Cloning and expression of AQP3, a water channel from the medullary collecting duct of rat kidney.

Echevarría, Miriam; Windhager, Erich E.; Tate, Suresh S.; Frindt, Gustavo

doi:10.1073/pnas.91.23.10997

Cited by 260 publications

(172 citation statements)

References 18 publications

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“…3 and Table 2). For example, we observed the upregulation of three genes whose elevated levels may disrupt normal glucose oxidation: the plasma membrane lactate transporter MCT (monocarboxylate transporter) 2 (Slc16a7) (1) and aquaporins 3 and 7 (14,23) (Table 2). Conversely, mitochondrial NADH dehydrogenase subcomplex 5 and the mitochondrial aspartate/glutamate exchanger were progressively suppressed by PPAR␥ overexpression and/or activation ( Table 2), changes that may decrease the production of mitochondrial coupling factors.…”

Section: Discussionmentioning

confidence: 99%

Impact of PPARγ overexpression and activation on pancreatic islet gene expression profile analyzed with oligonucleotide microarrays

Parton¹,

Diraison²,

Neill³

et al. 2004

American Journal of Physiology-Endocrinology and Metabolism

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. Impact of PPAR␥ overexpression and activation on pancreatic islet gene expression profile analyzed with oligonucleotide microarrays. Am J Physiol Endocrinol Metab 287: E390 -E404, 2004. First published May 4, 2004 10.1152/ajpendo.00016.2004.-Peroxisome proliferator-activated receptor-␥ (PPAR␥) serves as a target for the thiazolidinedione class of antidiabetic drugs and is an important regulator of adipose tissue differentiation. By contrast, the principal target genes for PPAR␥ in the pancreatic islet and the impact of their induction on insulin secretion are largely undefined. Here, we show that mRNAs encoding both isoforms of rodent PPAR␥, ␥1 and ␥2, are expressed in primary rat islets and are upregulated by overexpresssion of the lipogenic transcription factor sterol response element-binding protein 1c. Unexpectedly, however, oligonucleotide microarray analysis demonstrates that graded activation of PPAR␥ achieved with 1) the thiazolidinedione GW-347845, 2) transduction with adenoviral PPAR␥1, or 3) a combination of both treatments progressively enhances the expression of genes involved in fatty acid oxidation and transport. Moreover, maximal activation of PPAR␥1 reduces islet triglyceride levels and enhances the oxidation of exogenous palmitate while decreasing glucose oxidation, cellular ATP content, and glucose-, but not depolarization-stimulated, insulin secretion. We conclude that, in the context of the pancreatic islet, the principal response to PPAR␥ expression and activation is the activation of genes involved in the disposal, rather than the synthesis, of fatty acids. Although fatty acid oxidation may have beneficial effects on ␤-cell function in the longer term by countering ␤-cell "lipotoxicity," the acute response to this metabolic shift is a marked inhibition of insulin secretion.␤-cell; insulin; secretion; peroxisome proliferator-activated receptor-␥; sterol regulatory element-binding protein-1c; thiazolidinedione; glucolipotoxicity GROWING EVIDENCE SUGGESTS that ␤-cell secretory failure in some forms of type 2 diabetes is correlated with the accumulation of triglyceride (TG) within the pancreatic islet (28). In the Zucker diabetic fatty (ZDF) rat, overabundance of islet lipid is associated with impaired glucose-stimulated insulin secretion and decreased expression of the glucose transporter GLUT2/Slc2a2 (25), as well as enhanced nitric oxide formation (42) and apoptosis (44). Although the mechanisms underlying these changes, collectively termed "lipotoxicity" or "glucolipotoxicity" (36) are not fully elucidated, elevated levels of "lipogenic" transcription factors, such as sterol response element-binding protein-1c (SREBP-1c) (26) and peroxisome proliferator-activated receptor-␥ (PPAR␥) (26, 28), may play a role.PPAR␥, a member of the nuclear hormone receptor family, is translated from an alternately spliced mRNA in humans and rodents, generating three (␥1, ␥2, and ␥3) or two (␥1 and ␥2) isoforms, respectively, in these species (see Fig. 1A) (16,17,45,48). Whereas PPAR␥1 is present in many m...

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Section: Discussionmentioning

confidence: 99%

Impact of PPARγ overexpression and activation on pancreatic islet gene expression profile analyzed with oligonucleotide microarrays

Parton¹,

Diraison²,

Neill³

et al. 2004

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

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“…Briefly, AQP3 cDNA was cloned from rat kidney cDNA by polymerase chain reaction (PCR); the sense strand was 5'-CGGGATCCCATGGGTCGACAGAAGGAGTT-3', and the antisense strand was 5'-GCTCTAGAGGGTTTTATGGGGT-GTCC-3' (underlined sequences indicate inserted BamHI and XbaI sites, respectively). These primers were derived from the rat AQP3 sequence [10] (GenBank TM accession No. L35108).…”

