Cloning and expression of a trypomastigote-specific 85-kilodalton surface antigen gene from Trypanosoma cruzi

Takle, Garry B.; Young, Ahn Na; Snary, David; Hudson, L.; Nicholls, Stephen C.

doi:10.1016/0166-6851(89)90102-3

Cited by 27 publications

(16 citation statements)

References 20 publications

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“…Other T. cruzi 85-kD surface antigen coding genes have been cloned, and like the SA85-1 genes, they belong to large gene families (19)(20)(21) . These cloned genes do not share significant homology with each other or with SA85-1, indicating that multiple 85-kD families exist, which would further explain the observed surface antigen diversity.…”

Section: Gcg]dggagtgtgcggcctcagtgtggagtatttgcgccctcttccacaccgacactcacmentioning

confidence: 99%

“…Several trypomastigote surface antigens have been identified, including those with molecular masses of 90 kD (17), 160 kD (18), and the major 85 kD (9,19,20). A gene encoding an 85-kD surface antigen has been shown to be telomeric (21), and has given rise to speculation about the possibility of antigenic variation in T. cmzi, similar to variant surface glycoprotein (VSG)t switching in African trypanosomes (21).…”

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confidence: 99%

“…2) and no significant homology was detected between this sequence and those of previously described T . cruzi genes encoding 85-kD surface antigens (19)(20)(21). Thus, the 1.1 cDNA clone defines a new T .…”

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confidence: 99%

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The major 85-kD surface antigen of the mammalian form of Trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes.

Kahn

Voorhis

Eisen

1990

The Journal of Experimental Medicine

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SummaryTrypanosoma cruzi is an obligate intracellular protozoan parasite. The parasite mammalian stage surface antigens exhibit extensive antigenic diversity. We have characterized a family of T. cruzi genes that code for a polymorphic set of85-kD surface antigens, the SA85-1 antigens. The family contains >100 genes and pseudogenes, of which a minimum of nine are transcribed. The gene family is expressed in the mammalian stage only. A subset of the gene family is present in two Teomere-linked copies in the genome . Telomere linkage of other expressed SA85-1 genes has not been demonstrated. We have shown that at least three members of the SA85-1 gene family encode antigens at the surface of the mammalian stage of the parasite. Interestingly, these three antigens are expressed on all the trypanosomes examined. This suggests that T. cmzi simultaneously expresses a large repertoire of similar, but diverse antigens at its surface. Thus, T. cruzi exhibits extensive antigenic diversity in a system unique from that ofAfrican trypanosomes, perhaps reflecting its intracellular niche. rypanosoma cmzi, an obligate intracellular protozoan para-

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Section: Gcg]dggagtgtgcggcctcagtgtggagtatttgcgccctcttccacaccgacactcacmentioning

confidence: 99%

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confidence: 99%

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The major 85-kD surface antigen of the mammalian form of Trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes.

Kahn

Voorhis

Eisen

1990

The Journal of Experimental Medicine

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“…The major surface glycoproteins of the bloodstream trypomastigote stage of Trypanosoma cruzi are encoded by a large multigene family [1][2][3][4][5][6][7][8][9][10], several members of which are expressed simultaneously and encode glycoproteins of 85-160 kDa. We have previously isolated, characterized, and obtained the complete nucleotide sequence of TSA-1, a surface antigen gene from the Peru strain of T. cruzi belonging to the 85-kDa multigene family [1,2,5].…”

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“…However, the aa sequences downstream of the repeat units in TSA-1 are absent in TSA-S1 with the exception of the 21 amino acid residues immediately upstream of the translational stop site. The COOH terminus of the coding region represents one of the two regions conserved in all 85-kDa multigene family members characterized to date [2][3][4][5][6][7][8] and is the proposed processing site for a phosphatidylinositol linkage [12]. The other conserved region found in all family members examined is a 12-aa region which lies immediately upsteam of the repeat array in TSA-S1 [4,10].…”

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Isolation and characterization of the TSA/trans-sialidase family gene from the Silvio strain of Trypanosoma cruzi

Ruef¹,

Manning²

1993

Molecular and Biochemical Parasitology

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Only some members of a gene family in Trypanosoma cruzi encode proteins that express both trans-sialidase and neuraminidase activities.

et al. 1992

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Trypomastigotes, the blood stage form of the human parasite Trypanosoma cruzi, contain an enzyme on their surface, trans‐sialidase, which catalyses the transfer of sialic acid from host glycoconjugates to acceptors on its own cell surface. At least a subset of the sialic acid‐bearing acceptor molecules are involved in parasite invasion of host cells, an essential step in the life cycle of the parasite. Another trypomastigote surface enzyme that affects host cell invasion is neuraminidase and recent evidence suggests that both trans‐sialidase and neuraminidase activities may be expressed by the same proteins on the parasite surface. We describe here the isolation and expression of several members of a trans‐sialidase‐‐neuraminidase gene family from T.cruzi. One of the isolated genes does indeed encode a protein with both trans‐sialidase and neuraminidase activities, while other members of the gene family encode closely related proteins that express neither enzymatic activity. Chimeric protein constructs combining different portions of active and inactive genes identified a region of the gene necessary for enzymatic activity. Sequence analysis of this portion of the gene revealed a limited number of amino acid differences between the predicted active and inactive gene products.

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Cloning and expression of a trypomastigote-specific 85-kilodalton surface antigen gene from Trypanosoma cruzi

Cited by 27 publications

References 20 publications

The major 85-kD surface antigen of the mammalian form of Trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes.

The major 85-kD surface antigen of the mammalian form of Trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes.

Isolation and characterization of the TSA/trans-sialidase family gene from the Silvio strain of Trypanosoma cruzi

Only some members of a gene family in Trypanosoma cruzi encode proteins that express both trans-sialidase and neuraminidase activities.

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