Cloning and Expression Analysis of a Novel Mesodermally Expressed Cadherin

Abstract

Cadherins are calcium-dependent cell adhesion molecules which show developmental and tissue-specific expression. Here we report the cloning of a mouse cadherin which is predominantly expressed in tissues of mesodermal origin. In contrast to other cadherins, cadherin-11 expression is largely restricted to mesenchymal tissues surrounding various organs but is not found in epithelia. Sequence analysis suggests that this cadherin is the mouse homologue of the previously reported human cadherin-11 and is a member of a cadherin subfamily, which is evolutionarily distinct from other cadherin subfamilies identified so far.

Coexpression of two mesenchymal cadherins, cadherin 11 and N‐cadherin, on murine fibroblast‐like synoviocytes

“…Cadherin 11, a type II classic cadherin, was initially identified in osteoblast cell lines and was subsequently identified in neural tissue, lung, and kidney (18,19). Expression of cadherin 11 is associated with a more invasive phenotype in breast and prostate cancer cell lines as well as FLS (6,14,20,21).…”

Processus and recessus adhaerentes: giant adherens cell junction systems connect and attract human mesenchymal stem cells

“…The molecular composition of these junctions is simple. Apparently, the coexistence of N-cadherin and cadherin-11 is typical for puncta adhaerentia of diverse mesenchymal cells and tissues (e.g., Okazaki et al 1994;Hoffmann and Balling 1995;Kimura et al 1995;Simonneau and Thiery 1998;Hinz et al 2004), as are cytoplasmic plaques comprising α-and β-catenin, together with protein p120 and afadin, whereas appreciable amounts of other plaque proteins seem to be lacking (for references, see Straub et al 2003;Godsel et al 2004;Irie et al 2004; for a possible role of gelsolin in the anchorage of the actin filaments at adhering junctions of fibroblasts and probably also other mesenchymally derived cells, see Chan et al 2004). The functional importance of the molecular complement of these puncta adhaerentia in the differentiation of mesenchymal cells and tissue formation is indicated by the developmental defects described for mice lacking N-cadherin or cadherin-11 or both (Radice et al 1997;Horikawa et al 1999;Luo et al 2004;Kostetskii et al 2005) and by the general importance of cadherin-11 in the regulation of cell motility and cell-cell interaction in the dynamics of mesenchymal cells (cf.…”

Expression of cadherin-11 delineates boundaries, neuromeres, and nuclei in the developing mouse brain

“…This cadherin was found to be expressed strongly in various mesenchymal tissues in association with their morphogenesis, suggesting that it is involved in mesenchymal organization (Okazaki et al, 1994;Hoffmann and Balling, 1995;Kimura et al, 1995;Simonneau et al, 1995). In the present study, we investigated cadl 1 expression in the central nervous system during the development of the mouse embryo, focusing on the brain (more specifically, the forebrain).…”

“…We and others (Hoffmann and Balling, 1995;Simonneau et al, 1995) recently described the pattern of the expression of the mouse or rat homologue of cadherin-11 (cadll or OB-cadherin), a type-I1 cadherin, in embryos. This cadherin was found to be expressed strongly in various mesenchymal tissues in association with their morphogenesis, suggesting that it is involved in mesenchymal organization (Okazaki et al, 1994;Hoffmann and Balling, 1995;Kimura et al, 1995;Simonneau et al, 1995).…”