Cloning and characterization of the pheromone biosynthesis activating neuropeptide receptor gene in Spodoptera littoralis larvae

Zheng, Lei; Lytle, Christian; Njauw, Ching‐Ni Jenny; Altstein, Miriam; Martins‐Green, Manuela

doi:10.1016/j.gene.2006.12.025

Cited by 47 publications

(27 citation statements)

References 50 publications

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“…The production of sex pheromone is mainly dependent on an endocrine signal of PBAN, whose molecular event is initiated by binding to PBANr (Matsumoto et al, 2009;Rafaeli, 2009). To date, PBAN receptors have been identified and characterized from various moths (Hull et al, 2004;Rafaeli et al, 2007;Zheng et al, 2007;Kim et al, 2008). The family of PBAN receptors can be classified into two groups depending on the existence of long C-terminus tail: the first PBAN receptor (Hez-PBANr) identified from H. zea induces extracellular Ca 2þ influx and subsequent increase of cAMP level when challenged with PBAN (Choi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

Lee

Shrestha

Kim

et al. 2011

Insect Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

Lee

Shrestha

Kim

et al. 2011

Insect Biochemistry and Molecular Biology

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“…Utilizing the relationship of structural and functional similarity of ligands and receptors, we identified the first PBAN receptor (PBAN-R) from pheromone glands of Helicoverpa zea female moths, which was confirmed to belong to the rhodopsin family of GPCRs with 7-transmembrane domains (7-TM) (Choi et al, 2003). Subsequently, identification of PBAN-Rs have been reported from other moths including Bombyx mori (Hull et al, 2004), H. armigera (Choi et al, 2005), Spodoptera littoralis (Zheng et al, 2006) and Plutella xylostella (Lee and Boo, 2006), with receptors showing very similar sequences.…”

Section: Introductionmentioning

confidence: 94%

Role of extracellular domains in PBAN/pyrokinin GPCRs from insects using chimera receptors

Choi

Fuerst

Rafaeli

et al. 2007

Insect Biochemistry and Molecular Biology

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“…Pheromone biosynthesis activating neuropeptide receptors that have C-terminal sequences more similar to HelzePBANR than to BommoPBANR have since been cloned from other moth species (Rafaeli et al, 2007; Zheng et al, 2007; Cheng et al, 2010; Lee et al, 2011). However, multiple PBANR subtypes have recently been identified in the tobacco budworm Heliothis virescens and the tobacco hornworm Manduca sexta (Kim et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

et al. 2012

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Sex pheromone production in most moths is initiated following pheromone biosynthesis activating neuropeptide receptor (PBANR) activation. PBANR was initially cloned from pheromone glands (PGs) of Helicoverpa zea and Bombyx mori. The B. mori PBANR is characterized by a relatively long C-terminus that is essential for ligand-induced internalization, whereas the H. zea PBANR has a shorter C-terminus that lacks features present in the B. mori PBANR critical for internalization. Multiple PBANRs have been reported to be concurrently expressed in the larval CNS of Heliothis virescens. In the current study, we sought to examine the prevalence of multiple PBANRs in the PGs of three moths and to ascertain their potential functional relevance. Multiple PBANR variants (As, A, B, and C) were cloned from the PGs of all species examined with PBANR-C the most highly expressed. Alternative splicing of the C-terminal coding sequence of the PBAN gene gives rise to the variants, which are distinguishable only by the length and composition of their respective C-terminal tails. Transient expression of fluorescent PBANR chimeras in insect cells revealed that PBANR-B and PBANR-C localized exclusively to the cell surface while PBANR-As and PBANR-A exhibited varying degrees of cytosolic localization. Similarly, only the PBANR-B and PBANR-C variants underwent ligand-induced internalization. Taken together, our results suggest that PBANR-C is the principal receptor molecule involved in PBAN signaling regardless of moth species. The high GC content of the C-terminal coding sequence in the B and C variants, which makes amplification using conventional polymerases difficult, likely accounts for previous “preferential” amplification of PBANR-A like receptors from other species.

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Cloning and characterization of the pheromone biosynthesis activating neuropeptide receptor gene in Spodoptera littoralis larvae

Cited by 47 publications

References 50 publications

RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

Role of extracellular domains in PBAN/pyrokinin GPCRs from insects using chimera receptors

Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

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