Cloning and Characterization of Resistance Gene Analogues (RGAs) from Piper Nigrum L. cv. Semongok Aman and Piper Colubrinum Link

Tiing, Lau Ee; San, Hwang Siaw; Eng, Lily; Det, Paulus Amin

doi:10.1016/j.apcbee.2012.11.036

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Fresh flowers (1 day after anthesis, DAA) and fruit spikes (14 DAA) (Supplementary material 1) were collected from two biological replicates (two plants) of each pepper variety and snap-frozen in liquid nitrogen before storage at -80°C until use. Total RNA was extracted from 2 g of frozen plant tissue using a modified CTAB method [18]. The RNA was purified and treated with RQ1 RNase-free DNase (Promega, USA) to remove DNA contamination.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrumL.) Varieties

Khew

Harikrishna

Wee

et al. 2020

International Journal of Genomics

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Black pepper (Piper nigrum) is a vital spice crop with uses ranging from culinary to pharmacological applications. However, limited genetic information has constrained the understanding of the molecular regulation of flower and fruit development in black pepper. In this study, a comparison among three different black pepper varieties, Semengok Aman (SA), Kuching (KC), and Semengok 1 (S1), with varying fruit characteristics was used to provide insight on the genetic regulation of flower and fruit development. Next-generation sequencing (NGS) technology was used to determine the flower and fruit transcriptomes by sequencing on an Illumina HiSeq 2500 platform followed by de novo assembly using SOAPdenovo-Trans. The high-quality assembly of 66,906 of unigenes included 64.4% of gene sequences (43,115) with similarity to one or more protein sequences from the GenBank database. Annotation with Blast2Go assigned 37,377 genes to one or more Gene Ontology terms. Of these genes, 5,874 genes were further associated with the biological pathways recorded in the KEGG database. Comparison of flower and fruit transcriptome data from the three different black pepper varieties revealed a large number of DEGs between flower and fruit of the SA variety. Gene Ontology (GO) enrichment analysis further supports functions of DEGs between flower and fruit in the categories of carbohydrate metabolic processes, embryo development, and DNA metabolic processes while the DEGs in fruit relate to biosynthetic process, secondary metabolic process, and catabolic process. The enrichment of DEGs in KEGG pathways was also investigated, and a large number of genes were found to belong to the nucleotide metabolism and carbohydrate metabolism categories. Gene expression profiling of flower formation-related genes reveals that other than regulating the flowering in black pepper, the flowering genes might also be implicated in the fruit development process. Transcriptional analysis of sugar transporter and carbohydrate metabolism genes in different fruit varieties suggested that the carbohydrate metabolism in black pepper fruit is developmentally regulated, and some genes might serve as potential genes for future crop quality improvement. Study on the piperine-related gene expression analysis suggested that lysine-derived products might present in all stages of fruit development, but the transportation was only active at the early stage of fruit development. These results indicate several candidate genes related to the development of flower and fruit in black pepper and provide a resource for future functional analysis and potentially for future crop improvement.

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Section: Methodsmentioning

confidence: 99%

Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrumL.) Varieties

Khew

Harikrishna

Wee

et al. 2020

International Journal of Genomics

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“…As this spice is being widely used in traditional medicine and culinary purposes, P. nigrum L. is the most economically important species among the Piperaceae family. The plants are commercially cultivated in countries such as Malaysia, Brazil, Vietnam, India and Indonesia (Tiing et al, 2012). According to Malaysian Pepper Board (2018), the pepper production of the nation reached 31,073 tonnes with the total export of 11,714 tonnes that worth RM 200.95 million in 2018.…”

Section: Introductionmentioning

confidence: 99%

“…Crop loss due to diseases is one of the primary constraints in increasing the yield of pepper production in Malaysia which results in diminishing of 2% of the pepper cultivation area annually (Krishnamurthy et al, 2010). One of the major diseases is Fusarium wilt, which is also known as slow wilt or yellowing disease (Gordo et al, 2012;Tiing et al, 2012). The disease may cause as high as 32% of annual loss in Indonesia (Mustika, 1990).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak

