Cloning and developmental expression of Shaker potassium channels in the cochlea of the chicken

Duzhyy, Dmytro E.; Sakai, Yoshihisa; Sokolowski, Bernd

doi:10.1016/j.molbrainres.2003.10.022

Cited by 8 publications

(17 citation statements)

References 32 publications

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“…For example, Kv1.2 and Kv1.5 have been shown to form heteromultimeric complexes in vascular smooth muscle cells [29, 30], while Kv1.3 and Kv1.5 association has been reported in macrophages [31]. We utilized previously published primer sets [23] to determine the presence of Kv1.2, Kv1.3, Kv1.5, and Kv1.8 channels in the genomic DNA of E12 chickens (Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

“…Equal amounts of RNA were reversed transcribed and equal amounts of cDNA were amplified using the One Taq RT-PCR kit from New England Biolabs. Previously published primer sets were used to amplify gene fragments specific to Kv1 channels and -actin primers were used as a positive control for a reference gene [23]. Because Kv1 family channel genes are intronless, all cDNA synthesis reactions were run side by side with a no reverse transcriptase (RT) condition as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…Homomultimeric or heteromultimeric assembly of these subunits through a T1 tetramerization domain leads to the formation of functional Kv1 ion channels [4, 21, 22]. Four specific intronless alpha subunits, Kv1.2, Kv1.3, Kv1.5 and Kv1.8, have been cloned in the chicken genome and demonstrate conservation of important mammalian structural regions including the T1 tetramerization domain, the ion transport domain, and the PDZ-binding domain [23]. However, whether Kv1 channel regulation plays a role in the electrophysiological development of embryonic hypothalamic circuits remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Several reports indicate that Kv1 family channels are present in avian tissues [23, 24, 25, 26, 27]. For example, Kv1.2, Kv1.3 and Kv1.5 mRNA has been detected in several regions of the embryonic avian cochlea [23].…”

Section: Introductionmentioning

confidence: 99%

“…For example, Kv1.2, Kv1.3 and Kv1.5 mRNA has been detected in several regions of the embryonic avian cochlea [23]. Additional studies demonstrate Kv1.1 and Kv1.2 immunoreactivity in neuronal cell bodies of the chick tangential nucleus [27].…”

Section: Introductionmentioning

confidence: 99%

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Developmental expression of Kv1 voltage-gated potassium channels in the avian hypothalamus

Doczi

Vitzthum

Forehand

2016

Neuroscience Letters

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Specialized hypothalamic neurons integrate the homeostatic balance between food intake and energy expenditure, processes that may become dysregulated during the development of diabetes, obesity, and other metabolic disorders. Shaker family voltage-gated potassium channels (Kv1) contribute to the maintenance of resting membrane potential, action potential characteristics, and neurotransmitter release in many populations of neurons, although hypothalamic Kv1 channel expression has been largely unexplored. Whole-cell patch clamp recordings from avian hypothalamic brain slices demonstrate a developmental shift in the electrophysiological properties of avian arcuate nucleus neurons, identifying an increase in outward ionic current that corresponds with action potential maturation. Additionally, RT-PCR experiments identified the early expression of Kv1.2, Kv1.3, and Kv1.5 mRNA in the embryonic avian hypothalamus, suggesting that these channels may underlie the electrophysiological changes observed in these neurons. Real-time quantitative PCR analysis on intact microdissections of embryonic hypothalamic tissue revealed a concomitant increase in Kv1.2 and Kv1.5 gene expression at key electrophysiological time points during development. This study is the first to demonstrate hypothalamic mRNA expression of Kv1 channels in developing avian embryos and may suggest a role for voltage-gated ion channel regulation in the physiological patterning of embryonic hypothalamic circuits governing energy homeostasis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Developmental expression of Kv1 voltage-gated potassium channels in the avian hypothalamus

Doczi

Vitzthum

Forehand

2016

Neuroscience Letters

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Protein–protein interactions of a Kvβ subunit in the cochlea

Sokolowski

Harvey

Venkataramu

et al. 2005

J of Neuroscience Research

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Accessory subunits associated with voltage-gated potassium (Kv) channels can influence the biophysical properties and promote the surface expression of channel-forming alpha-subunits. Previously, we cloned several alpha-subunits and a beta-subunit from a cDNA library of the chicken cochlea. In the present study, we raised an antibody against the N-terminus of chicken Kvbeta1.1 (cKvbeta1.1) and characterized the Kvbeta-related polypeptide in cochlear tissues and heterologous cells. The anti-cKvbeta1.1 antibody recognizes a 45-kDa polypeptide in chick cochlear extracts as well as in Chinese hamster ovary (CHO) cells transfected with cKvbeta1.1. The accessory subunit was localized to the ganglion cells of the chick cochlea using immunohistochemistry and in situ hybridization. Coimmunoprecipitation studies show that Kvbeta1.1 interacts with Shaker channel members Kvalpha1.2 and 1.3, both of which colocalize with beta to the cochlear ganglion cells. Additionally, coimmunoprecipitation studies show that Kvalpha1.2 and 1.3 interact with each other, suggesting that these ion channels are formed by heteromultimers. In comparison, Kvbeta did not coprecipitate with a member of the Shal subfamily. The presence of Kvbeta in the cochlea suggests that this subunit contributes to the modulation of auditory signals in the ganglion cells, presumably by regulating properties of inactivation as well as surface expression of Kvalpha channels.

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Voltage-gated potassium channel Kv1.3 in rabbit ciliary epithelium regulates the membrane potential via coupling intracellular calcium

Zhuo²,

Bi³

et al. 2008

Chinese Medical Journal

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Cloning and developmental expression of Shaker potassium channels in the cochlea of the chicken

Cited by 8 publications

References 32 publications

Developmental expression of Kv1 voltage-gated potassium channels in the avian hypothalamus

Developmental expression of Kv1 voltage-gated potassium channels in the avian hypothalamus

Protein–protein interactions of a Kvβ subunit in the cochlea

Voltage-gated potassium channel Kv1.3 in rabbit ciliary epithelium regulates the membrane potential via coupling intracellular calcium

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