Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp.

Barve, M.P.; Arie, Tsutomu; Salimath, S. S.; Muehlbauer, F. J.; Peever, Tobin L.

doi:10.1016/s1087-1845(03)00015-x

Cited by 105 publications

(117 citation statements)

References 51 publications

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“…The null hy poth e sis is that if A.pinodella Mat genes are able to func tion in A.rabiei this is ev i dence of abil ity to out cross ing un der field con di tion . Barve et al (2003) in phylo gen etic anal y ses based on ITS se quence data have re vealed that P.pinodella and A.pinodes are very closely re lated. On the other hand A,pinodes could not be long to ge nus Mycosphaerella due to phylo gen etic dis tance to other Mycosphaerella spe cies like M.graminicola, M.citri and M.fijiensis.…”

Section: Discussionmentioning

confidence: 99%

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

Furgał-Węgrzycka¹,

Węgrzycki²

2009

Plant Breeding and Seed Science

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The genotypic pop u la tions struc ture of Ascochyta pinodes and Phoma pinodella was de ter mined across a hi er ar chy of spa tial and tem po ral scale by us ing veg e ta tive in com pat i bil ity sys tem as phenotypic ge netic marker. Com po nents of ge netic vari a tion within and be tween pop u la tions in cluded the fol low ing val ues: in dices of di ver sity H', rich ness (g), in di ces of even ness E 1 , E 2 and E 5, spatio-tem po ral anal y sis of genotypic varia tion (H T and H S val ues), pairwise com par i sons of ge no type di ver sity, dis tri bu tion of VCGs across spatio-tem po ral scale (G ST value) and anal y sis of vari ance cal cu lated on VCGs ge no type fre quen cies within pop u la tions. It has been found that genotypic pop u la tions struc ture of Ascochyta pinodes and Phoma pinodella be long to mixed pop u la tion struc ture clonal and re com bin ing. Three re gional pop u la tions of Ascochyta pinodes and Phoma pinodella dif fered sig nif i cantly in both of VCGs ge no type fre quen cies and their dis tri bu tion on a spatio-tem po ral scale in di cat ing on the low or mod er ate de gree of ge no type mi gra tion be tween re gions of Po land.Keywords: Mycosphaerella (Didymella) pinodes anamorph Ascochyta pinodes, Phoma pinodella (teleomorph Didymella), genotypic pop u la tion struc ture, com po nents the ge netic vari a tion of pop u la tion, VCG fre quency, spa tial and tem po ral VCG diversity INTRODUCTIONAscochyta blight of pea is caused pri mar ily by Ascochyta pinodes (Berk et Blox) Jones teleomorph Mycosphaerella pinodes (Berk et Blox) Vesterg = Didymella pinodes, Pertrak and Phoma medicaginis var pinodella (Jones) Boerema = A.pinodella Jones (teleomorph Didymella). This dis ease provides a good ex am ple of the dif fi cul ties in seed in fec tions con trol ling and ep i dem ics of Ascochyta blight causes by patho gens that are highly host-spe cific and are ca pa ble of caus ing se ri ous dam age in a con duc tive envi ron ment. Since the area of pea has sys tem at i cally in creased in some countries of the world, Ascochyta blight of pea has be come one of the most im por tant dis eases in pea pro duc tion. Ex cept of di rect yield losses, Communicated by Ed ward ArseniukPLANT BREEDING AND SEED SCIENCE

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Section: Discussionmentioning

confidence: 99%

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

Furgał-Węgrzycka¹,

Węgrzycki²

2009

Plant Breeding and Seed Science

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“…ITS sequences were clearly heterogenous enough to separate A. pisi from other two species. FarisMokaiesh et al [18] on the basis of PCR-RFLP of IGS whereas Barve et al [7] and Peever et al [36] on the basis of ITS sequences also differentiated A. pisi from A. pinodes and A. pinodella. Moreover, A. pisi showed lower mean/net genetic distance with A. rabiei group than A. pinodes/A.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…This means that ITS region does not separate these two species and there is a need to sequence other regions of their genome. In fact, [7,36] were unable to differentiate these two species on the basis of ITS ribotyping, but could easily differentiate into separate clades on the basis of high mobility group (HMG) domain of MAT gene and glyceraldehyde-3-phosphate dehydrogenase (G3PD) phylogeny. The third species in the Ascochyta blight complex of peas, A. pisi formed a separate clade II which also included As42 (FJ032642), an Ascochyta spp.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…Fatehi et al [20] on basis of mitochondrial genomes alluded them to be evolved from a single, less specific ancestor. Based on HMG phylogeny, Barve et al [7] found that A. pisi was closer to A. rabiei clade than other two species infecting pea. On the basis of G3PD sequencing, Peever et al [36] found A. rabiei and A. pisi to be in the same clade.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…Thus, direct sequence determination provides the most robust data from which phylogenetic reconstruction can proceed with confidence. The most contemporary phylogenetic studies have been based on ITS, mtSSU, mtLSU, b-tubulin, glyceraldehyde-3-phosphate dehydrogenase gene (G3PD), chitin synthase 1 gene (CHS), translation elongation factor 1 alpha gene (EF) and MAT locus sequence analysis, and have supported the organization of Ascochyta species into distinct species groups [7,12,13,20,36].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Genetic Diversity Analysis of Ascochyta Species Associated with Blight Complex of Pea in a Northwestern Hill State of India

2012

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Forty isolates of Ascochyta spp. infecting pea collected from different commercial pea growing areas of Himachal Pradesh were characterized into three species-Ascochyta pinodes, A. pisi and A. pinodella-based on symptoms, morphological and cultural traits. The identity of the isolates was confirmed by studying the rDNA sequence patterns. Based on various cultural and morphological trait combinations, 36 isolates were identified as A. pinodes, one isolate as A. pisi and one isolate as A. pinodella. Whereas two isolates, As3 and As14, could not be placed in any of the species as equal number of characters matched with A. pisi and A. pinodes. However, sequencing of ITS region of these isolates resolved the ambiguity and placed them in A. pinodes. This necessitates ITS sequencing as one of the criteria to establish the taxonomic status of Ascochyta species infecting pea. RAPD, RAMS, and Rep-PCR analysis with 10, 4 and universal ERIC, and BOX-AIR primers categorized different isolates according to their geographical locations. This study established the predominance of A. pinodes in Himachal Pradesh where edible pea is one of the remunerative offseason vegetable crops. This also constitutes first report of genetic variability of Ascochyta species infecting pea in India.

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Mating-Type Structure, Evolution, and Function in Euascomycetes

Debuchy

Turgeon

Growth, Differentiation and Sexuality

158

204

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Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp.

Cited by 105 publications

References 51 publications

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

Identification and Genetic Diversity Analysis of Ascochyta Species Associated with Blight Complex of Pea in a Northwestern Hill State of India

Mating-Type Structure, Evolution, and Function in Euascomycetes

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