Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica

Wu, Renhua; Wang, Lili; Wang, Zhi; Shāng, Hǎihóng; Liu, Xia; Zhu, Yan; Qi, D.L.; Deng, Xin

doi:10.1016/j.pnsc.2008.07.010

Cited by 47 publications

(43 citation statements)

References 16 publications

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“…Similar to our results, rice OsDIRs exhibit greater response to salt stress as compared with mock-treated control seedlings [73]. Wu et al (2009) [74] also reported similar finding during their studies on dirigent protein gene from the resurrection plant Boea hygrometrica . In current study, we also performed the detached leaf assay of the CaChiIV1 -silenced gene with control plants treated with salt stress.…”

Section: Discussionsupporting

confidence: 89%

Classification and Genome-Wide Analysis of Chitin-Binding Proteins Gene Family in Pepper (Capsicum annuum L.) and Transcriptional Regulation to Phytophthora capsici, Abiotic Stresses and Hormonal Applications

Ali

Luo

Khan

et al. 2018

IJMS

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Chitin-binding proteins are pathogenesis-related gene family, which play a key role in the defense response of plants. However, thus far, little is known about the chitin-binding family genes in pepper (Capsicum annuum L.). In current study, 16 putative chitin genes (CaChi) were retrieved from the latest pepper genome database, and were classified into four distinct classes (I, III, IV and VI) based on their sequence structure and domain architectures. Furthermore, the structure of gene, genome location, gene duplication and phylogenetic relationship were examined to clarify a comprehensive background of the CaChi genes in pepper. The tissue-specific expression analysis of the CaChi showed the highest transcript levels in seed followed by stem, flower, leaf and root, whereas the lowest transcript levels were noted in red-fruit. Phytophthora capsici post inoculation, most of the CaChi (CaChiI3, CaChiIII1, CaChiIII2, CaChiIII4, CaChiIII6, CaChiIII7, CaChiIV1, CaChiVI1 and CaChiVI2) were induced by both strains (PC and HX-9). Under abiotic and exogenous hormonal treatments, the CaChiIII2, CaChiIII7, CaChiVI1 and CaChiVI2 were upregulated by abiotic stress, while CaChiI1, CaChiIII7, CaChiIV1 and CaChiIV2 responded to hormonal treatments. Furthermore, CaChiIV1-silenced plants display weakened defense by reducing (60%) root activity and increase susceptibility to NaCl stress. Gene ontology (GO) enrichment analysis revealed that CaChi genes primarily contribute in response to biotic, abiotic stresses and metabolic/catabolic process within the biological process category. These results exposed that CaChi genes are involved in defense response and signal transduction, suggesting their vital roles in growth regulation as well as response to stresses in pepper plant. In conclusion, these finding provide basic insights for functional validation of the CaChi genes in different biotic and abiotic stresses.

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Section: Discussionsupporting

confidence: 89%

Classification and Genome-Wide Analysis of Chitin-Binding Proteins Gene Family in Pepper (Capsicum annuum L.) and Transcriptional Regulation to Phytophthora capsici, Abiotic Stresses and Hormonal Applications

Ali

Luo

Khan

et al. 2018

IJMS

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“…GATA , bHLH ) and structural genes (e.g. ascorbate oxidase ( AAO ); dirigent , ( DIR )), which have previously been reported to have either direct or indirect effects on plant morphogenesis (Kato & Esaka, , ; Szécsi et al ., ; Wu et al ., ; Richter et al ., ), were also differentially expressed in GhWIP2OE and WT (Fig. c,d).…”

Section: Resultsmentioning

confidence: 99%

“…GhWIP2 may also acts upstream of the auxin signaling pathway to repress cell expansion by reducing the IAA concentration and down‐regulating several downstream signaling pathway genes, such as ARF and SAUR , which are involved in auxin‐associated plant cell expansion (Ren & Gray, ; Li et al ., ). Finally, we noted that GhWIP2 also regulates the expression of some structural genes, or TFs associated with cell expansion, such as AAO , DIR , GATA , and bHLH (Kato & Esaka, , ; Szécsi et al ., ; Wu et al ., ; Richter et al ., ), which are known to play roles in cell expansion (Fig. S15).…”

