Cloning and expression of levansucrase from Leuconostoc mesenteroides B-512 FMC in Escherichia coli

Recent novel applications of levansucrases

“…However, the enzymes from G. stearothermophilus The enzyme produces levan (18 %), 1-kestose (17 %), nystose (11 %), 1,1,1-kestopentaose (7 %), and liberated glucose from 150 mM sucrose. Kang et al 2005 Leuconostoc mesenteroides NRRL B-512 F…”

Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512 F

“…In a later report, Miller and Robyt (1986) described two FTFs of 116 and 92 kDa but due to their low activity, their properties have not been studied. Recently, Kang et al (2005), have reported the isolation of a L. mesenteroides B-512 FMC levansucrase gene (m1ft). This gene codes for a protein of 424 amino acid residues with a calculated molecular mass of 47.1 kDa.…”

Enzymatic Synthesis and Characterization of Fructooligosaccharides and Novel Maltosylfructosides by Inulosucrase from Lactobacillus gasseri DSM 20604

“…This compound could be identified as inulobiose (1-␤-D-fructofuranosylfructose) by comparison with a honey sample where it had been previously identified (27). The presence of this peak could indicate that fructose could also act as a minor acceptor in the FTF reaction (18). The other compound was a trisaccharide that could be an isomer of 1-kestose, such as neo-kestose, according to its characteristic m/z ratios of 217/204 and 437/451.…”

“…In the case of glucansucrase enzymes, maltose is recognized as the best acceptor, providing the synthesis of a series of potentially bioactive oligosaccharide acceptor products such as panose (6-␣-D-glucopyranosylmaltose) and other isomaltooligosaccharides (15,16). When maltose acts as an acceptor in transfructosylation reactions with FTFs, only one acceptor product, named erlose [␣-D-glucopyranosyl-(1¡4)-␣-D-glucopyranosyl-(1¡2)-␤-D-fructofuranoside], produced using a levansucrase from different bacterial strains, such as Bacillus subtilis (17) and Leuconostoc mesenteroides, has been reported in the open literature to the best of our knowledge (18). This nonreducing trisaccharide, also known as maltosylfructose (17), glycosyl sucrose, or coupling sugar (19), is a carbohydrate that naturally occurs in honey (20,21).…”