Cloning and Characterization of Two Novel Genes, cry24B and s1orf2, from a Mosquitocidal Strain of Bacillus thuringiensis serovar sotto

Ohgushi, Akira; Saitoh, Hiroyuki; Wasano, Naoya; Uemori, Akiko; Ohba, Michio

doi:10.1007/s00284-005-7529-3

Cited by 14 publications

(8 citation statements)

References 19 publications

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“…According to the pBtoxis plasmid sequence, these sequences exist, but lack their N-terminal half. Several reports support this observation as well as natural disconnections of the N-terminal half from its C-terminal half (Ito et al 2002;Ohgushi et al 2005;Hernández-Soto et al 2009;Noguera and Ibarra 2010;Barboza-Corona et al 2012;Sun et al 2013). In any case, this validation indicated that the developed HMM profile was able to detect any C-terminal sequence of a Cry protein, independently of the presence of its N-terminal half.…”

Section: Discussionsupporting

confidence: 55%

Search for Cry proteins expressed by Bacillus spp. genomes, using hidden Markov model profiles

2019

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This report focuses on a systematic search for Cry proteins in Bacillus spp. other than B. thuringiensis by analyzing reported Bacillus spp. genomes, using conserved sequences from the C-terminal half of reported Cry proteins in hidden Markov model profiles. A high-throughput model based on the use of HMMER and CD-HIT tools was designed, which identified Cry proteins. This model was used on 857 reported Bacillus spp. genomes, where 174 Cry protein sequences were identified, mostly, as expected, in B. thuringiensis genomes but, interestingly, 42 were identified on other species. Despite including 89 species of Bacillus in the HMMER analysis, Cry protein sequences were found only in genomes from species within the B. cereus group. According to the species registered at the NCBI database containing each genome, this group was formed by 18 non-B. thuringiensis strains. However, when sequences in those genomes were analyzed by multilocus sequence typing, the number of non-B. thuringiensis strains increased to 39, indicating that as many as 119 Cry protein sequences were found in four non-B. thuringiensis species. Therefore, dispersion of Cry proteins is much wider and frequent than previously thought, questioning its role in nature.

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Section: Discussionsupporting

confidence: 55%

Search for Cry proteins expressed by Bacillus spp. genomes, using hidden Markov model profiles

2019

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“…Figure 1 shows the configuration of various mosquitocidal Bt toxin genes including cry10A [16], cry19A [17,18], cry24B [19], cry30C [20], cry39A [21], cry40A (GenBank accession No. AB074414), cry44A [22], and cry59A [23].…”

Section: Separate Crystallization Domain Orfmentioning

confidence: 99%

In Vivo Crystallization of Three-Domain Cry Toxins

Adalat

Saleem

Crickmore

et al. 2017

Toxins

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Bacillus thuringiensis (Bt) is the most successful, environmentally-friendly, and intensively studied microbial insecticide. The major characteristic of Bt is the production of proteinaceous crystals containing toxins with specific activity against many pests including dipteran, lepidopteran, and coleopteran insects, as well as nematodes, protozoa, flukes, and mites. These crystals allow large quantities of the protein toxins to remain stable in the environment until ingested by a susceptible host. It has been previously established that 135 kDa Cry proteins have a crystallization domain at their C-terminal end. In the absence of this domain, Cry proteins often need helper proteins or other factors for crystallization. In this review, we classify the Cry proteins based on their requirements for crystallization.

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“…In vivo recombination, therefore, appears to be a promising approach to the creation of new B. sphaericus strains for vector control [86]. The results suggested that the Cry27A protein is responsible for the Anopheles-preferential toxicity of the B. thuringiensis serovar high strain [87]. These inclusions exhibited no larvicidal activities against three mosquito species: Aedes aegypti, Anopheles stephensi and Culex pipiens molestus.…”

Section: Transgenic Mosquitoesmentioning

confidence: 99%

Current Trends in the Control of Mosquito Vectors by Means of Biological Larvicides

Poopathi¹

2012

J Biofertil Biopestici

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Bacillus sphaericus Neide (B) and B. thuringiensis serovar israelensis (Bti) de Barjac provides effective alternatives to broad spectrum larvicides in many situations, with little or no environmental impact. Taking into account environmental benefits including safety for humans and other non-target organisms, reduction of pesticide residues in the aquatic environment, increased activity of most other natural enemies and increased biodiversity in aquatic ecosystems, their advantages are numerous. In addition to recombinant bacteria used as larvicides, research is also underway to develop transgenic algae and cyanobacteria using larvicidal endotoxins of Bti and Bs. The advent of recombinant DNA technology is now having an enormous impact on agriculture and medicine, and it is appropriate that the ability to manipulate and recombine genes with this technology be applied to improving larvicides for vector control. These new recombinant bacteria are as potent as many synthetic chemical insecticides, yet are much less prone to resistance, as they typically contain a mixture of endotoxins with different modes of action. The existing recombinants also have what can be considered disadvantageous, in that they do not show significantly improved activity against aedine and anopheline mosquitoes, in comparison to Bti. But it may be possible to overcome this limitation using some of the newly discovered mosquitocidal proteins, such as the Mtx proteins and peptides such as the trypsin-modulating oostatic factor, which could be easily engineered for high expression in recombinant bacteria. While other microbial technologies such as recombinant algae and other bacteria are being evaluated, it has yet to be shown that these are as efficacious and environment friendly as Bti and Bs. By combining the genes from a variety of organisms, it should ultimately be possible to design 'smart' bacteria that will seek out and kill larvae of specific vector mosquitoes. Thus, recombinant bacteria show excellent promise for development and use in operational vector control programs, hopefully within the next few years. documented [5,6]. Vector control is recognized as an effective tool for controlling tropical diseases. Several strategies have been adopted to control these dipteran pests, and to reduce vector-borne diseases. Synthetic insecticides have been effectively used during the past several decades for mosquito-control operations. But the chemical approach has several demerits, such as the development of insecticide resistance, environmental pollution, bio-amplification of contamination of food chain, and harmful effects to beneficial insects. Hence, there has been an increased interest in recent years, in the use of biological agents for mosquito control. Bio-Pesticides as Effective Tools for Mosquito Control In recent years, it has been witnessed, an increased interest in the usage of bio-pesticides as effective tool for mosquito control. Several bio-control agents were screened for their potency, mammalian safety and environmental imp...

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Cloning and Characterization of Two Novel Genes, cry24B and s1orf2, from a Mosquitocidal Strain of Bacillus thuringiensis serovar sotto

Cited by 14 publications

References 19 publications

Search for Cry proteins expressed by Bacillus spp. genomes, using hidden Markov model profiles

Search for Cry proteins expressed by Bacillus spp. genomes, using hidden Markov model profiles

In Vivo Crystallization of Three-Domain Cry Toxins

Current Trends in the Control of Mosquito Vectors by Means of Biological Larvicides

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