Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity

Wallner, Barbara; Mattaliano, Robert J.; Hession, Catherine; Cate, Richard L.; Tizard, Richard; Sinclair, L K; Foeller, Carl; Chow, E P; Browning, Jeffrey L.; Ramachandran, K L; Pepinsky, R. Blake

doi:10.1038/320077a0

Cited by 657 publications

(291 citation statements)

References 18 publications

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“…Although it is synthesized as a soluble cytoplasmic protein (Wallner et al, 1986), p35 is secreted (Christmas et al, 1991) and has extracellular anti-inflammatory and endocrine activities (Cirino et al, 1987;Pihoker et al, 1991). In rat embryos we have shown that p35 is expressed transiently at high levels in very restricted cell populations, a pattern characteristic of regulatory substances (McKanna and Cohen, 1989).…”

Section: Introductionmentioning

confidence: 73%

Optic chiasm and infundibular decussation sites in the developing rat diencephalon are defined by Glial Raphes expressing p35 (Lipocortin 1, Annexin I)

McKanna

1992

Developmental Dynamics

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ABSTRACTp35, a Ca+ +-phospholipid-binding protein that serves as a substrate for the EGF receptor tyrosine kinase, is expressed by primitive glial ependymal cells to define a raphe occupying the ventral midline in the spinal cord and hindbrain of rat embryos (McKanna and Cohen, Science 243:1477-1479). p35 appears transiently in the median one-third (80 pm) of the floor plate at precisely the time and place where axons cross to form the ventral commissure. We postulated that if p35 is involved with commissure development, homologous p35 raphes might be found at decussation sites rostral to the floor plate, including the optic chiasm. The present report describes two developmentally regulated p35 raphes in the diencephalon. One raphe is present for 2-3 days at the rostral lip of the nascent infundibulum, the reported decussation site of axons running from the supraoptic nucleus to the neurohypophysis; the other raphe appears in the rostral two-thirds of the optic chiasm, the site traversed by the optic axons. p35 is never expressed in the caudal one-third of the chiasm that accommodates non-retinal axons. To the best of our knowledge, this is the first identification of a specific marker for the retinal component of the optic chiasm. Because the p35 is gone by embryonic day 18.5, it is absent during final stages of chiasm formation when axons from the temporal retina decussate. Thus, p35 also may contribute to the "barrier" perceived by fibers that remain ipsilateral. Our data suggest that the p35 raphe contributes to the midline's role in commisure morphogenesis. Putative lipocortin activities including regulating PLA,, eicosanoids, or intracellular Ca+ + could be involved in altering cue specificity as decussating axon growth cones traverse the p35 compartment. o 1992 Wiley-Liss, Inc.

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Section: Introductionmentioning

confidence: 73%

Optic chiasm and infundibular decussation sites in the developing rat diencephalon are defined by Glial Raphes expressing p35 (Lipocortin 1, Annexin I)

McKanna

1992

Developmental Dynamics

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“…28 This effect takes two hours to develop. [29][30][31][32] It is possible that a cortisol depressant effect may have influenced the findings of Stanley et al, as at that sample time a neutrophilia was present in their patients. After 90 rain of anaesthesia and surgery, our patients did not show any alterations in total white counts compared with preoperative levels in any group probably due to a shorter duration of anaesthesia prior to the commencement of surgery in all groups.…”

Section: Discussionmentioning

confidence: 99%

Neutrophils from patients undergoing hip surgery exhibit enhanced movement under spinal anaesthesia compared with general anaesthesia

et al. 1992

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“…This hypothesis IS supported by the fact that hpocortm rnRNA Inductton has been detected m rat macrophages [44] and thymoeytes [21] after m vwo admmlstration of DEX. In addition.…”

Section: By Glucocortlcoldsmentioning

confidence: 93%

Dexamethasone induces the expression of the mRNA of lipocortin 1 and 2 and the release of lipocortin 1 and 5 in differentiated, but not undifferentiated U‐937 cells

Solito¹,

Raugei²,

Melli³

et al. 1991

FEBS Letters

101

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The effect of dexamcthasone on mRNA and protem synthesis of hpocortms (LCT) I, 2 and 5 has been investigated m W-937 cells A constltutwe expressIon of both mRNAs and protems was detected m undlfferentlated U-937 cells This constltutlve level was Increased time-and doscdependently by mcubatlon with phorbol myrlstate acetate (PMA) In U-937 cells dlffercntmted by 24 h mcubatlon with 6 n&ml PMA, dexdmethasone (DEX) (1 J!M for 16 h) caused an increased synthesis of the mRNA level of LCT-I and 2, but not of LCT-5, over the level Induced by PMA DEX had no effect m undlfferentlated cells Moreover, DEX stimulated the extracellular release of LCT-1 and 5, but not of LCT-2. and InhIbIted the release of PGEz and TXBZ only m the dlfferentlated U-937 cells These results suggest that the responsiveness of these cells to glucocoetlcolds IS dependent on the phase of cell dd-ferentlatlon The sckctwe release of hpocortms by dlfferentmtcd U-937 cells may explain, at least m part, the mhrbltlon by DEX of the prostanoid re!ease Llpocortm expresslon. Cell dlfferentlatlon, Dexamethasone, Elcosanold mhlbltlon INTRODUCTIONLqmcortms have been defined as protems mduced by glucocortrcolds through a receptor-dependent mechanism which are able to inhibit phosphohpase A, enzyme activity [l]. This mhlbitlon results in. (I) a decreased release of pro-inflammatory hpld mctabohtes like prostaglandins, leukotrlenes and platelet-activating factor, (II) an anti-inflammatory action which munlcks the effect of glucocortlcolds (revtewed by Flower [2]) L~po-cortins belong to a large family of cafclum-and phosphohpid-bmdmg proteins which have been differently named.annexins. calpactins, chromobmdms, ancharms, cafcimcdins, endonexms, calefectrms, lnhlbltor of blood coagufdtlon, proteins I-III (for review see [3,4]) At the present, eight flpocortms have been identified and sequenced They share a common fourfold repeat structure [5] and are characterized by a 17-ammo acid consensus sequence which may be impflcated m the bmdmg to biomembranes [6].Llpocortms In this paper we have tegted the hypothesis that the mduction of tfle proteus by gfucoco~ trcolds is dcpcndVolume 291, number 2 FEBS LETTERSOctober 199 I

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Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity

Cited by 657 publications

References 18 publications

Optic chiasm and infundibular decussation sites in the developing rat diencephalon are defined by Glial Raphes expressing p35 (Lipocortin 1, Annexin I)

Optic chiasm and infundibular decussation sites in the developing rat diencephalon are defined by Glial Raphes expressing p35 (Lipocortin 1, Annexin I)

Neutrophils from patients undergoing hip surgery exhibit enhanced movement under spinal anaesthesia compared with general anaesthesia

Dexamethasone induces the expression of the mRNA of lipocortin 1 and 2 and the release of lipocortin 1 and 5 in differentiated, but not undifferentiated U‐937 cells

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