Section: Discussionmentioning

confidence: 99%

GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

Ren

Huang

et al. 2018

New Phytologist

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Cell expansion is a key determinant for the final size and shape of plant organ, and is regulated by various phytohormones. Zinc finger proteins (ZFPs) consist of a superfamily involved in multiple aspects of organ morphogenesis. However, little is known about WIP-type ZFP function in phytohormone-mediated organ growth. Using reverse genetics, RNA-seq and phytohormone quantification, we elucidated the role of a new WIP-type ZFP from Gerbera hybrida, GhWIP2, in controlling organ growth via regulation of cell expansion. GhWIP2 localizes to the nucleus and acts as a transcriptional repressor. Constitutive overexpression of GhWIP2 (GhWIP2OE) in both Gerbera and Arabidopsis thaliana caused major developmental defects associated with cell expansion, including dwarfism, short petals, scapes, and petioles. Furthermore, GhWIP2OE plants were hypersensitive to GA, but not to ABA, and showed a reduction in endogenous GA and auxin, but not ABA concentrations. Consistent with these observations, RNA-seq analysis revealed that genes involved in GA and auxin signaling were down-regulated, while those involved in ABA signaling were up-regulated in GhWIP2OE plants. Our findings suggest that GhWIP2 acts as a transcriptional repressor, suppressing cell expansion during organ growth by modulating crosstalk between GA, ABA, and auxin.

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“…However, the expression data suggest that OsPupK20 and OsPupK29 have root-specific functions. Dirigent proteins catalyze the polymerization of lignin monomers, a reaction that involves free radicals, and the dirigent gene family has been shown to play a role in plant defense response (Bhuiyan et al, 2009;Wu et al, 2009). Dirigent proteins also catalyze the synthesis of lignans, a diverse group of polyphenolic substances derived from Phe that may play a role in plant pathogen defense and are important anticancer substances in the human diet Lewis, 2000, 2005;Yoo et al, 2010).…”

Section: Pup1 Candidate Genesmentioning

confidence: 99%

Developing Rice with High Yield under Phosphorus Deficiency: Pup1 Sequence to Application

Chin

Gamuyao²,

Dalid³

et al. 2011

Plant Physiology

175

171

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The major quantitative trait locus (QTL) Phosphorus uptake1 (Pup1) confers tolerance of phosphorus deficiency in soil and is currently one of the most promising QTLs for the development of tolerant rice (Oryza sativa) varieties. To facilitate targeted introgression of Pup1 into intolerant varieties, the gene models predicted in the Pup1 region in the donor variety Kasalath were used to develop gene-based molecular markers that are evenly distributed over the fine-mapped 278-kb QTL region. To validate the gene models and optimize the markers, gene expression analyses and partial allelic sequencing were conducted. The markers were tested in more than 80 diverse rice accessions revealing three main groups with different Pup1 allele constitution. Accessions with tolerant (group I) and intolerant (group III) Pup1 alleles were distinguished from genotypes with Kasalath alleles at some of the analyzed loci (partial Pup1; group II). A germplasm survey additionally confirmed earlier data showing that Pup1 is largely absent from irrigated rice varieties but conserved in varieties and breeding lines adapted to drought-prone environments. A core set of Pup1 markers has been defined, and sequence polymorphisms suitable for singlenucleotide polymorphism marker development for high-throughput genotyping were identified. Following a marker-assisted backcrossing approach, Pup1 was introgressed into two irrigated rice varieties and three Indonesian upland varieties. First phenotypic evaluations of the introgression lines suggest that Pup1 is effective in different genetic backgrounds and environments and that it has the potential to significantly enhance grain yield under field conditions.

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Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica

Cited by 47 publications

References 16 publications

Classification and Genome-Wide Analysis of Chitin-Binding Proteins Gene Family in Pepper (Capsicum annuum L.) and Transcriptional Regulation to Phytophthora capsici, Abiotic Stresses and Hormonal Applications

Classification and Genome-Wide Analysis of Chitin-Binding Proteins Gene Family in Pepper (Capsicum annuum L.) and Transcriptional Regulation to Phytophthora capsici, Abiotic Stresses and Hormonal Applications

GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

Developing Rice with High Yield under Phosphorus Deficiency: Pup1 Sequence to Application

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