Cloning and characterization of phosphomannose isomerase from sphingomonas chungbukensis DJ77

Abstract

Phosphomannose isomerase (PMI) catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide (EPS) synthesis pathway. The gene encoding PMI in Sphingomonas chungbukensis DJ77 was cloned and expressed in E. coli. The pmi gene is 1,410 nucleotides long and the deduced amino acid sequence shares high homology with other bifunctional proteins that possess both PMI and GDP-mannose pyrophosphorylase (GMP) activities. The sequence analysis of PMI revealed two domains with three conserved motifs: a GMP domain at the N-terminus and a PMI domain at the C-terminus. Enzyme assays using the PMI protein confirmed its bifunctional activity. Both activities required divalent metal ions such as Co

DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response

“…1 ). The conserved VEKP sequence included in the GMP active site is a mannose-1-phosphate binding site of GMP 27 . To examine the relationship between the three DoGMP proteins and other GMP members in plants, a phylogenetic analysis was performed among GMP protein sequences using MEGA 4 28 .…”

Reconstruction and analysis of the genome-scale metabolic model of Lactobacillus casei LC2W

“…Biosynthesis of the characteristic compounds for exopolysaccharide production by L. casei LC2W EPSs, which have been isolated from the skim milk produced by L. casei LC2W, are composed of four monosaccharides, i.e., D-glucose, D-galactose, D-rhamnose, and D-mannose (Ai et al, 2008). These monosaccharides seemed to be released individually by UDP-glucose, UDP-galactose, dTDP-rhamnose, and GDP-mannose (Jensen and Reeves, 2001;Johnson et al, 2005;Tran et al, 2009). The genome annotation indicated that L. casei LC2W could synthesize UDP-glucose, UDP-galactose, and dTDPrhamnose via successive catalytic reactions using the enzymes Pgm, RffH, RfbB, RfbC, RfbD, GtaB, GalE, and GalT (Fig.…”

Molecular Docking Analysis and Biochemical Evaluation of Levansucrase from Sphingobium chungbukense DJ77

“…14,15,34 This bacterium has the potential to produce exopolysaccharides (EPS), which can be used as a gelling agent for various pharmaceutical and industrial processes, because some genes encoding functional enzymes (i.e., phosphomannose isomerase and phosphoglucose isomerase) involved in EPS biosynthesis pathways have been isolated through its genomic analysis. 35,36 EPS levan, which is synthesized by levansucrase, is a fructantype homopolysaccharide with β-(2,6) glycosidic linkages. 37 It is useful in a wide range of applications including food, feed, cosmetics, and medicine due to its uncommon properties, for example, low intrinsic viscosity from other polysaccharides.…”

“…Sphingobium chungbukense DJ77, isolated from contaminated sediment of an industrial complex near Daejon, Republic of Korea, is a Gram-negative bacteria strain. We previously reported that it is useful for the synthesis of sphingolipids and for the degradation of poly- and monocyclic aromatic hydrocarbons such as phenanthrene, anthracene, toluene, m -xylene, phenol, salicylate, benzoate, and p -cresol. ,, This bacterium has the potential to produce exopolysaccharides (EPS), which can be used as a gelling agent for various pharmaceutical and industrial processes, because some genes encoding functional enzymes (i.e., phosphomannose isomerase and phosphoglucose isomerase) involved in EPS biosynthesis pathways have been isolated through its genomic analysis. , …”