Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene.

Blanco, Carlos; Ritzenthaler, Paul; Mata-Gilsinger, Mireille

doi:10.1128/jb.149.2.587-594.1982

Cited by 28 publications

(10 citation statements)

References 32 publications

(28 reference statements)

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“…The fumA fragment contained single targets for AvaI, EcoRI, FIG. 4. Restriction maps of the segments of bacterial DNA cloned in (a) the A fumA (6.2 kb) and (b) A fumB (8.2 kb) transducing phages.…”

Section: Resultsmentioning

confidence: 99%

“…Restriction targets for HindlIl (H), EcoRI (R), and PstI (P) and the fragment sizes (in kilobases) are indicated. Genetic symbols: terC, terminus of chromosome replication; g48, unidentified gene encoding 48-kilodalton product; fumA fumarase; manA, mannosephosphate isomerase; uidAoR, ,B-glucuronidase operon (4). The relative orientation of the physical and genetic maps is derived from Bouche (6).…”

Section: Resultsmentioning

confidence: 99%

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Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12

Guest¹,

Roberts²

1983

J Bacteriol

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Two classes offumarase-transducing phages, XfumA and XfumB, were isolated from populations of recombinant phages containing Hindlll fragments of Escherichia coli DNA; they were isolated by virtue of their ability to complement the metabolic lesion of a fumarase-negative mutant. The strongly complementing A fumA phages contained a 6.2-kilobase HindIlI fragment encoding: thefumA gene, located at 35.5 min in the E. coli linkage map and expressing the major fumarase activity; the mannosephosphate isomerase gene, manA; and an unidentified gene, g48. The three genes were located relative to the restriction map of the cloned fragment and the genetic linkage map (terC-g48-fumA-manA-uidAoR), their transcription polarities were defined as anticlockwise in the chromosome, and the molecular weights of the corresponding gene products were established: fumA, 61,500; manA, 42,000; g48, 48,000. Organisms containing the fumA gene subcloned in multicopy plasmids overproduced fumarase up to 50-fold. The weakly complementing class oftransducing phages, AfumB, contained several genes in an 8.2-kilobase Hindlll fragment, including one (fumB) that determines a minor fumarase activity. Complementation by fumB was only observed in high-copy situations such as transduction plaques and in strains containing a multicopy plasmid in which 40% of normal fumarase activity was detected. The basis for the complementation by fumB was not defined.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12

Guest¹,

Roberts²

1983

J Bacteriol

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“…The UidR regulon ( Table 2) allows for the growth of E. coli on the D-glucuronate and D-galacturonate moieties of the ␤-glucuronides and ␤-galacturonides present in mucus (10). After entering the cell, glucuronate and galacturonate are degraded via parallel pathways (Fig.…”

Section: Catabolism Of Hexuronates and Hexuronidesmentioning

confidence: 99%

What’s for Dinner?: Entner-Doudoroff Metabolism in Escherichia coli

1998

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“…When plasmids pREG8 and pREG9 were introduced into strain 2510 (lacZY deletion) and into strain HB101 (lacY mutation), a Lac' phenotype on lactose-containing MacConkey agar plates was restored only in the transformants of strain HB101 (6). This indicates that the BamHI fragment cloned into pMC874 possessed a promoter activity in the left and right directions When plasmid pRE2 carrying the exuR regulatory gene was added in trans in these transformants, 3-galactosidase synthesis was strongly reduced, suggesting that transcription of the two fused genes was regulated like transcription of the original exuT and uxaA genes, by the ExuR repressor ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

Physical mapping of the exuT and uxaC operators by use of exu plasmids and generation of deletion mutants in vitro

Mata-Gilsinger¹,

Ritzenthaler²

1983

J Bacteriol

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Bacterial strains, phages, and plasmids. The bacterial strains, phages, and plasmids used in this investigation are listed in Table 1. The bacterial strains were E. coli K-12 derivatives.Culture media. The media used for growth were identical to those described by Miller (26). The synthetic medium used was M63 medium (pH 7.2) (44) or M9 medium (pH 7.2) (26) and contained either glucose (5 g/liter), glycerol (5 g/liter), glucuronate (2.5 g/liter), galacturonate (2.5 g/liter), tagaturonate (2.5 g/liter), or fructuronate (2.5 g/liter); aldohexuronate-containing MacConkey media (Difco Laboratories) contained 15 mg of glucuronate or galacturonate per ml. When needed, ampicillin, chloramphenicol, and kanamycin were used at final concentrations of 25 jig/ml, and 973 on August 5, 2020 by guest

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Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene.

Cited by 28 publications

References 32 publications

Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12

Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12

What’s for Dinner?: Entner-Doudoroff Metabolism in Escherichia coli

Physical mapping of the exuT and uxaC operators by use of exu plasmids and generation of deletion mutants in vitro

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