Cloning and characterization of the executioner caspases 3, 6, 7 and Hsp70 in hyperthermic Atlantic salmon (Salmo salar) embryos

Takle, Harald; McLeod, Anette; Andersen, Øivind

doi:10.1016/j.cbpb.2006.02.006

Cited by 66 publications

(51 citation statements)

References 45 publications

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“…Extensive data mining of fish genomes has revealed a range of orthologs of mammalian caspases in both zebrafish and other nonmodel fish species such as salmon [25,46]. In rainbow trout little is known about the structure and function of caspases, except for the study of Laing et al [24] which showed the immunoregulatory role of trout caspase-6.…”

Section: Discussionmentioning

confidence: 99%

“…In trout, only caspase-6 has been reported previously [24], although in salmon caspase-3, -6, and -7 have also been reported [25]. To identify other caspase genes in trout, a tblastn search using salmon caspase protein sequences as baits was performed against the rainbow trout ESTs in the Computational Biology and Functional Genomic Laboratory database.…”

Section: Database Mining Of Trout Caspase Genesmentioning

confidence: 99%

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Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Chang

Wang

Nie

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Database Mining Of Trout Caspase Genesmentioning

confidence: 99%

Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Chang

Wang

Nie

et al. 2011

Fish & Shellfish Immunology

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“…These observations at 8°C are not really surprising since the number of studies concerning the influence of cold stress on gene expression patterns in fish is growing (Donaldson et al 2008). In addition, the cold-induced expression of HSPs in salmonids has already been demonstrated in the literature, including in Salmo salar embryos (Takle et al 2006) and in rainbow trout cell lines (Yamashita et al 1996). Besides HSP70, the well-characterized cold marker cold-inducible RNA-binding protein (CIRBP) is topregulated at moderate hypothermia in BORN (3.6-to 8.3-fold) and imported trout (4.1-to 5.5-fold), as represented by three different probes on the microarray (Table 2, Fig.…”

Section: Exposure To Mild Heat and Cold Triggers Common Stressmentioning

confidence: 91%

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

et al. 2015

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Seasonal water temperatures can be stressful for fish in aquaculture and can therefore negatively influence their welfare. Although the kidney is the crucial organ associated with the primary stress response, knowledge about the stress-modulated kidney transcriptome in salmonids is limited. In the present study, we used a comparative microarray approach to characterize the general gene expression profiles of rainbow trout trunk kidney after a 2-week acclimation to mild heat (23 °C) and cold stress (8 °C). Hypothesizing that local adaptation influences stress performance, we aimed to identify differences in the temperature-induced gene expression in the regional trout strain BORN, in addition to a common imported strain. Moderate temperature challenge provoked typical stress response clusters, including heat-shock proteins or cold-inducible factors, in addition to altered energy metabolism in trout kidney. Mild cold, in particular, enhanced renal protein degradation processes, as well as mRNA and protein synthesis, while it also triggered fatty acid biosynthesis. Mild heat led to cytoskeleton-stabilizing processes and might have facilitated cell damage and infection. Furthermore, both breeding lines used different strategies for energy provision, cellular defense, and cell death/survival pathways. As a main finding, the genes involved in energy provision showed generally higher transcript levels at both temperatures in BORN trout compared to imported trout, indicating adjusted metabolic rates under local environmental conditions. Altogether, this study provides a general overview of stress-induced transcriptional patterns in rainbow trout trunk kidney, in addition to identifying genes and networks that contribute to the robustness of the BORN strain. Our analyses suggest SERPINH1 and CIRBP as general marker genes for heat stress and cold stress in trout, respectively.

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“…Primers for hsp70 were designed from Atlantic salmon (Salmo salar) sequence as described by Takle et al (2006). Primers for nkaα1a and ef1α were designed from respectively rainbow trout and Atlantic salmon sequences as described by Kiilerich et al (2007).…”

Section: −Ctef1αmentioning

confidence: 99%

Metabolic fates and effects of nitrite in brown trout under normoxic and hypoxic conditions: blood and tissue nitrite metabolism and interactions with branchialNOS,Na+/K+-ATPaseandhsp70expression

Frank

Gerber

Hansen

et al. 2015

Journal of Experimental Biology

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Nitrite secures essential nitric oxide (NO) bioavailability in hypoxia at low endogenous concentrations, whereas it becomes toxic at high concentrations. We exposed brown trout to normoxic and hypoxic water in the absence and presence of added ambient nitrite to decipher the cellular metabolism and effects of nitrite at basal and elevated concentrations under different oxygen regimes. We also tested hypotheses concerning the influence of nitrite on branchial nitric oxide synthase (NOS), Na + /K + -ATPase (nka) and heat shock protein (hsp70) mRNA expression. Basal plasma and erythrocyte nitrite levels were higher in hypoxia than normoxia, suggesting increased NOS activity. Nitrite exposure strongly elevated nitrite concentrations in plasma, erythrocytes, heart tissue and white muscle, which was associated with an extensive metabolism of nitrite to nitrate and to iron-nitrosylated and S-nitrosated compounds. Nitrite uptake was slightly higher in hypoxia than normoxia, and high internal nitrite levels extensively converted blood hemoglobin to methemoglobin and nitrosylhemoglobin. Hypoxia increased inducible NOS (iNOS) mRNA levels in the gills, which was overruled by a strong inhibition of iNOS expression by nitrite in both normoxia and hypoxia, suggesting negative-feedback regulation of iNOS gene expression by nitrite. A similar inhibition was absent for neuronal NOS. Branchial NKA activity stayed unchanged, but mRNA levels of the nkaα1a subunit increased with hypoxia and nitrite, which may have countered an initial NKA inhibition. Nitrite also increased hsp70 gene expression, probably contributing to the cytoprotective effects of nitrite at low concentrations. Nitrite displays a concentrationdependent switch between positive and negative effects similar to other signaling molecules.

show abstract

Cloning and characterization of the executioner caspases 3, 6, 7 and Hsp70 in hyperthermic Atlantic salmon (Salmo salar) embryos

Cited by 66 publications

References 45 publications

Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

Metabolic fates and effects of nitrite in brown trout under normoxic and hypoxic conditions: blood and tissue nitrite metabolism and interactions with branchialNOS,Na+/K+-ATPaseandhsp70expression

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