Cloning and characterization of SREBP-1 and PPAR-α in Japanese seabass Lateolabrax japonicus, and their gene expressions in response to different dietary fatty acid profiles

Dong, Xiaojing; Xu, Houguo; Mai, Kangsen; Xu, Wei; Ai, Qinghui

doi:10.1016/j.cbpb.2014.10.001

Cited by 50 publications

(40 citation statements)

References 60 publications

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“…Two forms of mammalian SREBP-1 have been characterized, SREBP-1a and -1c. However, to date, only a single form of the SREBP-1 gene has been characterized in fish333741, with no exception for the three fishes investigated in the present study. The alignment analysis of the deduced amino acid sequences showed that the SREBP-1 genes from fish were more similar to human SREBP-1a than to human SREBP-1c, indicating that fish SREBP-1 genes likely only possess functions similar to those of human SREBP-1a transcripts3337.…”

Section: Discussionmentioning

confidence: 92%

“…However, to date, only a single form of the SREBP-1 gene has been characterized in fish333741, with no exception for the three fishes investigated in the present study. The alignment analysis of the deduced amino acid sequences showed that the SREBP-1 genes from fish were more similar to human SREBP-1a than to human SREBP-1c, indicating that fish SREBP-1 genes likely only possess functions similar to those of human SREBP-1a transcripts3337. Unlike in mammals, two forms of the PPAR-α gene were characterized in rainbow trout, which was consistent with Japanese seabass, fugu, zebrafish, Japanese medaka, turbot and grass carp334243.…”

Section: Discussionmentioning

confidence: 92%

“…Two transcription factors, SREBP and PPAR, are involved in the regulation of fatty acid biosynthesis in mammals27282930313233, by binding to sterol regulatory elements (SREs)2734 and peroxisome proliferator response elements (PPREs)35, respectively. In fish, previous studies have shown that SREBP-1 is related to fatty acid metabolism3637 and that the gene expression of SREBP-1 and PPAR-α can be regulated by dietary fatty acids33.…”

mentioning

confidence: 99%

“…In fish, previous studies have shown that SREBP-1 is related to fatty acid metabolism3637 and that the gene expression of SREBP-1 and PPAR-α can be regulated by dietary fatty acids33. However, it remains unclear whether these two factors are involved in fatty acid biosynthesis by targeting FADS2.…”

mentioning

confidence: 99%

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Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish

Dong

Tan

Cai

et al. 2017

Sci Rep

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The present study was conducted to explore the mechanisms leading to differences among fishes in the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs). Replacement of fish oil with vegetable oil caused varied degrees of increase in 18-carbon fatty acid content and decrease in n-3 LC-PUFA content in the muscle and liver of rainbow trout (Oncorhynchus mykiss), Japanese seabass (Lateolabrax japonicus) and large yellow croaker (Larimichthys crocea), suggesting that these fishes have differing abilities to biosynthesize LC-PUFAs. Fish oil replacement also led to significantly up-regulated expression of FADS2 and SREBP-1 but different responses of the two PPAR-α homologues in the livers of these three fishes. An in vitro experiment indicated that the basic transcription activity of the FADS2 promoter was significantly higher in rainbow trout than in Japanese seabass or large yellow croaker, which was consistent with their LC-PUFA biosynthetic abilities. In addition, SREBP-1 and PPAR-α up-regulated FADS2 promoter activity. These regulatory effects varied considerably between SREBP-1 and PPAR-α, as well as among the three fishes. Taken together, the differences in regulatory activities of the two transcription factors targeting FADS2 may be responsible for the different LC-PUFA biosynthetic abilities in these three fishes that have adapted to different ambient salinity.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish

Dong

Tan

Cai

et al. 2017

Sci Rep

Self Cite

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“…comunes, se conocen tres isoformas de PPARs, α (alfa), β/δ (beta/delta) y γ (gamma) 32 . PPAR-γ es expresado principalmente en el tejido adiposo y sistema inmune 25 y en músculo e hígado 33 ; participa en la diferenciación de adipocitos y la sensibilidad a la insulina 34 .…”

Section: Enzimas Desaturasasunclassified

Acción de flavonoides sobre la conversión de ácidos grasos poliinsaturados de cadena larga a partir de ácidos grasos esenciales

Valenzuela

Villanueva

2018

Rev. chil. nutr.

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Dietary Polyunsaturated Fatty Acid Supplementations Could Significantly Promote the Δ6 Fatty Acid Desaturase and Fatty Acid Elongase Gene Expression, Long Chain Polyunsaturated Fatty Acids, and Growth of Juvenile Cobia

Zheng

Liu

et al. 2018

Eur. J. Lipid Sci. Technol.

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The experiment investigates the effect of different dietary polyunsaturated fatty acid (PUFA) supplementations on the gene expression of key enzymes for biosynthesizing long chain PUFA (LC‐PUFA), LC‐PUFA formation, and growth performance of juvenile cobia (Rachycentron canadum). It is found that dietary linolenic (LNA) and linoleic acids (LA) supplementations could significantly promote the expression of △6 fatty acid desaturase (FADS2) and fatty acid elongase (ELOVL5) genes in the brain, liver, and muscle of the juveniles; different dietary PUFA supplementations can significantly promote the growth performance and increase the LC‐PUFA in the above organs/tissues of the juveniles; the optimum n‐3/n‐6PUFA ratio was 0.63 in SO + FO diet, in which n‐6PUFAs was 27.4% and n‐3PUFAs 17.4% in the diet, with the supplementation of 3% fish oil and 3% safflower oil. Practical Applications: Cobia is a new species with high econimic value and is developing rapidly. The LC‐PUFA can promote the development, functions of nerves and cell members in fish juveniles, which are an important period of fish. Can juvenile cobia biosynthesize LC‐PUFA from C18:2n‐6 and C18:3n‐3? The results show that dietary LNA and LA supplementations significantly promoted the FADS2 and ELOVL5 gene expression, fatty acids, and growth of cobia juveniles. Dietary LNA and LA supplementations could significantly promote the expression of Δ6 fatty acid desaturase (FADS2) and fatty acid elongase (ELOVL5) genes in the brain, liver, and muscle of juvenile cobia.

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Cloning and characterization of SREBP-1 and PPAR-α in Japanese seabass Lateolabrax japonicus, and their gene expressions in response to different dietary fatty acid profiles

Cited by 50 publications

References 60 publications

Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish

Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish

Acción de flavonoides sobre la conversión de ácidos grasos poliinsaturados de cadena larga a partir de ácidos grasos esenciales

Dietary Polyunsaturated Fatty Acid Supplementations Could Significantly Promote the Δ6 Fatty Acid Desaturase and Fatty Acid Elongase Gene Expression, Long Chain Polyunsaturated Fatty Acids, and Growth of Juvenile Cobia

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