Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa

Legerton, Timothy L.; Yanofsky, Charles

doi:10.1016/0378-1119(85)90306-3

Cited by 103 publications

(46 citation statements)

References 22 publications

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“…The weak molecular hybridization intensity of RDN1 form II and HIS4 probes from S. cerevisiae to DNA from Zygosaccharomyces fermentati reveal the relative large genetic distance between S. cerevisiae and Z. fermentati. That molecular hybridization was possible is probably due to both the redundancy of RDN1 genes (54) and the conservation of coding sequences in the RDN1 and HIS4 genes throughout the Fungi (33,52,60). Large genetic differentiation among the species in the genus Saccharomyces had already been uncovered by WINGE and LAUSTSEN, when they had great difficulties in producing viable offspring from the hybrid between S. cerevisiae and S. vatidus (now S. uvarum) (63).…”

Section: Discussionmentioning

confidence: 99%

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

Pedersen

1986

Carlsberg Res. Commun.

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30 bottom fermenting strains, 13 hybrid lager strains, 11 top fermenting strains. In addition, 24 other yeast species and strains have been analysed in this study. Four DNA regions have been monitored by restriction endonuclease fragment pattern polymorphisms. The regions analysed are: RDN1 (encoding cytosolic ribosomal RNA molecules) located on chromosome XII in S. cerevisiae, HIS4 (histidine 4) and LEU2 (leucine 2) both located on chromosome III and the Ty elements which are distributed at different positions in the genome ofS. cerevisiae. Seven Ty fragment patterns have been detected in the bottom fermenting strains. Patterns I, II, IIa, lib, and llc are present in lager strains while pattern III and IV are found in S. carlsbergensis bottom fermenting strain No. I and in S. monacensis, respectively. Hybridization of the Tyl p14 probe from S. cerevisiae to OFAGE separated chromosomes from bottom fermenting strains show that at least five Ty elements are present in the genomes of the lager strains, at least five in S. carlsbergensis bottom fermenting strain No. I and at least three in S. monacensis. From the restriction fragment patterns and the karyotypes of the bottom fermenting strains it is suggested that these strains are closely related and different from the S. bayanus, S. pastorianus, and S. uvarum group as well as from the S. cerevisiae strains. In spite of their differences in Ty element patterns the lager strains are so homogenous that most likely they all originate from a single strain which is related to S. carlsbergensis and S. monacensis type strains. In contrast to the bottom fermenting strains there is a large variability among the top fermenting strains, especially with regard to the LEU2 gene and Ty elements. It seems that S. bayanus, S. inusitatus, S. pastorianus, S. validus, and S. uvarum are closely related, and none of the studied strains from these taxons contained DNA which hybridized to the Tyl probe from S. cerevisiae. This is a clear difference from the DNA of bottom fermenting strains which consistently hybridized strongly to the Tyl probe.Abbreviations: kb = kilo base pairs; OFAGE = orthogonal field alternation gel electrophoresis; SSC = 0.15 M-NaCI, 15 mM-Na citrate; TBE = 0.089 M-Tris-borate, 0.089 M-boric acid, 0.002 M-EDTA; Tris = tris-(hydroxymethyl)-amino methane Springer-Verlag

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Section: Discussionmentioning

confidence: 99%

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

Pedersen

1986

Carlsberg Res. Commun.

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“…Plasmid DNA was isolated by the alkaline lysis method of Birnboim and Doly (2). Plasmids containing Neurospora genes were used as probes of chromosomal DNA blots: pACqa-2A qa-2 (linkage group VII [LG VII]) (W. Schneider and C. Yanofsky, unpublished data), pAR201 arg-2 (LG IV) (M. J. Orbach, unpublished data), pNC2 trp-J (LG III) (18), pDB1 trp-3 (LG IIR) (D. Burns and C. Yanofsky, unpublished data) pFB6 pyr-4 (LG IIL) (3), pBT3 tub-2 (LG VI) (14), and pNH60 his-3 (LG I) (10). The tub-2 locus is also referred to as Bml (16).…”

Section: Methodsmentioning

confidence: 99%

An electrophoretic karyotype of Neurospora crassa.

et al. 1988

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A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

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“…In Lactococctls lactis (Delorme e t al., 1992), the relative order of the genes is the same as in enteric bacteria, but the operon contains additional ORFs of unknown function. In Streptomyces coelicolor (Limauro et al, 1990) several genes are located in a main cluster, while other genes have been found in unlinked loci of the chromosome. A main cluster that does not contain all the genes is also found in Bacillus sabtilis (Henner e t al., 1986) and Aaospirillum brasilense (Fani e t al., 1993).…”

Section: Introductionmentioning

confidence: 99%

“…A main cluster that does not contain all the genes is also found in Bacillus sabtilis (Henner e t al., 1986) and Aaospirillum brasilense (Fani e t al., 1993). In Saccharomyces cerevisiae (Donhaue e t al., 1982) and Negrospora crassa (Legerton & Yanofsky, 1986) the histidine genes are scattered over different chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Cloning of the Rhodobacter sphaeroides hisI gene: unifunctionality of the encoded protein and lack of linkage to other his genes

et al. 1996

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The Rhodobacter sphaeroides 2.4.1 his/ gene, which encodes a phosphoribosyl-AMP-cyclohydrolase that catalyses the third step in the histidine biosynthetic pathway, has been isolated from a genomic library of this phototrophic bacterium by complementation of an Escherichia coli his/ mutant. Analysis of the nucleotide sequence of the R. sphaeroides his! gene reveals that it encodes a deduced product of 119 aa with a predicted molecular mass of 13.4 kDa. In contrast to the situation in E. coli, the R. sphaeroides his/ gene encodes a unifunctional protein and it is not linked to the his€ gene. The absence of a single histidine operon like that of E. coli was confirmed by PFGE experiments and complementation analysis of a R. sphaeroides his/ mutant that was constructed by marker exchange. The location of his/ in the R. sphaeroides genome has been determined to be at map co-ordinate 2275 +20 of chromosome 1.

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Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa

Cited by 103 publications

References 22 publications

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

DNA sequence polymorphisms in the genus saccharomyces III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains

An electrophoretic karyotype of Neurospora crassa.

Cloning of the Rhodobacter sphaeroides hisI gene: unifunctionality of the encoded protein and lack of linkage to other his genes

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