Cloning and characterization of two lipopolysaccharide-binding protein/bactericidal permeability–increasing protein (LBP/BPI) genes from the sea cucumber Apostichopus japonicus with diversified function in modulating ROS production

Shao, Yina; Li, Chenghua; Che, Zhongjie; Zhang, Pengjuan; Zhang, Weiwei; Duan, Xuemei; Li, Ye

doi:10.1016/j.dci.2015.04.015

Cited by 38 publications

(15 citation statements)

References 48 publications

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“…Our differential expression results also identified AMP transcripts including a bactericidal permeability-increasing protein (BPIP) and achacin. BPIP kills gram negative bacteria by targeting the lipopolysaccharide outer cell membrane [63][64][65][66][67]. Achacin, an AMP present in African Giant Slug mucus, has potent gram-positive and gram-negative bacteria killing properties [68][69][70].…”

Section: Corals With Visual Disease Transmission Activate An Innate Imentioning

confidence: 99%

Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events

et al. 2020

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Coral disease outbreaks are expected to increase in prevalence, frequency and severity due to climate change and other anthropogenic stressors. This is especially worrying for the Caribbean branching coral Acropora palmata which has already seen an 80% decrease in cover primarily due to disease. Despite the importance of this keystone species, there has yet to be a characterization of its transcriptomic response to disease exposure. In this study we provide the first transcriptomic analysis of 12 A. palmata genotypes and their symbiont Symbiodiniaceae exposed to disease in 2016 and 2017. Year was the primary driver of gene expression variance for A. palmata and the Symbiodiniaceae. We hypothesize that lower expression of ribosomal genes in the coral, and higher expression of transmembrane ion transport genes in the Symbiodiniaceae indicate that a compensation or dysbiosis may be occurring between host and symbiont. Disease response was the second driver of gene expression variance for A. palmata and included a core set of 422 genes that were significantly differentially expressed. Of these, 2 genes (a predicted cyclin-dependent kinase 11b and aspartate 1-decarboxylase) showed negative Log2 fold changes in corals showing transmission of disease, and positive Log2 fold changes in corals showing no transmission of disease, indicating that these may be important in disease resistance. Co-expression analysis identified two modules positively correlated to disease exposure, one enriched for lipid biosynthesis genes, and the other enriched in innate immune genes. The hub gene in the immune module was identified as D-amino acid oxidase, a gene implicated in phagocytosis and microbiome homeostasis. The role of D-amino acid oxidase in coral immunity has not been characterized but could be an important enzyme for responding to disease. Our results indicate that A. palmata mounts a core immune response to disease exposure despite differences in the disease type and virulence between 2016 and 2017. These identified genes may be important for future biomarker development in this Caribbean keystone species.

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Section: Corals With Visual Disease Transmission Activate An Innate Imentioning

confidence: 99%

Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events

et al. 2020

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show abstract

“…Our differential expression results also identified transcripts annotating to AMPs; a bactericidal permeability-increasing protein which has gram-negative bacteria killing properties by targeting the lipopolysaccharide outer layer [72–76], and Achacin, an AMP present in African Giant Slug mucus that has potent gram-positive and gram-negative bacteria killing properties [77–79]. To our knowledge, these AMPs have not been characterized in any other coral disease studies.…”

Section: Discussionmentioning

confidence: 91%

Innate immune gene expression inAcropora palmatais consistent despite variance in yearly disease events

Young

Serrano

Rosales

et al. 2020

Preprint

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Coral disease outbreaks are expected to increase in prevalence, frequency and severity due to climate change and other anthropogenic stressors. This is especially worrying for the Caribbean branching Acropora palmata which has already seen an 80% decrease in its coral cover, with this primarily due to disease. Despite the importance of this species, there has yet to be a characterization of its transcriptomic response to disease exposure. In this study we provide the first transcriptomic analysis of 12 A. palmata genotypes, and their symbiont Symbiodiniaceae, exposed to disease in 2016 and 2017. Year was the primary driver of sample variance for A. palmata and the Symbiodiniaceae. Lower expression of ribosomal genes in the coral, and higher expression of transmembrane ion transport genes in the Symbiodiniaceae indicate that the increased virulence in 2017 may have been due to a dysbiosis between the coral and Symbiodiniaceae. We also identified a conserved suite of innate immune genes responding to the disease challenge that was activated in both years. This included genes from the Toll-like receptor and lectin pathways, and antimicrobial peptides. Co-expression analysis identified a module positively correlated to disease exposure rich in innate immune genes, with D-amino acid oxidase, a gene implicated in phagocytosis and microbiome homeostasis, as the hub gene. The role of D-amino acid oxidase in coral immunity has not been characterized but holds potential as an important enzyme for responding to disease. Our results indicate that A. palmata mounts a similar immune response to disease exposure as other coral species previously studied, but with unique features that may be critical to the survival of this keystone Caribbean species.

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“…BPIFB1 and BPIFB2 belong to the bactericidal/permeabilityincreasing-fold-containing family 21 . BPIFB1 and BPIFB2 protein are most highly expressed in the trachea and lung 22 and bind to the Gram-negative bacteria and exert antibacterial function 23,24 . Moreover, it has been reported that BPIFB1 is abnormally expressed in tumors, which suggests that it plays a role in tumor development 25 .…”

Section: Discussionmentioning

confidence: 99%

Clinical and Prognostic Implications of an Immune-Related Risk Model Based on TP53 Status in Lung Adenocarcinoma

Song

Chen

Wang

et al. 2020

Preprint

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Background: TP53 mutation is the most widespread mutation in lung adenocarcinoma (LUAD), meanwhile p53 (encoded by TP53) has recently been implicated in immune responses. However, it is still unknown whether TP53 mutation may remodel tumor microenvironment to influence tumor progression and prognosis in LUAD.Methods: we developed a six-gene immune-related model (IRM) to predict the survival of patients with LUAD in TCGA cohort based on TP53 status using LASSO Cox analysis, which was also confirmed the predictive ability in two independent cohorts.Results: The mutation of TP53 led to a decrease in the immune response in LUAD. Further analysis revealed that patients in the high-index group had observably lower relative infiltration of memory B cells and regulatory T cells, together with significantly higher relative infiltration proportions neutrophils and resting memory CD4+ T cells. Additionally, the IRM index positively correlated with expression of critical immune checkpoint genes including PDCD1 (encoding PD-1) and CD274 (encoding PD-L1), which was validated in Nanjing cohort. Furthermore, the IRM index as an independent prognostic factor was used to establish a nomogram for clinical application.Conclusion: This immune-related model may serve as a powerful prognostic tool to further optimize immunotherapies for LUAD.

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Cloning and characterization of two lipopolysaccharide-binding protein/bactericidal permeability–increasing protein (LBP/BPI) genes from the sea cucumber Apostichopus japonicus with diversified function in modulating ROS production

Cited by 38 publications

References 48 publications

Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events

Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events

Innate immune gene expression inAcropora palmatais consistent despite variance in yearly disease events

Clinical and Prognostic Implications of an Immune-Related Risk Model Based on TP53 Status in Lung Adenocarcinoma

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