Cloning and characterization of Taenia saginata paramyosin cDNA

Abstract

A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.

Molecular Cloning and Characterization of a Paramyosin from Clonorchis sinensis

“…Bacterially expressed rCsPmy was found to bind collagen in a dose-dependent manner. The ability of paramyosin from helminth parasites to bind collagen implies it performs significant, non-muscular functions in host-parasite interactions [11,12,[33][34][35]. This is notable because paramyosin inhibits the classical complement cascade pathway of the host immune system possibly through competitively binding to the collagen-like regions of C1q [16].…”

Neurocysticercosis: relationship between the developmental stage of metacestode present and the titre of specific IgG in the cerebrospinal fluid

“…The immunological studies are usually based on the detection in sera of antibodies toward the causative agent (Taenia solium) or of antigens from the same cestode. The antibody-detection assays used have been based on recombinant proteins or natural antigens from T. solium (Rosas et al, 1986;Tsang et al, 1989;Ito et al, 1998;Ko and Ng, 1998;D'Souza and Hafez, 1999;Ev et al, 1999;Plancarte et al, 1999;Hancock et al, 2003), T. crassiceps (Garcia et al, 1995;Peralta et al, 2002) or T. saginata (Ferrer et al, 2003).…”

In silicocharacterization ofEchinococcus granulosusparamyosin nucleotide sequence for the development of epitope vaccine against cystic echinococcosis

“…Pmy has been demonstrated to be a promising vaccine candidate for control of S. japonicum, S. mansoni (Jiz et al, 2015), Brugia malayi (Nanduri et al, 1989), T. solium (Vazquez et al2001) and T. saginata (Ferrer et al, 2003) infections. These results implied a potential of EgPmy to be an effective vaccine against CE.…”

“…Moreover, Vazquez et al (2001) discovered that the parasite load in mice infected with Taenia solium (T. solium) larvae is reduced by approximately 52 % by immunizing mice with the total length of T. solium recombinant paramyosin. In addition, Ferrer et al (2003) reported that the T. saginata paramyosin was highly recognized by antibodies in 100 % of the sera collected from patients with acute cysticercosis. However, little information is known about their protection effi ciency as vaccines against CE.…”