Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa

Abstract

ABSTRACT. Eruca vesicaria subsp sativa is one of the most tolerant Cruciferae species to drought, and dehydration-responsive elementbinding protein 2A (DREB2A) is involved in responses to salinity, heat, and particularly drought. In this study, a gene encoding EvDREB2A was cloned and characterized in E. vesicaria subsp sativa. The fulllength EvDREB2A cDNA sequence contained a 388-bp 5'-untranslated region (UTR), a 348-bp 3'-UTR, and a 1002-bp open reading frame that encoded 334 amino acid residues. The theoretical isoelectric point of the EvDREB2A protein was 4.80 and the molecular weight was 37.64 kDa. The genomic sequence of EvDREB2A contained no introns. Analysis using SMART indicated that EvDREB2A contains a conserved AP2 domain, similar to other plant DREBs. Phylogenetic analysis revealed that EvDREB2A and DREB2As from Brassica rapa, Eutrema salsugineum, Arabidopsis thaliana, Arabidopsis lyrata, and Arachis hypogaea formed a small subgroup, which clustered with DREB2Bs from A. lyrata, A. thaliana, Camelina sativa, and B. rapa to form a larger subgroup. EvDREB2A is most closely related to B. rapa DREB2A, followed by DREB2As from E. salsugineum, A. thaliana, A. hypogaea, and A. lyrata. A quantitative real-time polymerase chain reaction indicated that EvDREB2A expression was highest in the leaves, followed by the roots and hypocotyls, and was lowest in the flower buds. EvDREB2A could be used to improve drought tolerance in crops.

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

“…6). Upregulation of the Dehydration-Responsive Element -Binding protein 2A (DREB2A) can activate the various genes related to stress tolerance in different plant species [45]. It has also been reported that over-expression of OsTIFY11 (located in Chr3:M-QTL1, up-regulated in the shoot and root) increased the tolerance to salinity stress through the Jasmonic Acid (JA) signaling and through modulating the potassium homeostasis (Table S6, Fig.…”

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

“…HSFA6B (located in Chr1:M-QTL3, up-regulated in the seedlings) acts as a positive regulator downstream of Abscisic Acid (ABA) signaling directly bound to the promoter of Dehydration-Responsive Element-Binding (DREB) and increasing its expression (Table S6, Fig.6). Upregulation of the Dehydration-Responsive Element -Binding protein 2A (DREB2A) can activate the various genes related to stress tolerance in different plant species [45]. It has also been reported that overexpression of OsTIFY11 (located in Chr3:M-QTL1, up-regulated in the shoot and root) increased the tolerance to salinity stress through the Jasmonic Acid (JA) signaling and through modulating the potassium homeostasis (Table S6, Fig.6) [46].…”

Determination of Lentil (Lens culinaris M.) DEHYDRATION RESPONSIVE ELEMENT-BINDING2A (DREB2A) Gene Expression under Drought Stress Conditions

“…saatlere göre önemli bir farkın oluşmadığı bildirilmiştir (Liu et al 2008). Eruca vesicaria subsp sativa'da ise, EvDREB2A geninin en yüksek ifade seviyesinin yapraklarda, en düşük ise çiçek tomurcuklarında olduğunu bildirmişlerdir (Huang et al 2016). DREB2A transkripsiyon faktörünün aşırı ifade edilmesi ile, bitkilerde kuraklık stresine karşı önemli dayanıklılıkların sağlandığını bildiren çalışmalar da bulunmaktadır (Sakuma, 2006a(Sakuma, , 2006bQin et al 2007;Agarwal et al 2010;Chen et al 2016).…”