Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)

Li, L; Li, N; Song, Shufeng; Li, Y X; Xia, X. Y.; Fu, Xiqin; Chen, G H; Deng, Huafeng

doi:10.4238/2014.may.23.13

Cited by 17 publications

(16 citation statements)

References 30 publications

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“…In this study, we identified MePMP3-2 by searching sequences with similarity to the rice gene OsRCI2-5. OsRCI2-5 is a stress-responsive gene of rice and, when over-expressed, greatly enhances drought tolerance of transgenic rice (Li et al, 2014). The results of our studies show that MePMP3-2 is induced by salt and drought stresses.…”

Section: Introductionmentioning

confidence: 57%

“…In this study, a gene homologous to the stress-inducible OsRCI2-5 (Li et al, 2014), MePMP3-2, was isolated for the first time from cassava and its expression was shown to increase in response to salt and drought stresses. Like OsRCI2-5, the predicted MePMP3-2 protein was found to belong to the PMP3 family of small membrane proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Results from our previous research showed that rice OsRCI2-5 (AK070872) is a drought resistance gene (Li et al, 2014). To identify homologous genes in cassava that could have a similar role, we performed a BLAST of the OsRCI2-5 sequence against the Phytozome database (http:// phytozome.jgi.doe.gov/pz/portal.html#).…”

Section: Mepmp3-2 Encodes a Pmp3 Family Proteinmentioning

confidence: 99%

“…expasy.org/protparam/). The BLASTx revealed MePMP3-2 to be similar to numerous plant PMP3s including several encoded by stress-inducible genes such as LeESI3, rice OsRCI2-5, OsLti6a/b, R1G1B) (Li et al, 2014), AcPMP3-1/2, AtRCI2A/B/D/F (Medina et al, 2007), PutPMP3-1/2, and ZmPMP3-2/3/4 (Fu et al, 2012) (Figure 1A and B). In particular, these polypeptides share the…”

Section: Mepmp3-2 Encodes a Pmp3 Family Proteinmentioning

confidence: 99%

“…In plants, multiple structural variants of PMP3 proteins exist, which are encoded by multigene families. Among the abiotic stress-induced plant PMP3 genes are LeESI3 from wheatgrass (Lophopyrum elongatum), OsLti6a/b, and R1G1B from rice (Li et al, 2014), AcPMP3-1 from sheepgrass (Aneurolepidium chinense), AtRCI2A/B/D/F from Arabidopsis (Arabidopsis thaliana) (Medina et al, 2007), PutPMP3-1/-2 from alkali grass (Puccinellia tenuiflora), and ZmPMP3-2/3/4 from corn (Zea mays) (Fu et al, 2012). There is some evidence for functional specialization of plant PMP3s and of their participation in stress responses being effected in different ways.…”

Section: Introductionmentioning

confidence: 99%

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Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses

Yu¹,

Cui²,

Ren³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Plasma membrane proteolipid 3 (PMP3) is a class of small hydrophobic proteins found in many organisms including higher plants. Some plant PMP3 genes have been shown to respond to abiotic stresses and to participate in the processes of plant stress tolerance. In this study, we isolated the cassava (Manihot esculenta Crantz) MePMP3-2 gene and functionally characterized its role in tolerance to abiotic stress by expressing it in rice (Oryza sativa L.). MePMP3-2 encodes a 77-amino acid protein belonging to a subgroup of plant PMP3s that have long hydrophylic C-terminal tails of unknown function. In silico analysis and co-localization studies indicated that MePMP3-2 is a plasma membrane protein with two transmembrane domains, similar to other PMP3s. In cassava leaves, MePMP3-2 expression was up-regulated by salt and drought stresses. Heterologous constitutive expression of MePMP3-2 in rice did not alter plant growth and development but increased tolerance to salt and drought stresses. In addition, under stress conditions MePMP3-2 transgenic plants accumulated less malondialdehyde, had increased levels of proline, and exhibited greater up-regulation of the stress-related genes OsProT and OsP5CS, but led to only minor changes in OsDREB2A and OsLEA3 expression. These findings indicate that MePMP3-2 may play an important role in salt and drought stress tolerance in transgenic rice.

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Section: Introductionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Mepmp3-2 Encodes a Pmp3 Family Proteinmentioning

confidence: 99%

Section: Mepmp3-2 Encodes a Pmp3 Family Proteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses

Yu¹,

Cui²,

Ren³

et al. 2016

Genet. Mol. Res.

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Application of image-based phenotyping tools to identify QTL for in-field winter survival of winter wheat (Triticum aestivum L.)

et al. 2019

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Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

Rocha

2015

Planta

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RCI2 / PMP3 s participate in abiotic stress responses and impact the expression of other genes. Their multifunctionality is determined by differential expression and by distinct activities of their structurally different proteins. In plants, RCI2/PMP3 genes, which encode small membrane proteins of the PMP3 family, are closely associated with abiotic stress responses. Their involvement in mediating stress tolerance is supported by genetic evidence and overexpression studies. RCI2/PMP3s occur as multigenes in plant genomes and their encoded proteins belong to distinct and conserved structural groups. In addition, different isoforms appear to be targeted to the plasma membrane or to distinct endomembrane compartments in cells. Several studies have revealed that RCI2/PMP3 proteins participate in cell ion homeostasis, and in regulation of membrane stability and polarization. They also appear to potentiate plant transcriptional responses to abiotic stresses. However, their mechanisms of action remain unknown. This paper reviews the current knowledge of the multiple roles of plant RCI2/PMP3 genes resulting from their differential expression under normal and stress conditions. The structural diversity of RCI2/PMP3 proteins is analyzed and evidence supporting their functional specialization and possible activity mechanisms is examined. Finally, strategies are discussed for exploiting new and established technologies to overcome the difficulties posed by the multigene status of RCI2s and the integral membrane character of their proteins, enabling the probing of their individual functions and collective significance.

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Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)

Cited by 17 publications

References 30 publications

Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses

Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses

Application of image-based phenotyping tools to identify QTL for in-field winter survival of winter wheat (Triticum aestivum L.)

Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

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