos

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

299

310

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MicroRNAs (miRNAs) control tissue development, but their mechanism of regulation is not well understood. We used a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. Two conserved miRNAs, miR 144 and miR 451, emerged as direct targets of the critical hematopoietic transcription factor GATA-1. In vivo, GATA-1 binds a distal upstream regulatory element to activate RNA polymerase II-mediated transcription of a single common precursor RNA (pri-miRNA) encoding both mature miRNAs. Zebrafish embryos depleted of miR 451 by using antisense morpholinos form erythroid precursors, but their development into mature circulating red blood cells is strongly and specifically impaired. These results reveal a miRNA locus that is required for erythropoiesis and uncover a new regulatory axis through which GATA-1 controls this process.erythrocyte ͉ hematopoiesis ͉ transcription factor ͉ anemia ͉ gene expression

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Section: Resultssupporting

confidence: 90%

“…8A). Prior studies suggest that the two miRNAs are transcribed on a single precursor RNA (pri-miRNA) (12,(24)(25)(26). We verified this and localized the 5Ј end of the common pri-miRNA using RT-PCR and rapid amplification of cDNA ends (RACE) (SI Fig.…”

Section: Resultsmentioning

confidence: 58%

A GATA-1-regulated microRNA locus essential for erythropoiesis

Doré

Amigo

Santos

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

299

310

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“…This notion suggests that, although whole genome duplication can increase the diversity of miRNAs within a family, it does not lead to fundamental innovation or disparity, such as in the establishment of new miRNA families. In fact, where there is an unusually high rate of miRNA family acquisition, for example at the base of Nephrozoa (Table 1), there is no evidence for a GDE (11), and where there is a GDE, for example at the base of the teleost lineage (38), there is no evidence, despite extensive library searches (39), for an increase in the number of teleost-specific miRNA families. Indeed, in contrast to the rhetoric, no good evidence has been marshaled in support of the much-vaunted hypothesis that GDEs can confer increasing organismal complexity (10).…”

Section: Resultsmentioning

confidence: 99%

MicroRNAs and the advent of vertebrate morphological complexity

Heimberg

Sempere

Moy

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

370

339

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The causal basis of vertebrate complexity has been sought in genome duplication events (GDEs) that occurred during the emergence of vertebrates, but evidence beyond coincidence is wanting. MicroRNAs (miRNAs) have recently been identified as a viable causal factor in increasing organismal complexity through the action of these Ϸ22-nt noncoding RNAs in regulating gene expression. Because miRNAs are continuously being added to animalian genomes, and, once integrated into a gene regulatory network, are strongly conserved in primary sequence and rarely secondarily lost, their evolutionary history can be accurately reconstructed. Here, using a combination of Northern analyses and genomic searches, we show that 41 miRNA families evolved at the base of Vertebrata, as they are found and/or detected in lamprey, but not in either ascidians or amphioxus (or any other nonchordate taxon). When placed into temporal context, the rate of miRNA acquisition and the extent of phenotypic evolution are anomalously high early in vertebrate history, far outstripping any other episode in chordate evolution. The genomic position of miRNA paralogues in humans, together with gene trees incorporating lamprey orthologues, indicates that although GDEs can account for an increase in the diversity of miRNA family members, which occurred before the last common ancestor of all living vertebrates, GDEs cannot account for the origin of these novel families themselves. We hypothesize that lying behind the origin of vertebrate complexity is the dramatic expansion of the noncoding RNA inventory including miRNAs, rather than an increase in the protein-encoding inventory caused by GDEs.genome duplication ͉ lamprey ͉ macroevolution ͉ shark ͉ chordate

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“…Only the miRNAmiR-208 is known as heart-specific and plays an important role in the maintenance of heart development and function 15,16 . However, recent studies have shown that in long events such as heart injuries, this miRNA can flow into the bloodstream and be detected in peripheral blood.…”

Section: Micrornas and The Cardiovascular Systemmentioning

confidence: 99%

MicroRNAs: um novo paradigma no tratamento e diagnóstico da insuficiência cardíaca?

Oliveira-Carvalho¹,

Carvalho²,

Silva³

et al. 2012

Arq. Bras. Cardiol.

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MicroRNAs (miRNAs) are a group of newly discovered small RNAs, non-coding, which represent one of the most exciting areas of modern medical science as they modulate a huge and complex regulatory network of gene expression. Lines of evidence have recently suggested that miRNAs play a key role in the pathogenesis of heart failure. Some miRNAs highly expressed in the heart, such as miR-1, miR-133 and miR-208, are strongly associated with the development of cardiac hypertrophy, while the exact role of miR-21 in the cardiovascular system remains controversial. Serum levels of circulating miRNAs such as miR-423-5p are being evaluated as potential biomarkers in the diagnosis and prognosis of heart failure. On the other hand, the manipulation of levels of miRNAs using techniques such as mimicking the miRNAs (miRmimics) and antagonistic miRNAs (antagomiRs) is making increasingly evident the enormous potential of miRNAs as promising therapeutic strategies in heart failure.

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Cloning and expression of new microRNAs from zebrafish

Cited by 164 publications

References 44 publications

A GATA-1-regulated microRNA locus essential for erythropoiesis

A GATA-1-regulated microRNA locus essential for erythropoiesis

MicroRNAs and the advent of vertebrate morphological complexity

MicroRNAs: um novo paradigma no tratamento e diagnóstico da insuficiência cardíaca?

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