Cloning and expression of hyaluronate lyase genes ofStreptococcus intermediusandStreptococcus constellatussubsp.constellatus

Takao, Ayuko

doi:10.1016/s0378-1097(03)00023-5

Cited by 14 publications

(17 citation statements)

References 28 publications

(37 reference statements)

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“…Chondroitin sulfatase activity was also more prevalent in the SMG than has previously been reported (11,28) and was uniformly detected in S. intermedius and beta-hemolytic Lancefield group C S. constellatus isolates. These findings corroborate those found by Takao and colleagues (38,39), in that two types of hylauronidases exist within the SMG: those with chondroitin sulfatase activity, found only in S. intermedius and S. constellatus subsp. pharyngis, and those without chondroitin sulfatase activity, found in S. constellatus subsp.…”

Section: Discussionsupporting

confidence: 91%

Characterization ofStreptococcus milleriGroup Isolates from Expectorated Sputum of Adult Patients with Cystic Fibrosis

Grinwis¹,

Sibley²,

Parkins

et al. 2010

J Clin Microbiol

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With the recent insights into the Streptococcus milleri group (SMG) as pulmonary pathogens in patients with cystic fibrosis (CF), we sought to characterize 128 isolates from the sputum of adults with CF, along with 45 isolates from patients with invasive diseases for comparison. The tests performed included Lancefield grouping; tests for hemolysis; tests for the production of hyaluronidase, chondroitin sulfatase, DNase, proteases, and hydrogen peroxide; and PCR for the detection of the intermedilysin gene (ily). We also generated biochemical profiles with the Rapid ID Strep 32 API system and tested cell-free supernatants for the presence of the signal molecule autoinducer-2 (AI-2) using a Vibrio harveyi bioassay with a subset of CF strains. The S. intermedius isolates from both strain collections were similar, while the S. constellatus and S. anginosus isolates yielded several biotypes that differed in prevalence between the two strain collections. Beta-hemolytic, Lancefield group C S. constellatus comprised 74.4% of the S. constellatus isolates from patients with CF but only 13.3% of the corresponding isolates from patients with invasive infections. This was the only S. constellatus biotype associated with pulmonary exacerbations. Hyaluronidase-positive S. anginosus was detected only among the isolates from patients with CF. Strain-to-strain variability in AI-2 expression was evident, with the mean values being the highest for S. anginosus, followed by S. constellatus and then S. intermedius. Cluster analysis and 16S rRNA sequencing revealed that the species of SMG could be accurately determined with a minimum of three phenotypic tests: tests for the Lancefield group, hyaluronidase production, and chondroitin sulfatase production. Furthermore, isolates from patients with invasive infections clustered with isolates from the sputum of patients with CF, suggesting that the respiratory tract isolates were equally pathogenic. Streptococcus intermedius, Streptococcus anginosus, andStreptococcus constellatus are a group of organisms collectively referred to as the Streptococcus milleri group (SMG) or the S. anginosus group (8). Members of the SMG are generally considered to be commensals of the oral cavity, gastrointestinal tract, and female urogenital tract in 15 to 30% of healthy individuals (22) but are clinically known for their association with purulent infections throughout the body (3). Recently, members of the SMG were found to be responsible for up to half of invasive pyogenic streptococcal infections in a large Canadian health region (13) and, after Staphylococcus aureus, were the most common isolates in a microbiological analysis of skin and soft tissue infections (36). Members of the SMG are not widely recognized as pulmonary pathogens, but they have recently gained attention as such, particularly in those with cystic fibrosis (CF) (20, 32; C. D. Sibley, M. E. Grinwis, T. R. Field, M. D. Parkins, J. C. Norgaard, D. B. Gregson, H. R. Rabin, and M. G. Surette, submitted for publication). We have previously s...

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Section: Discussionsupporting

confidence: 91%

Characterization ofStreptococcus milleriGroup Isolates from Expectorated Sputum of Adult Patients with Cystic Fibrosis

Grinwis¹,

Sibley²,

Parkins

et al. 2010

J Clin Microbiol

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“…Here, we demonstrate that hyaluronate lyase is also required for growth of S. pneumoniae on hyaluronic acid. It remains to be determined if the hyaluronate lyases expressed by S. intermedius and S. aureus are also required for growth on hyaluronic acid (13,42).…”

Section: Discussionmentioning

confidence: 99%

Streptococcus pneumoniae Can Utilize Multiple Sources of Hyaluronic Acid for Growth

et al. 2012

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ABSTRACTThe mechanisms by whichStreptococcus pneumoniaeobtains carbohydrates for growth during airway colonization remain to be elucidated. The low concentration of free carbohydrates in the normal human airway suggests that pneumococci must utilize complex glycan structures for growth. The glycosaminoglycan hyaluronic acid is present on the apical surface of airway epithelial cells. As pneumococci express a hyaluronate lyase (Hyl) that cleaves hyaluronic acid into disaccharides, we hypothesized that during colonization pneumococci utilize the released carbohydrates for growth. Hyaluronic acid supported significant pneumococcal growth in anhyl-dependent manner. A phosphoenolpyruvate-dependent phosphotransferase system (PTS) and an unsaturated glucuronyl hydrolase (Ugl) encoded downstream ofhylare also essential for growth on hyaluronic acid. This genomic arrangement is present in several other organisms, suggesting conservation of the utilization mechanism between species.In vivoexperiments support the hypothesis thatS. pneumoniaeutilizes hyaluronic acid as a carbon source during colonization. We also demonstrate that pneumococci can utilize the hyaluronic acid capsule of other bacterial species for growth, suggesting an alternative carbohydrate source for pneumococcal growth. Together, these data support a novel function for pneumococcal degradation of hyaluronic acidin vivoand provide mechanistic details of growth on this glycosaminoglycan.

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“…Interestingly, the severity of gingivitis in young male adults correlated with their salivary hyaluronidase activity (Rovelstad et al, 1958). Among oral streptococci, only S. intermedius and Streptococcus constellatus produce hyaluronidase, with S. intermedius strains showing a higher frequency of enzymic activity (Takao, 2003). The S. intermedius hyaluronidase also breaks down chondroitin sulphate, although degradation rates are less than 8 % of the rate with HA (Shain et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The S. intermedius hyaluronidase also breaks down chondroitin sulphate, although degradation rates are less than 8 % of the rate with HA (Shain et al, 1996). The hyaluronidase in S. intermedius is closely related to that in Streptococcus pneumoniae, including conserved regions associated with carbohydrate binding, enzyme activity and cell wall anchoring domains (Takao, 2003). Most of the hyaluronidase activity is found in the culture supernatant (Homer et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Role of hyaluronidase in Streptococcus intermedius biofilm

2008

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Cloning and expression of hyaluronate lyase genes ofStreptococcus intermediusandStreptococcus constellatussubsp.constellatus

Cited by 14 publications

References 28 publications

Characterization ofStreptococcus milleriGroup Isolates from Expectorated Sputum of Adult Patients with Cystic Fibrosis

Characterization ofStreptococcus milleriGroup Isolates from Expectorated Sputum of Adult Patients with Cystic Fibrosis

Streptococcus pneumoniae Can Utilize Multiple Sources of Hyaluronic Acid for Growth

Role of hyaluronidase in Streptococcus intermedius biofilm

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