Cloning and Comparison of
            <i>fliC</i>
            Genes and Identification of Glycosylation in the Flagellin of
            <i>Pseudomonas aeruginosa</i>
            a-Type Strains

Brimer, Cynthia; Montie, Thomas C.

doi:10.1128/jb.180.12.3209-3217.1998

Cited by 110 publications

(35 citation statements)

References 33 publications

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“…Sixty‐seven of 123 (55%) keratitis isolates did not show hybridisation for either marker pKL‐1 or pKL3 compared to 122 of 322 (38%) nonkeratitis isolates ( P = 0.05). P. aeruginosa a‐type flagellins vary because of the presence of a glycosylation island (Brimer & Montie, ; Arora et al ., ) that can be present as either a longer insert encompassing 14 ORFs, or as a shorter version with a 5.4‐kb deletion (Arora et al ., ). Twenty of 123 (16%) keratitis isolates carried the full length glycosylation island (12 of 63 isolates in 2003–2004 and 8 of 60 isolates in 2009–2010) and 61 of 123 (50%) carried the truncated version.…”

Section: Resultsmentioning

confidence: 99%

Genotypic analysis of UK keratitis-associated Pseudomonas aeruginosa suggests adaptation to environmental water as a key component in the development of eye infections

Shankar

Sueke

Wiehlmann

et al. 2012

FEMS Microbiol Lett

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To examine temporal dynamics of corneal infection (keratitis)‐associated Pseudomonas aeruginosa, we compared the genetic characteristics of isolates collected during two different time periods (2003–2004 and 2009–2010) using an ArrayTube genotyping system. The distribution of keratitis‐associated isolates from the two studies (n = 123) among a database of P. aeruginosa strains of non‐ocular origin (n = 322) indicated that 71% of UK keratitis‐associated P. aeruginosa isolates clustered together, and there was no evidence for major variations in the distribution of clone types between the two collections. Our analysis indicates the presence of a ‘core keratitis cluster’, associated with corneal infections, that is related to the P. aeruginosa eccB clonal complex, which is associated with adaptation to survival in environmental water. This suggests that adaptation to environmental water is a key factor in the ability of P. aeruginosa to cause eye infections.

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Section: Resultsmentioning

confidence: 99%

Genotypic analysis of UK keratitis-associated Pseudomonas aeruginosa suggests adaptation to environmental water as a key component in the development of eye infections

Shankar

Sueke

Wiehlmann

et al. 2012

FEMS Microbiol Lett

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“…Brimer and Montie [33] demonstrated that type A, but not type B, £agellins are glycosylated but the structure of the glycosylation has not yet been de¢ned. Flagella of P. aeruginosa primarily consist of a FliC (£agellin) subunit.…”

Section: Flagellin Glycosylation Genes (Pak)mentioning

confidence: 99%

The genetics of glycosylation in Gram-negative bacteria

Power

Jennings

2003

FEMS Microbiology Letters

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In recent years there has been a dramatic increase in reports of glycosylation of proteins in various Gram-negative systems including Neisseria meningitidis, Neisseria gonorrhoeae, Campylobacter jejuni, Pseudomonas aeruginosa, Escherichia coli, Caulobacter crescentus, Aeromonas caviae and Helicobacter pylori. Although this growing list contains many important pathogens (reviewed by Benz and Schmidt [Mol. Microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pathogenesis such as pili, adhesins and flagella the precise role(s) of the glycosylation of these proteins remains to be determined. Furthermore, the details of the glycosylation biosynthetic process have not been determined in any of these systems. The definition of the precise role of glycosylation and the mechanism of biosynthesis will be facilitated by a detailed understanding of the genes involved.

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“…Genomic diversity resulting from horizontal gene transfer suggests a possibility of acquisition of novel virulence traits in P. aeruginosa. For example, flagella of some P. aeruginosa strains are often glycosylated (85). Very recently, it has been reported (77) that another genomic island of ca.…”

Section: Genetic Diversity and Genomic Islandsmentioning

confidence: 99%

Persistent infections and immunity in cystic fibrosis

Yu¹

2002

Front Biosci

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Cystic fibrosis (CF) is the most common autosomal recessive lethal disease in the Caucasian population. Chronic respiratory infections with Pseudomonas aeruginosa, neutrophil-dominated airway inflammation and progressive lung damage are the major causes of morbidity and mortality in CF. Two persistent infection phenotypes expressed by this bacterium are biofilm and mucoidy. Biofilm, also called the microcolony mode of growth is the surface-associated adherent bacterial community, while mucoidy refers to a phenotype conducive to copious amounts of mucoid exopolysaccharide (MEP)/alginate that provides a matrix for mature biofilms conferring resistance to host defenses and antibiotics. Recent completion of the whole genomic sequence of the standard reference strain P. aeruginosa PAO1 has led to discoveries that many clinical isolates of this species possess unique genomic sequences (genomic islands) due to horizontal gene transfer. We propose this type of genetic exchange may play an important role in causing intrinsic genomic diversity of this organism. Therefore, the diversity, as revealed through profiles of restriction fragment length polymorphism (RFLP), may be linked to an array of novel and unexplored pathogenic mechanisms in P. aeruginosa. CF mouse models, while displaying many clinical similarities to human CF, have yet to demonstrate a chronic pulmonary disease phenotype. This review is intended to provide an overview of P. aeruginosa persistent infection phenotypes (biofilm and mucoidy) and an aerosol infection mouse model for CF. Genomic diversity of P. aeruginosa and its implications in the pathogenesis in CF will also be discussed.

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Cloning and Comparison of fliC Genes and Identification of Glycosylation in the Flagellin of Pseudomonas aeruginosa a-Type Strains

Cited by 110 publications

References 33 publications

Genotypic analysis of UK keratitis-associated Pseudomonas aeruginosa suggests adaptation to environmental water as a key component in the development of eye infections

Genotypic analysis of UK keratitis-associated Pseudomonas aeruginosa suggests adaptation to environmental water as a key component in the development of eye infections

The genetics of glycosylation in Gram-negative bacteria

Persistent infections and immunity in cystic fibrosis

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