Cloning and expression analysis of MuNAC4 transcription factor protein from horsegram (Macrotyloma uniflorum (Lam.) Verdc.) conferred salt stress tolerance in Escherichia coli

Pandurangaiah, Merum; Reddy, K. Eswaranarayana; Rao, Gunupuru Lokanadha; Sivakumar, M.; Sudhakarbabu, O.; Nareshkumar, Ambekar; Ramya, Mummadireddy; Kirankumar, T.; Veeranagamallaiah, G.; Sudhakar, Chinta

doi:10.1007/s11738-012-1056-1

Cited by 12 publications

(3 citation statements)

References 27 publications

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“…The group 3 LEA protein PM2 from soybean imparted recombinant E. coli with improved tolerance to salt stress [53]. NAC4 protein from horse gram (Macrotyloma uniflorum) showed tolerance against salt (6 % NaCl), heavy metal (100 mM CuSO 4 ) and water stresses (6 % PEG) in E. coli cells [54]. The phenomenon of imparting stress tolerance in both prokaryotic and eukaryotic system may be due to a common protective mechanism acting in both prokaryotes and eukaryotes [40,55,56].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of drought stress responsive abscisic acid-stress-ripening (Asr 1) gene from wild jujube, Ziziphus nummularia (Burm.f.) Wight & Arn

et al. 2016

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Drought is a calamitous abiotic stress hampering agricultural productivity all over the world and its severity is likely to increase further. Abscisic acid-stress-ripening proteins (ASR), are a group of small hydrophilic proteins which are induced by abscisic acid, stress and ripening in many plants. In the present study, ZnAsr 1 gene was fully characterized for the first time from Ziziphus nummularia, which is one of the most low water forbearing plant. Full length ZnAsr 1 gene was characterised and in silico analysis of ZnASR1 protein was done for predicting its phylogeny and physiochemical properties. To validate transcriptional pattern of ZnAsr 1 in response to drought stress, expression profiling in polyethylene glycol (PEG) induced Z. nummularia seedlings was studied by RT-qPCR analysis and heterologous expression of the recombinant ZnAsr1 in Escherichia coli. The nucleotide sequence analysis revealed that the complete open reading frame of ZnAsr 1 is 819 bp long encoding a protein of 273 amino acid residues, consisting of a histidine rich N terminus with an abscisic acid/water deficit stress domain and a nuclear targeting signal at the C terminus. In expression studies, ZnAsr 1 gene was found to be highly upregulated under drought stress and recombinant clones of E. coli cells expressing ZnASR1 protein showed better survival in PEG containing media. ZnAsr1 was proven to enhance drought stress tolerance in the recombinant E.coli cells expressing ZnASR1. The cloned ZnAsr1 after proper validation in a plant system, can be used to develop drought tolerant transgenic crops.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of drought stress responsive abscisic acid-stress-ripening (Asr 1) gene from wild jujube, Ziziphus nummularia (Burm.f.) Wight & Arn

et al. 2016

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“…Yeasts have been shown to be an effective system that can be used to evaluate the tolerance of TF genes, such as EsDREB2B [ 49 ] and MuNAC [ 50 ]. The full ORF of the FtMYB22 was cloned and introduced into the pYES2 vector (Invitrogen, Carlsbad, CA, USA) with primers pYES2 - FtMYB22 -F and pYES2 - FtMYB22 -R. Then, the pYES2 - FtMYB22 and empty pYES2 (control) plasmids were transformed into yeast strain INVSc1 (Invitrogen, Carlsbad, CA, USA) using a lithium acetate procedure.…”

Section: Methodsmentioning

confidence: 99%

A Novel R2R3-MYB Transcription Factor FtMYB22 Negatively Regulates Salt and Drought Stress through ABA-Dependent Pathway

