Cloning and Expression ofSFRP5in Tibetan Chicken and its Relationship with IMF Deposition

Abstract

Secreted frizzled related protein 5 (SFRP5), an anti-inflammatory adipokine, is relevant to the adipocyte differentiation. In order to clarify its role in regulating intramuscular fat (IMF) deposition in Tibetan chicken, the full-length sequence of the Tibetan chicken SFRP5 gene was cloned. The relative expression of SFRP5 gene was detected using quantitative RT-PCR in various tissues of 154 days old Tibetan chicken, as well as in breast muscle, thigh muscle, and adipose tissue at different growth stages. The results showed that SFRP5 gene was expressed in all examined tissues but highly enriched in adipose tissue. Temporal expression profile showed that the expression of SFRP5 was gradually decreased in breast muscle, but was fluctuated in thigh muscle and adipose tissue with the growth of Tibetan chicken. Furthermore, correlation analysis demonstrated that the expression of SFRP5 in breast muscle, thigh muscle and adipose tissue was correlated with IMF content at different levels. The results indicated that Tibetan chicken SFRP5 is involved in IMF deposition.

The Wnt antagonist and secreted frizzled-related protein 5: implications on lipid metabolism, inflammation, and type 2 diabetes mellitus

“…Reports have revealed that SFRP5 initially binds with Wnt5a to prevent JNK activation in the downstream of Wnt signaling pathway in adipose tissues. As a result, we discovered a subsequent decrease in the production of pro-inflammatory cytokines, and antagonization of the IRS-1 Ser 307 phosphorylation [ 23 ]. Thus, SFRP5 has the ability to modulate the progression of T2DM as a result of its insulin-sensitizing and anti-inflammatory properties which have all been well documented [ 24 ].…”

“…The protective role of SFRP5 in the development of T2DM has been determined by findings that showed increased insulin sensitivity, decreased macrophage infiltration, and pro-inflammatory protein production [ 59 ]. The expression and transcription of SFRP5 have been reported to share a negative association with insulin resistance [ 23 ]. A reduction in SFRP5 transcription could result in an increase in the ratio of Wnt5a to SFRP5, which is correlated with inhibited glucose and insulin signaling [ 44 ].…”

Key genes and co-expression modules involved in asthma pathogenesis

“…However, WGCNA, being considered as a correlation analysis, cannot solve all problems, but needs to combine other appropriate methods. (Li et al, 2016).…”

“…Asthma is a complex disease with diverse underlying pathological mechanisms with both the young and the elderly (Hasegawa et al, 2017;Li et al, 2016;Lotvall et al, 2011). Bronchial hyperresponsiveness, airway remodeling (Movassagh et al, 2016), abnormal hormone secretion (Newton et al, 2017), and chronic airway inflammation (Parulekar, Diamant & Hanania, 2017) are some of the major clinical features of asthma.…”

“…These studies suggested that it is worthwhile to research the key genes and the pathogenic mechanisms of asthma. As such, one tool that can help researchers with the analysis of the relationships between key genes and the pathogenic mechanism is weight gene co-expression network analysis (WGCNA) (Li et al, 2016).…”