Cloning and characterization of two glutathione peroxidase cDNAs from southern bluefin tuna (Thunnus maccoyii)

Thompson, Janene Lee; See, Valene H.L.; Thomas, P.; Schuller, Kathryn A.

doi:10.1016/j.cbpb.2010.04.007

Cited by 24 publications

(15 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The highest GPx activity in hepatopancreas was consistent with the role of hepatopancreas as the main metabolic tissue and the main defense tissue against oxidative stress [17]. The gill is expected to be the main tissue for the generation of ROS [33], which explains why the second highest GPx activity was detected in gills; however, a discrepancy was found between MmeGPx mRNA level and GPx activity in gill. One potential explanation is that some of the GPx in gill may come from hepatopancreas, for MmeGPx was predicted as a secreted protein.…”

supporting

confidence: 60%

Molecular characterization of a glutathione peroxidase gene and its expression in the selected Vibrio-resistant population of the clam Meretrix meretrix

Wang

Huan

Yue

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

supporting

confidence: 60%

Molecular characterization of a glutathione peroxidase gene and its expression in the selected Vibrio-resistant population of the clam Meretrix meretrix

Wang

Huan

Yue

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…However, several fish species, including Japanese conger, Japanese anchovy, chum salmon, Pacific saury, white croaker and marbled sole, contained selenoneine below the level of detection (<0.05 nmol/g tissue) [7]. Selenoproteins, including glutathione peroxidases (GPx) and selenoprotein W, that contain selenium in the selenocysteine form might be present in fish muscles [13,14,15,16,17]. The most important source of selenium in the diets for Japanese is known to be fish [1].…”

Section: Discussionmentioning

confidence: 99%

Selenium Content in Seafood in Japan

Yamashita

Iida

2013

Nutrients

View full text Add to dashboard Cite

Selenium is an essential micronutrient for humans, and seafood is one of the major selenium sources, as well as red meat, grains, eggs, chicken, liver and garlic. A substantial proportion of the total amount of selenium is present as selenium containing imidazole compound, selenoneine, in the muscles of ocean fish. In order to characterize the selenium content in seafood, the total selenium levels were measured in the edible portions of commercially important fish and shellfish species. Among the tested edible portions, alfonsino muscle had the highest selenium levels (concentration of 1.27 mg/kg tissue). High levels of selenium (1.20–1.07 mg/kg) were also found in the salted ovary products of mullet and Pacific herring. In other fish muscles, the selenium levels ranged between 0.12 and 0.77 mg/kg tissue. The selenium levels were closely correlated with the mercury levels in the white and red muscles in alfonsino. The selenium content in spleen, blood, hepatopancreas, heart, red muscle, white muscle, brain, ovary and testis ranged between 1.10 and 24.8 mg/kg tissue in alfonsino.

show abstract

“…They showed conservation of the selenocysteine residue at the active center that was encoded in a TGA codon. GPx1a shares 88, 78, 73, 73, and 70% sequence homology with tilapia GPx1, zebrafish GPx1a [25], zebrafish GPx1b [25], southern bluefin tuna GPx1 [26], and bovine GPx1 [15], respectively (Fig. 3).…”

Section: Enzyme Activitiesmentioning

confidence: 99%

“…3). GPx1b shares 97 and 76% sequence homology with southern bluefin tuna GPx1 [26] and zebrafish GPx1b [25], respectively. The phylogenetic tree of GPx1 in vertebrates shows that fish including Pacific bluefin tuna, zebrafish and Takifugu, but not mammals, possess two genetically distinct types of GPx1, known as GPx1a and GPx1b (Fig.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

et al. 2012

View full text Add to dashboard Cite

Glutathione peroxidase GPx1 was purified from the red muscle of Pacific bluefin tuna Thunnus orientalis and its enzymatic properties were characterized. The enzyme was optimally active at pH 7.4 with a specific activity for hydrogen peroxide and a K m of 6.7 lM. cDNA was also isolated and it contained a predicted open reading frame (ORF) encoding a 188 amino acid protein. The phylogenetic tree shows that fish including Pacific bluefin tuna, pufferfish, and zebrafish, but not mammals, possess two genetically distinct types of GPx1, i.e., GPx1a and GPx1b. The purified enzyme was classified as a fish GPx-1b enzyme on the basis of the phylogenetic tree of the GPx1 family.

show abstract

Cloning and characterization of two glutathione peroxidase cDNAs from southern bluefin tuna (Thunnus maccoyii)

Cited by 24 publications

References 49 publications

Molecular characterization of a glutathione peroxidase gene and its expression in the selected Vibrio-resistant population of the clam Meretrix meretrix

Molecular characterization of a glutathione peroxidase gene and its expression in the selected Vibrio-resistant population of the clam Meretrix meretrix

Selenium Content in Seafood in Japan

Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

Contact Info

Product

Resources

About