Zhao

Yao

Zhao

et al. 2022

IJMS

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Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse cereal with strongly abiotic resistance. The MYB family plays a regulatory role in plant growth, development, and responses to biotic and abiotic stresses. However, the characteristics and regulatory mechanisms of MYB transcription factors in Tartary buckwheat remain unclarified. Here, this study cloned the FtMYB22 gene from Tartary buckwheat, and investigated its involvement in responding to individual water deficit and salt stress in Arabidopsis. Sequence analysis highlighted that the N-termini of FtMYB22 contained two highly conserved SANT domains and one conserved domain from the SG20 subfamily. Nucleus-localized FtMYB22 did not have individual transcriptional activation activity. Water deficiency and salt stress induced the high expression of the GUS gene, which was driven by the promoter of FtMYB22. Yeast stress experiments showed that the overexpression of FtMYB22 significantly reduced the growth activity of transgenic yeast under water deficit or salt stress. Consistently, the overexpression of FtMYB22 reduced the salt and water deficit stress resistance of the transgenic plants. In addition, physiological parameters showed that transgenic plants had lower proline and antioxidant enzyme activity under stress conditions. Compared to the wild-type (WT), transgenic plants accumulated more malondialdehyde (MDA), H2O2, and O2−; they also showed higher ion permeability and water loss rates of detached leaves under stress treatments. Notably, FtMYB22 was involved in plant stress resistance through an ABA-dependent pathway. Under stress conditions, the expression of RD29A, RD29B, PP2CA, KIN1, COR15A, and other genes in response to plant stress in transgenic lines was significantly lower than that in the WT (p < 0.05). Furthermore, yeast two-hybrid assay showed that there was a significant interaction between FtMYB22 and the ABA receptor protein RCAR1/2, which functioned in the ABA signal pathway. Altogether, FtMYB22, as a negative regulator, inhibited a variety of physiological and biochemical reactions, affected gene expression and stomatal closure in transgenic plants through the ABA-dependent pathway, and reduced the tolerance of transgenic Arabidopsis to water deficiency and salt stress. Based on these fundamental verifications, further studies would shed light on the hormone signal response mechanism of FtMYB22.

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“…AhNAC2, when expressed in Arabidopsis, resulted in ABA hypersensitivity to attenuate root growth, seed germination, and stomatal closure, implying a role in positive ABA signaling (Liu et al 2011). Moreover, the expression of horse gram MuNAC4, improved long-term desiccation tolerance in peanut, accompanied by increased lateral root and improved osmotic adjustment and antioxidant activity (Pandurangaiah et al 2013). CarNAC3, an AtNAP like gene from chickpea (Cicer arietinum), conferred drought and salt tolerance in poplar, but reduced plant height (Movahedi et al 2015).…”

Section: Antagonist Regulation Of Growth and Stress Responsementioning

confidence: 99%

Balancing yield trade-off in legumes during multiple stress tolerance via strategic crosstalk by native NAC transcription factors

Sahoo

2021

J. Plant Biochem. Biotechnol.

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NAC transcription factors are the bonafide regulators of growth and stress-signaling. Disparate signals converge at NACmediated transcriptional programming to initiate a cellular response. Like other stress regulators such as DREB, DDF1, bZIP, PYL, NAC-mediated stress adaptation often compromises beneficial agronomic traits resulting in yield-penalty. Overexpression of these TFs can cause metabolic and hormonal perturbations that exert growth-retardation by activating ABA-hypersensitivity, chloroplast-degradation, or carbon-starvation, resulting in growth arrest and decline in photosynthetic activity. In addition, functional conservation of non-native members is unpredictable due to the non-conserved C-terminal part of the NAC proteins, hence limiting the utilization of well-studied orthologous genes in legumes. The growth/tolerance trade-off is an unresolved mystery. The co-occurrence of multiple stresses further perplexes the broadrange stress improvement of legume crops. To overcome the trade-offs, we need to understand the growth checkpoints that crosstalk with mediated by the stress-responsive gene candidate. Interestingly, NAC proteins appear to be the branch-point of ABA-dependent and ABA independent signaling hence can activate/repress a non-overlapping set of genes associated with stress response and growth to avoid the detrimental crosstalk. Indeed, a versatile NAC member improving both stress adaptation and yield simultaneously through synergistic crosstalk holds the key for sustainable legume improvement. Moreover, the successful manipulation of stress signaling depends on non-interfered ABA signaling of stress responses and growth, as well as strengthening of carbon assimilation. Photosynthesis plays a central role in plant carbohydrate metabolism and stress recovery by restoring the energy status. Hormones and carbohydrates participate together in a complex stress-signal transduction system. For instance, ABA antagonistically regulates growth and sugar signals. Native NAC candidates isolated from robust genetic sources like cowpea can be a useful approach to overcome trade-offs and achieve a desired phenotype. This review provides new insights to improve legume yield via transcriptional reprogramming.

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Cloning and expression analysis of MuNAC4 transcription factor protein from horsegram (Macrotyloma uniflorum (Lam.) Verdc.) conferred salt stress tolerance in Escherichia coli

Cited by 12 publications

References 27 publications

Molecular cloning and characterization of drought stress responsive abscisic acid-stress-ripening (Asr 1) gene from wild jujube, Ziziphus nummularia (Burm.f.) Wight & Arn

Molecular cloning and characterization of drought stress responsive abscisic acid-stress-ripening (Asr 1) gene from wild jujube, Ziziphus nummularia (Burm.f.) Wight & Arn

A Novel R2R3-MYB Transcription Factor FtMYB22 Negatively Regulates Salt and Drought Stress through ABA-Dependent Pathway

Balancing yield trade-off in legumes during multiple stress tolerance via strategic crosstalk by native NAC transcription factors

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