Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis

Boe, Lars; Nielsen, Tim T.; Madsen, Søren; Andrup, Lars; Bolander, Gert

doi:10.1016/0147-619x(91)90012-l

Cited by 22 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We introduced the B. cereus tetracycline resistance plasmid pBC16 and the plasmid pAND006 (consisting of the replicon from pTX14-3 inserted into the chloramphenicol resistance vector pBOE335 [6]) into B. thuringiensis subsp. israelensis AND508 (Table 1) by electroporation.…”

Section: Resultsmentioning

confidence: 99%

“…These results indicate that the ability of a plasmid to be mobilized may require only the replication functions. The plasmid pTX14-3 has been shown to replicate, as do most small plasmids in gram-positive bacteria, via the rolling-circle mechanism (6,22), which displays high similarity with the conjugal (24) and some single-stranded bacteriophages of E. coli (35).…”

Section: Discussionmentioning

confidence: 99%

“…First, plasmid pDA72 was electroporated into AND406 and selected by resistance to chloramphenicol. Plasmid pDA72 contains the replicon from plasmid pTX14-2 inserted into the vector pBOE335 (6), which carries the chloramphenicol resistance gene from pC194. Because of incompatibility, strains which contain pDA72 will exclude plasmid pTX14-2.…”

Section: Methodsmentioning

confidence: 99%

“…This subspecies has been reported to contain up to 10 plasmids (8,21). Two of the small plasmids, pTX14-1 and pTX14-3, have been cloned and characterized (3,6,30), a 75-MDa plasmid has been shown to encode the toxin genes (14,21,39,45), and the transfer functions have been ascribed to a 135-MDa plasmid (21) (presumedly the same as pXO16 [36]). …”

mentioning

confidence: 99%

See 3 more Smart Citations

Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation

1993

View full text Add to dashboard Cite

Mobilizations of pBC16 and pAND006, containing the replicon of the Bacillus thuringiensis subsp. israelensis plasmid pTX14-3, between strains of B. thuringiensis subsp. israelensis were examined. Transconjugants appeared after a few minutes and reached a maximum frequency after approximately 2 h. Plasmid pBC16 was mobilized at a frequency approximately 200 times that of pAND006. However, pAND006 was consistently transferred, suggesting that the replicon of pTX14-3 is sufficient to sustain mobilization in B. thuringiensis subsp. israelensis. A specific protease-sensitive coaggregation between strains of B. thuringiensis subsp. israelensis was found to be unambiguously correlated with plasmid transfer. Two aggregation phenotypes, Agr+ and Agr-, were identified in this subspecies. Aggregation disappeared when the optical density of the mating mixture at 600 nm exceeded approximately 1, and it did not reappear upon dilution. Aggregation was shown to involve interactions of cells with opposite aggregation phenotypes, and evidence of a proteinaceous molecule on the surface of the Agr-that is cells involved in aggregation formation is presented. Matings and selection for the presence of two antibiotic resistance plasmids followed by identification of the host cell revealed that mobilization was unidirectional, from the Agr+ cell to the Agr-cell. The aggregation phenotype was found to be transferred with high frequency ("100%) in broth matings, and the appearance of Agr-isolates from Agr+ strains suggested that the loci involved in aggregation formation are located on a plasmid. No excreted aggregation-inducing signals were detected in the supernatant or culture filtrate of either the donor, the recipient, or the mating mixture.Conjugation, the direct transfer of genes from one bacterial cell to another, is often mediated by large conjugative plasmids. These large plasmids are transferred at high frequency, and occasionally chromosomal genes and smaller nonconjugative plasmids are cotransferred by the transfer apparatus of the conjugative plasmids. In gram-negative bacteria many small nonconjugative plasmids can be transferred (mobilized) if an additional self-transmissible plasmid is present in the host (13, 46, 47). Recent reports have described similar mobilizations of small plasmids in gram-positive bacteria (2,5,26,34,40). The first studied and best understood of the conjugative plasmids is the F plasmid, which can replicate in Escherichia coli and certain closely related enteric bacteria. The mechanism of F plasmid-mediated conjugation involves physical contact of the cells and transfer of plasmid DNA brought about by the sex pilus (23). Studies of the gram-positive bacterium Enterococcus (formerly Streptococcus) faecalis suggest a mechanism quite different from that in the better-studied gram-negative systems. Pili do not play any role in conjugation between strains of this organism, but pheromone-induced aggregation between cells has been shown to be a major characteristic (11,15).

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation

1993

View full text Add to dashboard Cite

show abstract

“…Actually, sequences highly similar to motifs 1, 2, and 3 are present in the pTX14-3 origin. Curiously enough, the activity of the sso of pTX14-3 is at least partially resistant to rifampicin, which suggests the existence of a lagging-strand priming mechanism independent of host RNAP (138).…”

Section: The Single-strand Originmentioning

confidence: 99%

Plasmid Rolling-Circle Replication

et al. 2015

View full text Add to dashboard Cite

Plasmids are DNA entities that undergo controlled replication independent of the chromosomal DNA, a crucial step that guarantees the prevalence of the plasmid in its host. DNA replication has to cope with the incapacity of the DNA polymerases to start de novo DNA synthesis, and different replication mechanisms offer diverse solutions to this problem. Rolling-circle replication (RCR) is a mechanism adopted by certain plasmids, among other genetic elements, that represents one of the simplest initiation strategies, that is, the nicking by a replication initiator protein on one parental strand to generate the primer for leading-strand initiation and a single priming site for lagging-strand synthesis. All RCR plasmid genomes consist of a number of basic elements: leading strand initiation and control, lagging strand origin, phenotypic determinants, and mobilization, generally in that order of frequency. RCR has been mainly characterized in Gram-positive bacterial plasmids, although it has also been described in Gram-negative bacterial or archaeal plasmids. Here we aim to provide an overview of the RCR plasmids' lifestyle, with emphasis on their characteristic traits, promiscuity, stability, utility as vectors, etc. While RCR is one of the best-characterized plasmid replication mechanisms, there are still many questions left unanswered, which will be pointed out along the way in this review.

show abstract

Primosome assembly site in Bacillus subtilis.

Bruand¹,

Ehrlich²,

Janniere³

1995

The EMBO Journal

104

View full text Add to dashboard Cite

Corresponding author A single-strand initiation site was detected on the Enterococcus faecalis plasmid pAMP1 by its ability to prevent accumulation of single stranded DNA of a rolling circle plasmid, both in Bacillus subtilis and Staphylococcus aureus. This site, designated ssiA, is located on the lagging strand template, -150 bp downstream from the replication origin. ssiA priming activity requires the DnaE primase, the DnaC replication fork helicase, as well as the products of the dnaB, dnaD and dnal genes of B.subtilis, but not the RNA polymerase. The primase and the replication fork helicase requirements indicate that ssiA is a primosome assembly site.Interestingly, the pAM01 lagging strand synthesis is inefficient when any of the proteins involved in ssiA activity is mutated, but occurs efficiently in the absence of ssiA. This suggests that normal plasmid replication requires primosome assembly and that the primosome can assemble not only at ssiA but also elsewhere on the plasmid. This work for the first time describes a primosome in a Gram-positive bacterium. Involvement of the B.subtilis proteins DnaB, DnaD and DnaI, which do not have any known analogue in Escherichia coli, raises the possibility that primosome assembly and/or function in B.subtilis differs from that in E.coli.

show abstract

Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis

Cited by 22 publications

References 24 publications

Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation

Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation

Plasmid Rolling-Circle Replication

Primosome assembly site in Bacillus subtilis.

Contact Info

Product

Resources

About