Cloning and Expression Analysis of Flavonoid 3′, 5′-Hydroxylase Gene from Brunfelsia acuminata

Abstract

The full-length cDNA sequence of F3′5′H gene from the Brunfelsia acuminata was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5′UTR and 61 bp 3′UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3′5′H was 91%, 87% and 84% with that of Petunia × hybrida, Nierembergia sp., Solanum tuberosum, respectively. Real-time quantitative PCR analysis showed that the expression of F3′5′H gene was different in petals of different days, which was the highest expression level on day 0 and significantly higher than other days. The results indicated that F3′5′H might play key role in flower color regulation and provide a theoretical reference for blue flower molecular breeding.

Integrating Transcriptomic and Metabolomic Analyses to Explore the Effect of Color Under Fruit Calyx on That of Fruit Apex in Eggplant (Solanum melongena L.)

“…In the current study, we conducted integrated transcriptome and metabolome analyses of samples from Z and L line fruits, transcriptome data of peel samples from three fruit regions were analyzed to identify the key DEGs and metabolic pathways, and then the content of metabolites of peel samples under the calyx and at the apex was analyzed by metabolome, the results of which facilitate understanding of the effect of the color under eggplant fruit calyx on that of the fruit apex. Chalcone isomerase (CHI), leucoanthocyanidin dioxygenase (LDOX), dihydroflavonol-4-reductase (DFR), and flavonoid 3′,5′-hydroxylase (F3′5′H) are key genes that determine flavonoid and anthocyanin metabolite content (Nan et al, 2021;Han et al, 2021;Li et al, 2021), which play important roles in color formation (Wang et al, 2018). CHI catalyzes the conversion of chalcones to flavanones (Chao et al, 2021;Ni et al, 2020) and the flavonoids generated include flavones, isoflavones, flavonols, and anthocyanidins (Nabavi et al, 2020).…”

“…For example, CaMYB306 accelerated fruit coloration in pepper ( Ma et al, 2022 ). While SmMYB86 and StMYB44 palyed negative regulators of the anthocyanin biosynthesis in eggplant peels and potato tubers, respectively ( Liu et al, 2019 ; Li et al, 2021 ). Additionally, bHLH TFs were also reported in regulation of anthocyanin biosynthesis ( Duan et al, 2021 ; Zhang et al, 2021 ).…”

“…Chalcone isomerase ( CHI ), leucoanthocyanidin dioxygenase ( LDOX ), dihydroflavonol-4-reductase ( DFR ), and flavonoid 3′,5′-hydroxylase ( F3′5′H ) are key genes that determine flavonoid and anthocyanin metabolite content (Nan et al, 2021; Han et al, 2021 ; Li et al, 2021 ), which play important roles in color formation ( Wang et al, 2018 ). CHI catalyzes the conversion of chalcones to flavanones ( Chao et al, 2021 ; Ni et al, 2020 ) and the flavonoids generated include flavones, isoflavones, flavonols, and anthocyanidins ( Nabavi et al, 2020 ).…”

“…Further, anthocyanin synthesis is promoted by increased LDOX gene expression ( Wu et al, 2020 ). DFR and F3′5′H have important roles in anthocyanin regulation ( Zhang et al, 2017 ; Wei et al, 2019 ; Li et al, 2021 ). In this study, compared with the peel under the calyx of L, levels of the CHI , LDOX , DFR, and F3′5′H genes were 185.46-, 252.34-, 149.59-, and 150.98-fold higher, respectively, in Z, and the trend in the fruit apex was consistent with that in samples from under the calyx at 10.91-, 10.42-, 6.23-, and 7.46-fold higher in Z.…”

Comparative RNA-Seq analysis to understand anthocyanin biosynthesis and regulations in Curcuma alismatifolia

“…Nowadays, a large number of cultivars, including 'Qiang Mai Pink', 'Qiang Mai Red', 'Dutch Red' and 'UB Snow 701', have been introduced in China. They are produced as commercial cut flowers or as potted plants in urban gardens (Li et al, 2021a). pigments exhibit a wide range of colours, from pink to blue-purple, and primarily consist of six common types of anthocyanidins, namely pelargonidin, cyanidin, delphinidin, peonidin, petunidin and malvidin.…”