Cloning and differential expression of manganese superoxide dismutase (Mn-SOD) of Trichinella pseudospiralis

Wu, Weicheng; Mak, C. H.; Ko, Ronald C.

doi:10.1007/s00436-007-0755-9

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…The manganese SOD of T. pseudospiralis was reported by Wu et al (2007a). This manganese SOD is a mitochondrial enzyme because of the presence of mitochondrial transit peptides and maturation cleavage site in this protein.…”

Section: Superoxide Dismutasesmentioning

confidence: 99%

Functional genes and proteins of Trichinella spp.

Nagano

Takahashi

2008

Parasitol Res

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Research of Trichinella proteins has been conducted with emphasis on excretory-secretory (E-S) products of muscle larvae because of two reasons. The first is that it has prominent and narrow specific antigenicity, and the second is that it may play some role in nurse cell formation after being secreted into host muscle cells. Proteomic analysis of E-S proteins was further advanced by the aid of new analytical methods such as gene cloning, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, and expressed sequence tags database analysis. As the research progressed, the interest of researchers moved to identification of function of E-S products, which has shed further light on the intriguing relationships between parasites and hosts. Major constituents of the E-S products include 43-, 53-, and 45-kDa glycoprotein derived from the stichosome. Many proteins were discovered in E-S products after the 43-, 53-, and 45-kDa proteins although the relationships among them remain unclear. Some of the new proteins were partially defined in terms of their function including nuclear antigens, MyoD-like protein, TsJ5 protein, etc. There are better-characterized proteins based on the gene molecular method, which allow easier identification of the function of proteins of interest. Such examples were demonstrated by proteinases, proteinase inhibitors, heat shock proteins, glycosidases, etc.

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Section: Superoxide Dismutasesmentioning

confidence: 99%

Functional genes and proteins of Trichinella spp.

Nagano

Takahashi

2008

Parasitol Res

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“…Our previous study of the Cu/Zn-SOD of S. erinacei suggested that its primary role in parasite survival was in protecting the parasite from endogenous oxidative stress as well as detoxifying exogenous superoxide anions (Li et al, 2010). Compared with the extensive work that has been performed on the Cu/Zn-SODs of helminth parasites, only a few helminth Mn-SODs have been identified (Henkle-Dü hrsen et al, 1995;Li, Kong et al, 2005;Wu et al, 2008), and information on their biochemical and biological properties is limited. Considering the mitochondrial erinacei.…”

Section: Discussionmentioning

confidence: 99%

“…These studies collectively suggest the critical roles that mitochondrial SODs play in the maintenance of cellular physiology. It is well known that the extracellular and cytosolic SODs (mostly Cu/Zn-SODs) of helminth parasites play essential roles in parasite survival in the host by detoxifying superoxide anions produced endogenously by the parasite as well as the host immune effector cells (Henkle et al, 1991;Hong et al, 1992;Tang et al, 1994;Kim et al, 2000;Piedrafita et al, 2000;Tawe et al, 2000;Castellanos-Gonzá lez et al, 2002;Li, Na et al, 2005;Li, Kong et al, 2005;Lee et al, 2005;Wu et al, 2006). Our previous study of the Cu/Zn-SOD of S. erinacei suggested that its primary role in parasite survival was in protecting the parasite from endogenous oxidative stress as well as detoxifying exogenous superoxide anions (Li et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…They play an essential role in protecting organisms from oxidative damages from superoxide anions, which are produced by both enzymatic and non-enzymatic metabolic processes (Fridovish, 1998). SODs have been identified and characterized in various helminth parasites, and their critical roles in parasite survival in hosts have been well addressed (Henkle et al, 1991;Hong et al, 1992;Tang et al, 1994;Henkle-Dü hrsen et al, 1995;Kim et al, 2000;Tawe et al, 2000;Castellanos-Gonzá lez et al, 2002;Lee et al, 2005;Li, Kong et al, 2005;Li, Na et al, 2005;Wu et al, 2006Wu et al, , 2008 Li et al, 2010). In a previous study, we characterized a copper-zinc SOD (Cu/Zn-SOD) of S. erinacei (Li et al, 2010).…”

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confidence: 99%

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Identification and Characterization of A Mitochondrial Manganese Superoxide Dismutase of Spirometra erinacei

