Cloning and expression of mRNA encoding prepro‐gonad‐inhibiting hormone (GIH) in the lobster <i>Homarus americanus</i>

Kleijn, Dominique P.V. de; Sleutels, Frank; Martens, Gerard J.M.; Herp, F. Van

doi:10.1016/0014-5793(94)01055-2

Cited by 81 publications

(56 citation statements)

References 17 publications

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“…For CHH-A, CHH-B and GIH, cDNA fragments (CHH-A, nucleotides 183-395; CHH-B, nucleotides 675-865 (De Kleijn et al 1995); GIH, nucleotides 527-850 (De Kleijn et al 1994)) were cloned into pBluescript and used as templates for specific cRNA probes. After synthesis and purification, each cRNA probe was dissolved in the protection-assay hybridization mix (80% formamide, 400 mM NaCl, 40 mM 1,4-piperazinediethanesulphonic acid, pH 6·4, 1 mM EDTA).…”

Section: Quantification Of Chh-a Chh-b and Gih Mrna Levels In The Eymentioning

confidence: 99%

“…Finally, when the presumed molt-inhibiting function of GIH (De Kleijn et al 1994) and the reported MIH effects of the hyperglycaemic hormone (Chang et al 1991) are taken into account, the lower blood levels of both peptides at late previtellogenesis may be responsible for induction of the molting process. Such a combined effect would be ideal for synchronization of the molt and reproductive cycles because molting is then inhibited in the immature ovary stages by GIH and in mature stages by the CHHs.…”

Section: Figurementioning

confidence: 99%

“…However, since GIH is also present in male lobsters (De Kleijn et al 1992), it must also have another role. After determination of the amino acid sequence of preproGIH and its alignment with the sequence of crab preproMIH, we found that not only do the mature hormones have a high degree of amino acid identity but the primary structures of their preprohormones are also nearly identical (De Kleijn et al 1994). Therefore we suggested that GIH may be an important modulator of synthesis or release of hormones involved in molting as well as the reproductive process (De Kleijn et al 1994).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Kleijn

Janssen²,

Waddy³

et al. 1998

Journal of Endocrinology

103

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Crustacean reproduction is regulated by a complex chain of hormonal interactions in which the crustacean hyperglycaemic hormones A and B (CHH-A and CHH-B) and the gonad-inhibiting hormone (GIH) play a primary role. These neurohormones are produced in the same neuroendocrine cells of the X-organ sinus gland complex, situated in the eyestalks of the American lobster, Homarus americanus. In order to obtain more information on the synthesis, storage, release and function of these three neuropeptides during the reproductive cycle, we studied the levels of their mRNAs in the X-organ, their peptide storage in the sinus gland and their concentration in the haemolymph at different stages of the female reproductive cycle. A high CHH-A mRNA level was found only in the previtellogenic stage, while elevated mRNA levels were determined for CHH-B in the mature as well as the previtellogenic stage. High CHH storage levels in the sinus gland were found during previtellogenesis. The total amount of CHH (CHH-A plus -B) in the haemolymph was significantly higher during maturation. A low level of GIH mRNA in the X-organ and a low amount of the GIH I isoform in the sinus gland were found only in the immature stage. In contrast, GIH haemolymph levels were high during the immature and previtellogenic stages. We conclude that CHH-A and -B are involved in triggering the onset of vitellogenesis and that CHH-B in particular is responsible for stimulating oocyte maturation before spawning, while GIH prevents the start of vitellogenesis in the ovary. Moreover, our results show that the balance between the haemolymph levels of the CHHs and GIH may tune the synchronization of reproduction and molting during the biannual reproductive cycle of the American lobster.

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Section: Quantification Of Chh-a Chh-b and Gih Mrna Levels In The Eymentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Kleijn

Janssen²,

Waddy³

et al. 1998

Journal of Endocrinology

103

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“…The preprohormones of the CHH subgroup always contain a second peptide, commonly referred to as CHH precursor-related peptide or CPRP, which is Cterminally flanked by the CHH (Weidemann et al, 1989;Tensen et al, 1991;de Kleijn et al, 1994a;Ohira et al, 1997a;Davey et al, 2000;Toullec et al, 2002;Marco et al, 2003;Chen et al, 2004;Mettulio et al, 2004;de la Iglesia et al, 2005;Hsu et al, 2005a;Toullec et al, 2006). The preprohormones of the MIH/GIH/VIH/MOIH sub-group lack precursor-related peptides (Klein et al, 1993;de Kleijn et al, 1994b;Sun, 1994;Lee et al, 1995;Ohira et al, 1997b;Chan et al, 1998;Gu and Chan, 1998;Umphrey et al, 1998;Tang et al, 1999;Lu et al, 2001;Yang and Rao, 2001;Edomi et al, 2002;Krungkasem et al, 2002;Ohira et al, 2005). Although CPRP has been shown to circulate in the hemolymph, the physiological role(s) it plays in crustaceans remains unknown (Wilcockson et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs ofCancercrabs: implications for differential release and pleiotropic function