Tan¹,

Thanh²,

Rafael³

et al. 2021

MJM

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Aims: Piper nigrum L. (black pepper) is an economically important commodity plant in Malaysia, which generated RM 200.95 million from pepper export in the year of 2018. However, the increase in pepper production is restricted by diseases. Fusarium wilt is one of the major diseases of P. nigrum L. The objectives for this study were to isolate Fusarium spp. associated with Fusarium wilt of P. nigrum L. from selected pepper farms in the northwestern region of Sarawak and to characterize the Fusarium spp. isolated morphologically and molecularly. Methodology and results: Fusarium spp. were isolated from diseased root samples. The pathogen was grown on potato dextrose agar (PDA) under dark condition at circa (ca.) 25 °C for morphological characterisation. Molecular characterisation was done by using internal transcribed spacer (ITS). Phylogenetic tree was constructed to study the genetic relationship of the isolates. Fusarium solani, Fusarium oxysporum, Fusarim proliferatum were the three Fusarium species identified. There were variations in morphological characters observed between and among the species, including the colony form, margin, elevation, surface appearance and pigmentation. No distinctive morphological characteristic was specific to a location. In addition, growth rate, macroconidia sporulation rate, and microconidia sporulation rate of the isolates were not correlated. In molecular phylogeny, the three Fusarium species were separated into three distinct clades representing the three identified species. The genetic relatedness between isolates within each species was depicted in the tree. Conclusion, significance and impact of study: Variations were observed among isolates in this study based on morphological and molecular characterization. This study would contribute information on the variations of Fusarium spp. associated with Fusarium wilt of P. nigrum L. from the northwestern region of Sarawak.

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“…Fumigants and nematicides pose a threat to both humans and the ecosystem due to their gradual environmental toxicities (Naz et al, 2020). Efforts to graft P. nigrum shoots onto P. colubrinum rootstocks yielded high initial success but graft incompatibility (Sim and Rosmah, 2011) led to poor plant survival (Lau et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Reducing root galling caused by root knot nematodes in black pepper (<em>Piper nigrum</em> L. cv. ‘Kuching’) through nutrient supplementation

et al. 2022

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Purpose: Root-knot nematodes (RKN) remain one of the leading causes of economic damage in black pepper (Piper nigrum L.) cultivation worldwide. An experiment was conducted to evaluate the response of black pepper plants to RKN when irrigated with three nutrient solutions; Hoagland solution only, Hoagland solution + 0.005 mM potassium silicate solution, and Hoagland solution + 2 mM salicylic acid solution.Research Method: Plants grown in medium-sized polybags were inoculated with RKN and routinely irrigated with the appropriate nutrient solutions. Presence of foliar symptoms of RKN infection on plants, their aerial growth plus root infestation were assessed. Superoxide anion and hydrogen peroxide production from the plant leaves were also detected after inoculation.Findings: Foliar symptoms of RKN infection started manifesting after 81 days from inoculation. Plants without silicate or salicylic acid supplementation displayed the most severe foliar disorder symptoms, highest root galling and the shortest aerial growth. Plants irrigated with potassium silicate had the highest aerial growth and their leaves showed the least oxidative stress when challenged with RKN.Originality/value: From present results, soil amendment with potassium silicate is a promising tool for reducing root galling and promoting aerial growth in RKN-infested black pepper fields.

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Cloning and Characterization of Resistance Gene Analogues (RGAs) from Piper Nigrum L. cv. Semongok Aman and Piper Colubrinum Link

Cited by 6 publications

References 12 publications

Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrumL.) Varieties

Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrumL.) Varieties

Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak

Reducing root galling caused by root knot nematodes in black pepper (<em>Piper nigrum</em> L. cv. ‘Kuching’) through nutrient supplementation

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