Cloning and Characterization of the Replicon of the Nocardia italica Plasmid, pNI100

Liu, Yu-Tien; Su, Chin Cheng; Lee, Chao-Huer; Sui, Meng-Jiun; Chang, Yu‐Hsin; Lin, Wei‐Chun; Wu, Wen-Tung; Chen, Chien-Yuan

doi:10.1006/plas.1999.1445

Cited by 6 publications

(6 citation statements)

References 21 publications

(22 reference statements)

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“…The two replication proteins (RepA and RepB) found in most small plasmids of Nocardia, as well as those of the closely related Rhodococcus, resemble replication proteins of the theta-type replicating Mycobacterium plasmid pAL5000 (111,177,241). The replication protein of Nocardia plasmid pNI100 (308a) resembles the rolling-circle replication proteins encoded by the Streptomyces plasmids pIJ101 and pSG5 (238,277).…”

Section: Extrachromosomal Dna Elements In Nocardiamentioning

confidence: 98%

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Ventura

Canchaya

Tauch

et al. 2007

Microbiol Mol Biol Rev

910

638

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SUMMARY Actinobacteria constitute one of the largest phyla among Bacteria and represent gram-positive bacteria with a high G+C content in their DNA. This bacterial group includes microorganisms exhibiting a wide spectrum of morphologies, from coccoid to fragmenting hyphal forms, as well as possessing highly variable physiological and metabolic properties. Furthermore, Actinobacteria members have adopted different lifestyles, and can be pathogens (e.g., Corynebacterium, Mycobacterium, Nocardia, Tropheryma, and Propionibacterium), soil inhabitants (Streptomyces), plant commensals (Leifsonia), or gastrointestinal commensals (Bifidobacterium). The divergence of Actinobacteria from other bacteria is ancient, making it impossible to identify the phylogenetically closest bacterial group to Actinobacteria. Genome sequence analysis has revolutionized every aspect of bacterial biology by enhancing the understanding of the genetics, physiology, and evolutionary development of bacteria. Various actinobacterial genomes have been sequenced, revealing a wide genomic heterogeneity probably as a reflection of their biodiversity. This review provides an account of the recent explosion of actinobacterial genomics data and an attempt to place this in a biological and evolutionary context.

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Section: Extrachromosomal Dna Elements In Nocardiamentioning

confidence: 98%

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Ventura

Canchaya

Tauch

et al. 2007

Microbiol Mol Biol Rev

910

638

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“…Experimental evidence shows that replication of Rhodococcus plasmid pRE8424 is in a type of RCR (Nakashima & Tamura, 2004). Replication proteins of Rhodococcus plasmid pAN12 and Nocardia plasmid pNI100 resemble the Rep of Streptomyces RCR plasmids pIJ101 or pSG5 (Liu et al, 2000;Kostichka et al, 2003;Nakashima & Tamura, 2004), indicating that plasmids pAN12 and pNI100 are also RCR. The Rep of 80 kb Rhodococcus large plasmid p33701 (Zheng et al, 1997;Takai et al, 2000) displays only homology with the Rep of plasmid pC1 that we identified here, suggesting that the replication of plasmid pC1 may be in a type of y.…”

Section: Discussionmentioning

confidence: 99%

“…The two Reps (RepA and RepB) of most Rhodococcus small plasmids, including pRC4, pB264, pN30, pKA22 and pFAJ2600, resemble the Reps of Mycobacterium plasmid pAL5000 (De Mot et al, 1997;Kulakov et al, 1997;Hirasawa et al, 2001;Lessard et al, 2004), and the Rep of 100 kb Rhodococcus large plasmid pSOX (Denis-Larose et al, 1998) resembles the Rep of several Mycobacterium plasmids (e.g. Replication proteins of Rhodococcus plasmid pAN12 and Nocardia plasmid pNI100 resemble the Rep of Streptomyces RCR plasmids pIJ101 or pSG5 (Liu et al, 2000;Kostichka et al, 2003;Nakashima & Tamura, 2004), indicating that plasmids pAN12 and pNI100 are also RCR. replicating in Mycobacterium).…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that 27 of 87 clinically identified strains of Nocardia harbored one to four circular plasmids ranging in size from o 8 to 4 50 kb (Provost et al, 1996). The origin of DNA replication of circular plasmid pNI100 of Nocardia italica has been identified and its replication protein resembles the Rep of the rolling-circle replication (RCR) Streptomyces circular plasmid pSG5 (Liu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Replication and inheritance ofNocardiaplasmid pC1

Shen

Fang

et al. 2006

FEMS Microbiology Letters

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Nocardia sp. C-14-1, isolated from acrylic fiber wastewater, can degrade long-chain alkanes and succinonitrile efficiently. Here we report the characterization of an indigenous plasmid pC1. The overall nucleotide sequence of pC1 consisted of 5841 bp. The five ORFs, encoding a DNA recombinase, replication protein (Rep(pC1)) and three proteins of unknown function, were predicted on pC1. The Rep(pC1) displayed its homology with the Rep of Rhodococcus large plasmid p33701, suggesting a theta type of replication. An Escherichia coli plasmid (containing the single rep(pC1) gene) propagated autonomously in low copy number in Nocardia or Rhodococcus, suggesting that rep(pC1) was an essential gene for plasmid replication. The plasmid (containing the single rep(pC1) gene) presented as inheritance unstable hints that other pC1 loci were required for the stable inheritance of plasmids. By comparison of the plasmid-borne Rep proteins, we classify Rhodococcus or Nocardia plasmids into four groups.

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“…Indigenous circular plasmids have been detected among Nocardia species [5−7], but few are characterized. The replication extragenic palindromic (Rep) protein of plasmid pNI100 of Nocardia italica resembles that of the rollingcircle-replication (RCR) plasmid pSG5 of Streptomyces, and a shuttle vector of pNI100 derivative can propagate in Streptomyces [8].…”

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confidence: 99%

Characterization of <italic>Nocardia</italic> Plasmid pXT 107

Xia

Tian

Zhang

et al. 2006

ABBS

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Nocardia, Rhodococcus and Streptomyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G+C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia. The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G+C content, and encoded one replication extragenic palindromic (Rep) and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI100 of Nocardia, pRE8424 of Rhodococcus and pIJ101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing the rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.

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Cloning and Characterization of the Replicon of the Nocardia italica Plasmid, pNI100

Cited by 6 publications

References 21 publications

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Replication and inheritance ofNocardiaplasmid pC1

Characterization of <italic>Nocardia</italic> Plasmid pXT 107

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Cloning and Characterization of the Replicon of the Nocardia italica Plasmid, pNI100

Cited by 6 publications

References 21 publications

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Replication and inheritance ofNocardiaplasmid pC1

Characterization of &lt;italic&gt;Nocardia&lt;/italic&gt; Plasmid pXT 107

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Characterization of <italic>Nocardia</italic> Plasmid pXT 107