Section: Preparation Of Aqp3 Crnamentioning

confidence: 99%

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

Yamaji

Seki

Matsukawa

et al. 2011

Journal of Reproduction and Development

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Abstract. Previously, we showed that the exogenous expression of aquaporin 3 (AQP3), an aquaglyceroporin, improved the tolerance of mouse oocytes to vitrification with a glycerol-based solution. In the present study, we examined conditions suitable for the expression of AQP3 and the ability of vitrified oocytes to develop in vitro and in vivo after fertilization. After only partial remove of cumulus cells, immature mouse oocytes (germinal vesicle stage) were injected with 5, 10 or 20 pg of AQP3 cRNA and cultured for 12 h for maturation. When matured oocytes were vitrified with a glycerol-based solution, 57-61% survived regardless of the amount of cRNA injected (5-20 pg). By contrast, no oocytes injected with water (control) survived. When the zona pellucida was removed from the vitrified oocytes and the oocytes were then fertilized in vitro and cultured, the proportions that were fertilized and developed into blastocysts were higher when the amount of cRNA injected was 5 pg than 10-20 pg. When 16 blastocysts were transferred to a pseudopregnant mouse, 5 developed to term, demonstrating that oocytes vitrified after injection of AQP3 cRNA retained the ability to develop to term. The water-permeability of cRNA-injected oocytes was higher than that of control oocytes from the maturing stage to the 1-cell zygote stage, whereas glycerol-permeability was higher only at metaphase II. This indicates that AQP3 was expressed for a relatively short period of time. These results suggest that the transient expression of water/cryoprotectant channels is effective for cryopreserving cells that have low membrane-permeability, such as mammalian oocytes. Key words: Aquaporin, Development, Membrane-permeability, Oocyte, Vitrification (J. Reprod. Dev. 57: [403][404][405][406][407][408] 2011) he permeability of the plasma membrane to water and cryoprotectants is one of the most important factors for successful cryopreservation of oocytes/embryos because it influences the formation of intracellular ice, the toxicity of cryoprotectants and osmotic swelling [1]. For cryopreservation of oocytes/embryos, vitrification has advantages over slow freezing in terms of the simplicity of the method and viability of the cells. As the solution used for vitrification contains a high concentration of cryoprotectants, it is much more toxic than the solution for slow-freezing. Therefore, the permeability of the plasma membrane would affect the survival of cryopreserved cells more in vitrification than in slow freezing. If the permeability is low, insufficient exposure to the cryoprotectants causes formation of intracellular ice. However, excessive exposure results in toxic effects of the cryoprotectants. Therefore, high permeability to water and cryoprotectants would be preferable for vitrification.We showed that a simple one-step method is effective for vitrification of mouse morulae [2] but that a two-step method with a pretreatment with a lower concentration of the cryoprotectant is favorable for vitrifying mouse embryos at early cleavage stages...

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“…Although several studies reported that AQPs may increase membrane permeability to glycerol (Stroud et al, 2003), urea (Echevarria et al, 1994), and perhaps CO 2 (Uehlein et al, 2003) or even ions (Yasui et al, 1999;Hazama et al, 2002), it is now well established that most of these transmembrane proteins increase the water permeability of cellular membranes. Thus, AQPs are potentially playing a role in many physiological processes (Maurel et al, 2002;Tyerman et al, 2002;Luu and Maurel, 2005) involving water flow through membranes.…”

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confidence: 99%

Water Transport by Aquaporins in the Extant Plant Physcomitrella patens

et al. 2008

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Although aquaporins (AQPs) have been shown to increase membrane water permeability in many cell types, the physiological role of this increase was not always obvious. In this report, we provide evidence that in the leafy stage of development (gametophore) of the moss Physcomitrella patens, AQPs help to replenish more rapidly the cell water that is lost by transpiration, at least if some water is in the direct vicinity of the moss plant. Three AQP genes were cloned in P. patens: PIP2;1, PIP2;2, and PIP2;3. The water permeability of the membrane was measured in protoplasts from leaves and protonema. A significant decrease was measured in protoplasts from leaves and protonema of PIP2;1 or PIP2;2 knockouts but not the PIP2;3 knockout. No phenotype was observed when knockout plants were grown in closed petri dishes with ample water supply. Gametophores isolated from the wild type and the pip2;3 mutant were not sensitive to moderate water stress, but pip2;1 or pip2;2 gametophores expressed a water stress phenotype. The knockout mutant leaves were more bent and twisted, apparently suffering from an important loss of cellular water. We propose a model to explain how the AQPs PIP2;1 and PIP2;2 delay leaf dessication in a drying atmosphere. We suggest that in ancestral land plants, some 400 million years ago, APQs were already used to facilitate the absorption of water.

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Cloning and expression of AQP3, a water channel from the medullary collecting duct of rat kidney.

Abstract: The einal part of the inner medullary cleing duct exhibits a high degree ofwater permeability that is independent of increased intracellular cAMP and not accounted for by the activity of the known renal epithelial water channels CH1P28

Cited by 260 publications

References 18 publications

Impact of PPARγ overexpression and activation on pancreatic islet gene expression profile analyzed with oligonucleotide microarrays

Impact of PPARγ overexpression and activation on pancreatic islet gene expression profile analyzed with oligonucleotide microarrays

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

Water Transport by Aquaporins in the Extant Plant Physcomitrella patens

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