Hsu

Messinger

Chung

et al. 2006

Journal of Experimental Biology

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Research on crustaceans has played a major role in advancing our understanding of neuroendocrine structure and control. Nearly 80 years ago, Koller (Koller, 1925;Koller, 1927) demonstrated that hemolymph-borne agents were responsible for color change in shrimp. In the decade that followed, Hanström (Hanström, 1931;Hanström, 1933;Hanström, 1935;Hanström, 1937) identified a structure located in the eyestalk, the sinus gland (SG) as the source of these substances. Later, Bliss (Bliss, 1951) and Passano (Passano, 1951) independently demonstrated the neurohemal nature of the SG, a first for any neural structure in the animal kingdom. In recent years, work from many laboratories has shown that the SG is one of the major neuroendocrine organs in crustaceans and a number of hormones contained within it have been identified, including a group of structurally related peptides that have collectively been termed the crustacean hyperglycemic hormone (CHH) family. The crustacean hyperglycemic hormone (CHH) family of peptides includes CHH, moult-inhibiting hormone (MIH) and mandibular organ-inhibiting hormone (MOIH). In the crabCancer pagurus, isoforms of these peptides, as well as CHH precursor-related peptide (CPRP), have been identified in the X-organ-sinus gland (XO-SG) system. Using peptides isolated from the C. pagurus SG, antibodies to each family member and CPRP were generated. These sera were then used to map the distributions and co-localization patterns of these peptides in the neuroendocrine organs of seven Cancer species: Cancer antennarius, Cancer anthonyi, Cancer borealis, Cancer gracilis, Cancer irroratus, Cancer magister and Cancer productus. In addition to the XO-SG, the pericardial organ (PO) and two other neuroendocrine sites contained within the stomatogastric nervous system, the anterior cardiac plexus (ACP) and the anterior commissural organ (ACO), were studied. In all species, the peptides were found to be differentially distributed between the neuroendocrine sites in conserved patterns: i.e. CHH, CPRP, MIH and MOIH in the XO-SG, CHH, CPRP and MOIH in the PO, and MOIH in the ACP (no immunolabeling was found in the ACO). Moreover, in C. productus (and probably in all species), the peptides present in the XO-SG and PO were differentially distributed between the neurons within each of these neuroendocrine organs (e.g. CHH and CPRP in one set of XO somata with MIH and MOIH co-localized in a different set of cell bodies). Taken collectively, the differential distributions of CHH family members and CPRP both between and within the neuroendocrine organs of crabs of the genus Cancer suggests that each of these peptides may be released into the circulatory system in response to varied, tissue-specific cues and that the POand/or ACP-derived isoforms may possess functions distinct from those classically ascribed to their release from the SG.

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“…5 The gene encoded this peptide was identified and found to be expressed only in the eyestalk. 6 In the Norway lobster, GIH mRNA was expressed mainly in male and female eyestalks but was also found in supraesophageal ganglia. 7 The characterization of GIH in other species are scarcely reported due in part to the difficulties in the purification and the bioassay of this hormone which is not feasible in most species of interest.…”

Section: Introductionmentioning

confidence: 99%

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Longyant¹,

Sithigorngul²,

Sithigorngul³

et al. 2003

ScienceAsia

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ABSTRACT:Competitive ELISA using a combination of monoclonal antibodies specific to vitellin subunits was used to monitor the fluctuation of haemolymph vitellogenin levels in Penaeus monodon during ovarian development induced by bilateral eyestalk-ablation and to monitor the effect of eyestalk extract injection in prawn with developing ovary. The haemolymph vitellogenin levels were undetectable in the prawn with ovary at the resting stage but elevated sharply when the ovary began to develop, remained high during the ovary developing into ripe stage then fell to the low levels before spawning and spent stages. The result from injection of eyestalk extract into prawn with developing ovary revealed that haemolymph vitellogenin levels elevated sharply within 2 hr, reached the maximal levels and remained high during 4-10 hr, then declined slightly at 24 hr. This response directly depended on the amount of injected eyestalk extract and the response was species specific. The application of eyestalk extract from Metapenaeus affinis demonstrated the same stimulatory effect but was less potent whereas the eyestalk extract from Macrobrachium rosenbergii did not cause any changes in haemolymph vitellogenin levels. Therefore, the assay is specific and provides an indicator to monitor the activity of the putative gonad inhibiting hormone by assaying the alteration of vitellogenin levels.

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Cloning and expression of mRNA encoding prepro‐gonad‐inhibiting hormone (GIH) in the lobster Homarus americanus

Cited by 81 publications

References 17 publications

Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs ofCancercrabs: implications for differential release and pleiotropic function

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