Cloning and characterization of three <i>Musca domestica yolk protein</i> genes

White, Neil M.; Bownes, Mary

doi:10.1046/j.1365-2583.1997.00187.x

Cited by 20 publications

(24 citation statements)

References 44 publications

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“…In standard Musca strains, the three yolk protein genes (Mdyp1, Mdyp2, Mdyp3) are transcriptionally repressed in males (White and Bownes 1997). It is thus conceivable that ectopic MdDSX F in males relieves this repression by a direct interaction with the promoter of these genes.…”

Section: Regulation Of Yolk Protein Genes In Muscamentioning

confidence: 99%

Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex

Hediger

Burghardt

Siegenthaler

et al. 2004

Development Genes and Evolution

121

154

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Sex-determining cascades are supposed to have evolved in a retrograde manner from bottom to top. Wilkins' 1995 hypothesis finds support from our comparative studies in Drosophila melanogaster and Musca domestica, two dipteran species that separated some 120 million years ago. The sex-determining cascades in these flies differ at the level of the primary sex-determining signal and their targets, Sxl in Drosophila and F in Musca. Here we present evidence that they converge at the level of the terminal regulator, doublesex ( dsx), which conveys the selected sexual fate to the differentiation genes. The dsx homologue in Musca, Md-dsx, encodes male-specific (MdDSX(M)) and female-specific (MdDSX(F)) protein variants which correspond in structure to those in Drosophila. Sex-specific regulation of Md-dsx is controlled by the switch gene F via a splicing mechanism that is similar but in some relevant aspects different from that in Drosophila. MdDSX(F) expression can activate the vitellogenin genes in Drosophila and Musca males, and MdDSX(M) expression in Drosophila females can cause male-like pigmentation of posterior tergites, suggesting that these Musca dsx variants are conserved not only in structure but also in function. Furthermore, downregulation of Md-dsx activity in Musca by injecting dsRNA into embryos leads to intersexual differentiation of the gonads. These results strongly support a role of Md-dsx as the final regulatory gene in the sex-determining hierarchy of the housefly.

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Section: Regulation Of Yolk Protein Genes In Muscamentioning

confidence: 99%

Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex

Hediger

Burghardt

Siegenthaler

et al. 2004

Development Genes and Evolution

121

154

View full text Add to dashboard Cite

show abstract

“…showed that this fragment contains largely coding sequences, and comparison of three different Musca yp cDNAs (White and Bownes 1997) showed the highest similarity with the Drosophila yp3 gene. To obtain the upstream regulatory regions, we subcloned a 1.6-kb SacI fragment ( Fig.…”

Section: Nutritional Responsementioning

confidence: 91%

“…The amplified library (500,000 plaques) was screened with a 350-bp PCR fragment representing the C-terminal end of the Calliphora CeypB gene (Martinez and Bownes 1994;White and Bownes 1997) radiolabelled by random oligonucleotide-primed synthesis (Feinberg and Volgestein 1984). A 1.6-kb SacI genomic fragment was isolated.…”

Section: Dna Constructsmentioning

confidence: 99%

“…Sequence accession numbers are X97259 for the construct Mupro8 and X97258 for Cepro1. Note that the Musca upstream sequence does not entirely match the equivalent region associated with the yp coding sequences for M. domestica determined from cDNA sequencing (White and Bownes 1997). This is because the libraries used in each case come from different Musca strains.…”

Section: Characterisation Of Cis-regulatory Elements In a Musca Yp Genementioning

confidence: 99%

“…Furthermore, the restriction of yp expression to the adult female fat body and to the follicle cells at specific stages of oogenesis is conserved in all these species (Rina and Savakis 1991;Martinez and Bownes 1994;White and Bownes 1997). It seems possible then that yp genes in all these species share conserved regulatory sequences and mechanisms to achieve a similar spatial and temporal pattern of gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Conservation and divergence in the control of yolk protein genes in dipteran insects

Tortiglione

Bownes

1997

Development Genes and Evolution

Self Cite

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We have investigated the conservation of regulatory elements for sex- and tissue-specific gene expression in three dipteran species, Drosophila melanogaster, Musca domestica and Calliphora erythrocephala, using the yolk protein (yp) genes. Yolk proteins of the fruitfly, medfly, housefly and blowfly are very well conserved both in their sequence and their expression in ovarian follicle cells and in fat bodies of adult females. Furthermore, yp regulation by both hormonal and nutritional factors shows similar features in all four species. To study conservation of yp regulation in dipteran insects, we tested 5' flanking regions from one Musca yp gene and one Calliphora yp gene for enhancer functions in D. melanogaster. Two fragments of 823 and 1046 bp isolated from Musca and Calliphora yp genes, respectively, are able to direct correct expression of a reporter gene in the ovarian follicle cells of transformed Drosophila at specific stages during oogenesis. Surprisingly, these enhancers do not confer sex-specific reporter gene expression in the fat body, as expression was found in both sexes of the transformed flies. None-the-less by in vitro DNA/protein interaction assays, a 284-bp DNA region from the Musca yp enhancer was able to bind the Drosophila DOUBLESEX (DSX) protein, which in D.melanogaster confers sex-specific expression of yp. We speculate that the sex-determining pathway is not directly involved in yp regulation in Musca or Calliphora adult females, but depends instead on hormonal controls to achieve sex-specific expression of yp genes in the adult.

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Novel Morphological and Physiological Aspects of Insect Eggs

Trougakos¹,

Margaritis²

2003

Chemoecology of Insect Eggs and Egg Deposition

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Cloning and characterization of three Musca domestica yolk protein genes

Cited by 20 publications

References 44 publications

Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex

Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex

Conservation and divergence in the control of yolk protein genes in dipteran insects

Novel Morphological and Physiological Aspects of Insect Eggs

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