Song

et al. 2011

Journal of Parasitology

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A gene encoding the manganese superoxide dismutase (Mn-SOD) of Spirometra erinacei was identified, and the biochemical properties of the recombinant enzyme were partially characterized. The S. erinacei Mn-SOD gene consisted of 669 bp, which encoded 222 amino acids. A sequence analysis of the gene showed that it had typical molecular structures, including characteristic metal-binding residues and motifs that were conserved in Mn-SODs. An analysis of the N-terminal presequence of S. erinacei Mn-SOD revealed that it had physiochemical characteristics commonly found in mitochondria-targeting sequences and predicted that the enzyme is located in the mitochondria. A biochemical analysis also revealed that the enzyme is a typical Mn-SOD. The enzyme was consistently expressed in both S. erinacei plerocercoid larvae and adult worms. Our results collectively suggested that S. erinacei Mn-SOD is a typical mitochondrial Mn-SOD and may play an important role in parasite physiology, detoxifying excess superoxide radicals generated in the mitochondria.Spirometra erinacei is a pseudophyllidean tapeworm that inhabits the intestines of cats and dogs. The infections are usually asymptomatic, but in more severe cases, the infected animals may exhibit weight loss, irritability, and changes in appetite. The most important clinical manifestation, sparganosis, is developed when the plerocercoid larvae of the parasite (spargana) infect other intermediate vertebrate hosts, including humans. Human infections are mainly acquired by ingesting water containing Cyclops spp. in which tapeworm eggs mature into procercoid larvae, by ingesting uncooked mammalian, reptilian, or amphibian flesh, or by placing frog poultices on open lesions (Sarma and Weilbaecher, 1986). Spargana can invade muscle, subcutaneous tissues, visceral and urogenital organs, orbital tissue, and occasionally the central nervous system, causing inflammation and fibrosis (Chang et al., 1992). Symptoms differ depending on the particular tissues or organs involved.Highly reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide, hydroxyl radicals, and singlet oxygen, are generated through numerous pathways in aerobic organisms (Fridovich, 1995). ROS induce peroxidation of membrane lipids, DNA damage, and inactivation of proteins (Imlay and Linn, 1988). Oxidative stress resulting from ROS is much greater in parasitic organisms because they must detoxify ROS produced endogenously as well as those produced by host immune effector cells. Activated host immune effector cells undergo an oxidative burst and produce ROS as a part of their defense against parasitic infections (Butterworth, 1984;Callahan et al., 1988;Brophy and Pritchard, 1992;Maizels et al., 1993). Therefore, effective detoxification of host-generated ROS is important for parasitic organisms in evading host immune responses and for surviving inside the host (Callahan et al., 1988;Mkoji et al., 1988;Nare et al., 1990;Batra et al., 1993;Maizels et al., 1993;Vaca-Paniagua et al., 2008;Mourã o ...

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“…They play essential roles in protecting organisms from oxidation by the superoxide anion which is produced by enzymatic and non-enzymatic metabolic pathways (Fridovich 1998). SODs have been identified and characterized in various helminth parasites, and their essential roles for parasite survival in the hosts have been well addressed (Henkle et al 1991;Hong et al 1992;HenkleDührsen et al 1995;Kim et al 2000;Tawe et al 2000;Castellanos-González et al 2002;Lee et al 2005;Li et al 2005a, b;Wu et al 2008). Several studies have also suggested the potential of SODs of parasitic helminths as vaccine candidates (Shalaby et al 2003;Carvalho-Queiroz et al 2004;Cook et al 2004;Vaca-Paniagua et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei

Ahn

et al. 2010

Parasitol Res

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Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a gene encoding the copper/zinc-superoxide dismutase of S. erinacei (SeCuZn-SOD) and partially characterized the biochemical and functional properties of the enzyme. The open reading frame of SeCuZnSOD was 465 bp that encodes 154 amino acids. The characteristic amino acid residues and motifs required for coordinating copper and zinc enzymatic function were well conserved. The genomic length of the SeCuZnSOD was 1,985 bp consisting of three exons that are separated by two introns. SeCuZnSOD is a typical cytosolic form which shares similar biochemical properties, including broad pH optima and inhibition profile by KCN and H 2 O 2 , with cytosolic Cu/Zn-SODs of other organisms. SeCuZnSOD was functionally expressed in both S. erinacei plerocercoid larvae and adult worms, and its expression level was significantly increased when the plerocercoid larvae were treated with paraquat. The enzyme may play essential roles for survival of the parasite not only by protecting itself from endogenous oxidative stress, but also by detoxifying oxidative killing of the parasite by host immune effector cells.

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Cloning and differential expression of manganese superoxide dismutase (Mn-SOD) of Trichinella pseudospiralis

Cited by 9 publications

References 29 publications

Functional genes and proteins of Trichinella spp.

Functional genes and proteins of Trichinella spp.

Identification and Characterization of A Mitochondrial Manganese Superoxide Dismutase of Spirometra erinacei

Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei

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