Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa

Abstract

A gene encoding phzR was isolated from a phenazine-producing bacterium Pseudomonas aeruginosa 2016NX1. This paper provided the full-length cDNA encoding phzR (GenBank Accession no., MW143078). The cDNA of the phzR contained an open reading frame (ORF) of 714 bp. The potential regulatory elements were predicted in the phzR promoter region. The deduced amino acid sequence of P. aeruginosa phzR showed significant homology to the known phzRs from different organisms. Gene overexpression analysis showed that the phenazine content was improved (44.39%) in comparison to wild-type 2016NX1.

Type Strains of Entomopathogenic Nematode-Symbiotic Bacterium Species, Xenorhabdus szentirmaii (EMC) and X. budapestensis (EMA), Are Exceptional Sources of Non-Ribosomal Templated, Large-Target-Spectral, Thermotolerant-Antimicrobial Peptides (by Both), and Iodinin (by EMC)

“…The Bode Laboratory recently discovered diversity-oriented modifications of the phenazine core through two distinct BGCs in X. szentirmaii. A previously unidentified aldehyde intermediate, which can be modified by multiple enzymatic and non-enzymatic reactions, is a common intermediate bridging the pathways encoded by the respective biosynthetic gene clusters BGCs [ 132 ].…”

“…This is followed by gene U, of unknown function, and opposite transcription, followed by gene V, of unknown function, but similar transcription (orientated as A–F), finally followed by genes G and H, encoding for iodinin biosynthesis, [ 25 ]. and maintaining the same transcription (5′–3′) orientation as A–F [ 132 ]. Although the authors specifically pointed this out, please note that in this pathway, unlike the second, no NRPS-like enzyme-coding gene is represented.…”

“…This indicates a promising reservoir for non-ribosomal synthesized (NRS) peptides with new bioactive effects. Xenorhabdus szentirmaii EMC produces xenofuranones A and B [145][146][147], iodinin [24,86,132], szentiamide [148,149], and phenazines [132]. Xenorhabdus budapestensis NMC-10 produces two novel antimicrobial peptides with antibacterial and actinomycete activities [133].…”